A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond

As key effector cells of the innate immune response, neutrophils are rapidly deployed to sites of inflammation where they deliver a payload of potent effector mechanisms that are essential for host defense against pathogens as well as tissue homeostasis. In addition, neutrophils are central contribu...

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Autores principales:	Diana Changirwa, Jared Schlechte, Braedon McDonald
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	neutrophils intravital microscopy organ-on-a-chip single cell transcriptomics proteomics mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e6ca530c24aa4d2c9ca23166e6365a2c
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spelling	oai:doaj.org-article:e6ca530c24aa4d2c9ca23166e6365a2c2021-11-11T15:28:29ZA Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond10.3390/cancers132153312072-6694https://doaj.org/article/e6ca530c24aa4d2c9ca23166e6365a2c2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5331https://doaj.org/toc/2072-6694As key effector cells of the innate immune response, neutrophils are rapidly deployed to sites of inflammation where they deliver a payload of potent effector mechanisms that are essential for host defense against pathogens as well as tissue homeostasis. In addition, neutrophils are central contributors to the pathogenesis of a vast spectrum of inflammatory, degenerative, and neoplastic diseases. As our understanding of neutrophils in health and disease continually expands, so too does our appreciation of their complex and dynamic nature in vivo; from development, maturation, and trafficking to cellular heterogeneity and functional plasticity. Therefore, contemporary neutrophil research relies on multiple complementary methodologies to perform integrated analysis of neutrophil phenotypic heterogeneity, organ- and stimulus-specific trafficking mechanisms, as well as tailored effector functions in vivo. This review discusses established and emerging technologies used to study neutrophils, with a focus on in vivo imaging in animal models, as well as next-generation ex vivo model systems to study mechanisms of neutrophil function. Furthermore, we discuss how high-dimensional single-cell analysis technologies are driving a renaissance in neutrophil biology by redefining our understanding of neutrophil development, heterogeneity, and functional plasticity. Finally, we discuss innovative applications and emerging opportunities to integrate these high-dimensional, multi-modal techniques to deepen our understanding of neutrophils in cancer research and beyond.Diana ChangirwaJared SchlechteBraedon McDonaldMDPI AGarticleneutrophilsintravital microscopyorgan-on-a-chipsingle cell transcriptomicsproteomicsmass cytometryNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5331, p 5331 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neutrophils intravital microscopy organ-on-a-chip single cell transcriptomics proteomics mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	neutrophils intravital microscopy organ-on-a-chip single cell transcriptomics proteomics mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Diana Changirwa Jared Schlechte Braedon McDonald A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
description	As key effector cells of the innate immune response, neutrophils are rapidly deployed to sites of inflammation where they deliver a payload of potent effector mechanisms that are essential for host defense against pathogens as well as tissue homeostasis. In addition, neutrophils are central contributors to the pathogenesis of a vast spectrum of inflammatory, degenerative, and neoplastic diseases. As our understanding of neutrophils in health and disease continually expands, so too does our appreciation of their complex and dynamic nature in vivo; from development, maturation, and trafficking to cellular heterogeneity and functional plasticity. Therefore, contemporary neutrophil research relies on multiple complementary methodologies to perform integrated analysis of neutrophil phenotypic heterogeneity, organ- and stimulus-specific trafficking mechanisms, as well as tailored effector functions in vivo. This review discusses established and emerging technologies used to study neutrophils, with a focus on in vivo imaging in animal models, as well as next-generation ex vivo model systems to study mechanisms of neutrophil function. Furthermore, we discuss how high-dimensional single-cell analysis technologies are driving a renaissance in neutrophil biology by redefining our understanding of neutrophil development, heterogeneity, and functional plasticity. Finally, we discuss innovative applications and emerging opportunities to integrate these high-dimensional, multi-modal techniques to deepen our understanding of neutrophils in cancer research and beyond.
format	article
author	Diana Changirwa Jared Schlechte Braedon McDonald
author_facet	Diana Changirwa Jared Schlechte Braedon McDonald
author_sort	Diana Changirwa
title	A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
title_short	A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
title_full	A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
title_fullStr	A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
title_full_unstemmed	A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond
title_sort	multi-modal toolkit for studying neutrophils in cancer and beyond
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e6ca530c24aa4d2c9ca23166e6365a2c
work_keys_str_mv	AT dianachangirwa amultimodaltoolkitforstudyingneutrophilsincancerandbeyond AT jaredschlechte amultimodaltoolkitforstudyingneutrophilsincancerandbeyond AT braedonmcdonald amultimodaltoolkitforstudyingneutrophilsincancerandbeyond AT dianachangirwa multimodaltoolkitforstudyingneutrophilsincancerandbeyond AT jaredschlechte multimodaltoolkitforstudyingneutrophilsincancerandbeyond AT braedonmcdonald multimodaltoolkitforstudyingneutrophilsincancerandbeyond
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A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond

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