CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

During angiogenesis, cell adhesion molecules expressed on the endothelial cell surface promote the growth and survival of newly forming vessels. Hence, elucidation of the signaling pathways activated by cell-to-matrix adhesion may assist in the discovery of new targets to be used in antiangiogenic t...

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Autores principales:	Stefano Barbera, Luisa Raucci, Roberta Lugano, Gian Marco Tosi, Anna Dimberg, Annalisa Santucci, Federico Galvagni, Maurizio Orlandini
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Src Cbl Crk Rac1 Cdc42 RhoA Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e6c7b607c7ed4b4281652e459054c523
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id	oai:doaj.org-article:e6c7b607c7ed4b4281652e459054c523
record_format	dspace
spelling	oai:doaj.org-article:e6c7b607c7ed4b4281652e459054c5232021-11-25T17:56:27ZCD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells10.3390/ijms2222124171422-00671661-6596https://doaj.org/article/e6c7b607c7ed4b4281652e459054c5232021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12417https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067During angiogenesis, cell adhesion molecules expressed on the endothelial cell surface promote the growth and survival of newly forming vessels. Hence, elucidation of the signaling pathways activated by cell-to-matrix adhesion may assist in the discovery of new targets to be used in antiangiogenic therapy. In proliferating endothelial cells, the single-pass transmembrane glycoprotein CD93 has recently emerged as an important endothelial cell adhesion molecule regulating vascular maturation. In this study, we unveil a signaling pathway triggered by CD93 that regulates actin cytoskeletal dynamics responsible of endothelial cell adhesion. We show that the Src-dependent phosphorylation of CD93 and the adaptor protein Cbl leads to the recruitment of Crk, which works as a downstream integrator in the CD93-mediated signaling. Moreover, confocal microscopy analysis of FRET-based biosensors shows that CD93 drives the coordinated activation of Rac1 and RhoA at the cell edge of spreading cells, thus promoting the establishment of cell polarity and adhesion required for cell motility.Stefano BarberaLuisa RaucciRoberta LuganoGian Marco TosiAnna DimbergAnnalisa SantucciFederico GalvagniMaurizio OrlandiniMDPI AGarticleSrcCblCrkRac1Cdc42RhoABiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12417, p 12417 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Src Cbl Crk Rac1 Cdc42 RhoA Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Src Cbl Crk Rac1 Cdc42 RhoA Biology (General) QH301-705.5 Chemistry QD1-999 Stefano Barbera Luisa Raucci Roberta Lugano Gian Marco Tosi Anna Dimberg Annalisa Santucci Federico Galvagni Maurizio Orlandini CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
description	During angiogenesis, cell adhesion molecules expressed on the endothelial cell surface promote the growth and survival of newly forming vessels. Hence, elucidation of the signaling pathways activated by cell-to-matrix adhesion may assist in the discovery of new targets to be used in antiangiogenic therapy. In proliferating endothelial cells, the single-pass transmembrane glycoprotein CD93 has recently emerged as an important endothelial cell adhesion molecule regulating vascular maturation. In this study, we unveil a signaling pathway triggered by CD93 that regulates actin cytoskeletal dynamics responsible of endothelial cell adhesion. We show that the Src-dependent phosphorylation of CD93 and the adaptor protein Cbl leads to the recruitment of Crk, which works as a downstream integrator in the CD93-mediated signaling. Moreover, confocal microscopy analysis of FRET-based biosensors shows that CD93 drives the coordinated activation of Rac1 and RhoA at the cell edge of spreading cells, thus promoting the establishment of cell polarity and adhesion required for cell motility.
format	article
author	Stefano Barbera Luisa Raucci Roberta Lugano Gian Marco Tosi Anna Dimberg Annalisa Santucci Federico Galvagni Maurizio Orlandini
author_facet	Stefano Barbera Luisa Raucci Roberta Lugano Gian Marco Tosi Anna Dimberg Annalisa Santucci Federico Galvagni Maurizio Orlandini
author_sort	Stefano Barbera
title	CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
title_short	CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
title_full	CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
title_fullStr	CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
title_full_unstemmed	CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
title_sort	cd93 signaling via rho proteins drives cytoskeletal remodeling in spreading endothelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e6c7b607c7ed4b4281652e459054c523
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CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

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