EndoBind detects endogenous protein-protein interactions in real time

Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.

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Détails bibliographiques
Auteurs principaux:	Anke Bill, Sheryll Espinola, Daniel Guthy, Jacob R. Haling, Mylene Lanter, Min Lu, Anthony Marelli, Angelica Mendiola, Loren Miraglia, Brandon L. Taylor, Leonardo Vargas, Anthony P. Orth, Frederick J. King
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a5
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spelling	oai:doaj.org-article:e6bdb53a936c41a9bbda86784fa7f7a52021-12-02T18:34:00ZEndoBind detects endogenous protein-protein interactions in real time10.1038/s42003-021-02600-52399-3642https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a52021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02600-5https://doaj.org/toc/2399-3642Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.Anke BillSheryll EspinolaDaniel GuthyJacob R. HalingMylene LanterMin LuAnthony MarelliAngelica MendiolaLoren MiragliaBrandon L. TaylorLeonardo VargasAnthony P. OrthFrederick J. KingNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King EndoBind detects endogenous protein-protein interactions in real time
description	Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.
format	article
author	Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King
author_facet	Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King
author_sort	Anke Bill
title	EndoBind detects endogenous protein-protein interactions in real time
title_short	EndoBind detects endogenous protein-protein interactions in real time
title_full	EndoBind detects endogenous protein-protein interactions in real time
title_fullStr	EndoBind detects endogenous protein-protein interactions in real time
title_full_unstemmed	EndoBind detects endogenous protein-protein interactions in real time
title_sort	endobind detects endogenous protein-protein interactions in real time
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a5
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EndoBind detects endogenous protein-protein interactions in real time

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