Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.

If insufficiently treated, Lyme borreliosis can evolve into an inflammatory disorder affecting skin, joints, and the CNS. Early innate immunity may determine host responses targeting infection. Thus, we sought to characterize the immediate cytokine storm associated with exposure of PBMC to moderate...

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Autores principales:	Malte Bachmann, Katharina Horn, Ina Rudloff, Itamar Goren, Martin Holdener, Urs Christen, Nicole Darsow, Klaus-Peter Hunfeld, Ulrike Koehl, Peter Kind, Josef Pfeilschifter, Peter Kraiczy, Heiko Mühl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e6c088080be7423fa78c20ce81794829
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spelling	oai:doaj.org-article:e6c088080be7423fa78c20ce817948292021-11-18T06:03:50ZEarly production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.1553-73661553-737410.1371/journal.ppat.1001144https://doaj.org/article/e6c088080be7423fa78c20ce817948292010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976193/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374If insufficiently treated, Lyme borreliosis can evolve into an inflammatory disorder affecting skin, joints, and the CNS. Early innate immunity may determine host responses targeting infection. Thus, we sought to characterize the immediate cytokine storm associated with exposure of PBMC to moderate levels of live Borrelia burgdorferi. Since Th17 cytokines are connected to host defense against extracellular bacteria, we focused on interleukin (IL)-17 and IL-22. Here, we report that, despite induction of inflammatory cytokines including IL-23, IL-17 remained barely detectable in response to B. burgdorferi. In contrast, T cell-dependent expression of IL-22 became evident within 10 h of exposure to the spirochetes. This dichotomy was unrelated to interferon-γ but to a large part dependent on caspase-1 and IL-1 bioactivity derived from monocytes. In fact, IL-1β as a single stimulus induced IL-22 but not IL-17. Neutrophils display antibacterial activity against B. burgdorferi, particularly when opsonized by antibodies. Since neutrophilic inflammation, indicative of IL-17 bioactivity, is scarcely observed in Erythema migrans, a manifestation of skin inflammation after infection, protective and antibacterial properties of IL-22 may close this gap and serve essential functions in the initial phase of spirochete infection.Malte BachmannKatharina HornIna RudloffItamar GorenMartin HoldenerUrs ChristenNicole DarsowKlaus-Peter HunfeldUlrike KoehlPeter KindJosef PfeilschifterPeter KraiczyHeiko MühlPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 10, p e1001144 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Malte Bachmann Katharina Horn Ina Rudloff Itamar Goren Martin Holdener Urs Christen Nicole Darsow Klaus-Peter Hunfeld Ulrike Koehl Peter Kind Josef Pfeilschifter Peter Kraiczy Heiko Mühl Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
description	If insufficiently treated, Lyme borreliosis can evolve into an inflammatory disorder affecting skin, joints, and the CNS. Early innate immunity may determine host responses targeting infection. Thus, we sought to characterize the immediate cytokine storm associated with exposure of PBMC to moderate levels of live Borrelia burgdorferi. Since Th17 cytokines are connected to host defense against extracellular bacteria, we focused on interleukin (IL)-17 and IL-22. Here, we report that, despite induction of inflammatory cytokines including IL-23, IL-17 remained barely detectable in response to B. burgdorferi. In contrast, T cell-dependent expression of IL-22 became evident within 10 h of exposure to the spirochetes. This dichotomy was unrelated to interferon-γ but to a large part dependent on caspase-1 and IL-1 bioactivity derived from monocytes. In fact, IL-1β as a single stimulus induced IL-22 but not IL-17. Neutrophils display antibacterial activity against B. burgdorferi, particularly when opsonized by antibodies. Since neutrophilic inflammation, indicative of IL-17 bioactivity, is scarcely observed in Erythema migrans, a manifestation of skin inflammation after infection, protective and antibacterial properties of IL-22 may close this gap and serve essential functions in the initial phase of spirochete infection.
format	article
author	Malte Bachmann Katharina Horn Ina Rudloff Itamar Goren Martin Holdener Urs Christen Nicole Darsow Klaus-Peter Hunfeld Ulrike Koehl Peter Kind Josef Pfeilschifter Peter Kraiczy Heiko Mühl
author_facet	Malte Bachmann Katharina Horn Ina Rudloff Itamar Goren Martin Holdener Urs Christen Nicole Darsow Klaus-Peter Hunfeld Ulrike Koehl Peter Kind Josef Pfeilschifter Peter Kraiczy Heiko Mühl
author_sort	Malte Bachmann
title	Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
title_short	Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
title_full	Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
title_fullStr	Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
title_full_unstemmed	Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.
title_sort	early production of il-22 but not il-17 by peripheral blood mononuclear cells exposed to live borrelia burgdorferi: the role of monocytes and interleukin-1.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e6c088080be7423fa78c20ce81794829
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Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1.

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