Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

Photodynamic therapy (PDT) mediated by Fotoenticine<sup>®</sup> (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium <i>Streptococcus mutans</i>. However, its antimicrobial effects must be investigated...

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Autores principales:	Maíra Terra Garcia, Rafael Araújo da Costa Ward, Nathália Maria Ferreira Gonçalves, Lara Luise Castro Pedroso, José Vieira da Silva Neto, Juliana Ferreira Strixino, Juliana Campos Junqueira
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	photodynamic therapy dental caries oral biofilms Fotoenticine chlorine methylene blue Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/e6cb3e5bbc924a4681a7280728b725d2
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spelling	oai:doaj.org-article:e6cb3e5bbc924a4681a7280728b725d22021-11-25T18:41:41ZSusceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine10.3390/pharmaceutics131119071999-4923https://doaj.org/article/e6cb3e5bbc924a4681a7280728b725d22021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1907https://doaj.org/toc/1999-4923Photodynamic therapy (PDT) mediated by Fotoenticine<sup>®</sup> (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium <i>Streptococcus mutans</i>. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of <i>mutans</i> streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.Maíra Terra GarciaRafael Araújo da Costa WardNathália Maria Ferreira GonçalvesLara Luise Castro PedrosoJosé Vieira da Silva NetoJuliana Ferreira StrixinoJuliana Campos JunqueiraMDPI AGarticlephotodynamic therapydental cariesoral biofilmsFotoenticinechlorinemethylene bluePharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1907, p 1907 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photodynamic therapy dental caries oral biofilms Fotoenticine chlorine methylene blue Pharmacy and materia medica RS1-441
spellingShingle	photodynamic therapy dental caries oral biofilms Fotoenticine chlorine methylene blue Pharmacy and materia medica RS1-441 Maíra Terra Garcia Rafael Araújo da Costa Ward Nathália Maria Ferreira Gonçalves Lara Luise Castro Pedroso José Vieira da Silva Neto Juliana Ferreira Strixino Juliana Campos Junqueira Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
description	Photodynamic therapy (PDT) mediated by Fotoenticine<sup>®</sup> (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium <i>Streptococcus mutans</i>. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of <i>mutans</i> streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.
format	article
author	Maíra Terra Garcia Rafael Araújo da Costa Ward Nathália Maria Ferreira Gonçalves Lara Luise Castro Pedroso José Vieira da Silva Neto Juliana Ferreira Strixino Juliana Campos Junqueira
author_facet	Maíra Terra Garcia Rafael Araújo da Costa Ward Nathália Maria Ferreira Gonçalves Lara Luise Castro Pedroso José Vieira da Silva Neto Juliana Ferreira Strixino Juliana Campos Junqueira
author_sort	Maíra Terra Garcia
title	Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
title_short	Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
title_full	Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
title_fullStr	Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
title_full_unstemmed	Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
title_sort	susceptibility of dental caries microcosm biofilms to photodynamic therapy mediated by fotoenticine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e6cb3e5bbc924a4681a7280728b725d2
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Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

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