EndoBind detects endogenous protein-protein interactions in real time
Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.
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Nature Portfolio
2021
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oai:doaj.org-article:e6bdb53a936c41a9bbda86784fa7f7a52021-12-02T18:34:00ZEndoBind detects endogenous protein-protein interactions in real time10.1038/s42003-021-02600-52399-3642https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a52021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02600-5https://doaj.org/toc/2399-3642Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.Anke BillSheryll EspinolaDaniel GuthyJacob R. HalingMylene LanterMin LuAnthony MarelliAngelica MendiolaLoren MiragliaBrandon L. TaylorLeonardo VargasAnthony P. OrthFrederick J. KingNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-6 (2021) |
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DOAJ |
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DOAJ |
language |
EN |
topic |
Biology (General) QH301-705.5 |
spellingShingle |
Biology (General) QH301-705.5 Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King EndoBind detects endogenous protein-protein interactions in real time |
description |
Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively. |
format |
article |
author |
Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King |
author_facet |
Anke Bill Sheryll Espinola Daniel Guthy Jacob R. Haling Mylene Lanter Min Lu Anthony Marelli Angelica Mendiola Loren Miraglia Brandon L. Taylor Leonardo Vargas Anthony P. Orth Frederick J. King |
author_sort |
Anke Bill |
title |
EndoBind detects endogenous protein-protein interactions in real time |
title_short |
EndoBind detects endogenous protein-protein interactions in real time |
title_full |
EndoBind detects endogenous protein-protein interactions in real time |
title_fullStr |
EndoBind detects endogenous protein-protein interactions in real time |
title_full_unstemmed |
EndoBind detects endogenous protein-protein interactions in real time |
title_sort |
endobind detects endogenous protein-protein interactions in real time |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a5 |
work_keys_str_mv |
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1718377952980238336 |