EndoBind detects endogenous protein-protein interactions in real time

Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.

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Autores principales: Anke Bill, Sheryll Espinola, Daniel Guthy, Jacob R. Haling, Mylene Lanter, Min Lu, Anthony Marelli, Angelica Mendiola, Loren Miraglia, Brandon L. Taylor, Leonardo Vargas, Anthony P. Orth, Frederick J. King
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a5
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spelling oai:doaj.org-article:e6bdb53a936c41a9bbda86784fa7f7a52021-12-02T18:34:00ZEndoBind detects endogenous protein-protein interactions in real time10.1038/s42003-021-02600-52399-3642https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a52021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02600-5https://doaj.org/toc/2399-3642Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.Anke BillSheryll EspinolaDaniel GuthyJacob R. HalingMylene LanterMin LuAnthony MarelliAngelica MendiolaLoren MiragliaBrandon L. TaylorLeonardo VargasAnthony P. OrthFrederick J. KingNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-6 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Anke Bill
Sheryll Espinola
Daniel Guthy
Jacob R. Haling
Mylene Lanter
Min Lu
Anthony Marelli
Angelica Mendiola
Loren Miraglia
Brandon L. Taylor
Leonardo Vargas
Anthony P. Orth
Frederick J. King
EndoBind detects endogenous protein-protein interactions in real time
description Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.
format article
author Anke Bill
Sheryll Espinola
Daniel Guthy
Jacob R. Haling
Mylene Lanter
Min Lu
Anthony Marelli
Angelica Mendiola
Loren Miraglia
Brandon L. Taylor
Leonardo Vargas
Anthony P. Orth
Frederick J. King
author_facet Anke Bill
Sheryll Espinola
Daniel Guthy
Jacob R. Haling
Mylene Lanter
Min Lu
Anthony Marelli
Angelica Mendiola
Loren Miraglia
Brandon L. Taylor
Leonardo Vargas
Anthony P. Orth
Frederick J. King
author_sort Anke Bill
title EndoBind detects endogenous protein-protein interactions in real time
title_short EndoBind detects endogenous protein-protein interactions in real time
title_full EndoBind detects endogenous protein-protein interactions in real time
title_fullStr EndoBind detects endogenous protein-protein interactions in real time
title_full_unstemmed EndoBind detects endogenous protein-protein interactions in real time
title_sort endobind detects endogenous protein-protein interactions in real time
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/e6bdb53a936c41a9bbda86784fa7f7a5
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