A non-invasive method to generate induced pluripotent stem cells from primate urine

Abstract Comparing the molecular and cellular properties among primates is crucial to better understand human evolution and biology. However, it is difficult or ethically impossible to collect matched tissues from many primates, especially during development. An alternative is to model different cel...

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Autores principales:	Johanna Geuder, Lucas E. Wange, Aleksandar Janjic, Jessica Radmer, Philipp Janssen, Johannes W. Bagnoli, Stefan Müller, Artur Kaul, Mari Ohnuki, Wolfgang Enard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6cd43e76e3d46b8af6aa9df436d6740
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spelling	oai:doaj.org-article:e6cd43e76e3d46b8af6aa9df436d67402021-12-02T14:27:02ZA non-invasive method to generate induced pluripotent stem cells from primate urine10.1038/s41598-021-82883-02045-2322https://doaj.org/article/e6cd43e76e3d46b8af6aa9df436d67402021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82883-0https://doaj.org/toc/2045-2322Abstract Comparing the molecular and cellular properties among primates is crucial to better understand human evolution and biology. However, it is difficult or ethically impossible to collect matched tissues from many primates, especially during development. An alternative is to model different cell types and their development using induced pluripotent stem cells (iPSCs). These can be generated from many tissue sources, but non-invasive sampling would decisively broaden the spectrum of non-human primates that can be investigated. Here, we report the generation of primate iPSCs from urine samples. We first validate and optimize the procedure using human urine samples and show that suspension- Sendai Virus transduction of reprogramming factors into urinary cells efficiently generates integration-free iPSCs, which maintain their pluripotency under feeder-free culture conditions. We demonstrate that this method is also applicable to gorilla and orangutan urinary cells isolated from a non-sterile zoo floor. We characterize the urinary cells, iPSCs and derived neural progenitor cells using karyotyping, immunohistochemistry, differentiation assays and RNA-sequencing. We show that the urine-derived human iPSCs are indistinguishable from well characterized PBMC-derived human iPSCs and that the gorilla and orangutan iPSCs are well comparable to the human iPSCs. In summary, this study introduces a novel and efficient approach to non-invasively generate iPSCs from primate urine. This will extend the zoo of species available for a comparative approach to molecular and cellular phenotypes.Johanna GeuderLucas E. WangeAleksandar JanjicJessica RadmerPhilipp JanssenJohannes W. BagnoliStefan MüllerArtur KaulMari OhnukiWolfgang EnardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johanna Geuder Lucas E. Wange Aleksandar Janjic Jessica Radmer Philipp Janssen Johannes W. Bagnoli Stefan Müller Artur Kaul Mari Ohnuki Wolfgang Enard A non-invasive method to generate induced pluripotent stem cells from primate urine
description	Abstract Comparing the molecular and cellular properties among primates is crucial to better understand human evolution and biology. However, it is difficult or ethically impossible to collect matched tissues from many primates, especially during development. An alternative is to model different cell types and their development using induced pluripotent stem cells (iPSCs). These can be generated from many tissue sources, but non-invasive sampling would decisively broaden the spectrum of non-human primates that can be investigated. Here, we report the generation of primate iPSCs from urine samples. We first validate and optimize the procedure using human urine samples and show that suspension- Sendai Virus transduction of reprogramming factors into urinary cells efficiently generates integration-free iPSCs, which maintain their pluripotency under feeder-free culture conditions. We demonstrate that this method is also applicable to gorilla and orangutan urinary cells isolated from a non-sterile zoo floor. We characterize the urinary cells, iPSCs and derived neural progenitor cells using karyotyping, immunohistochemistry, differentiation assays and RNA-sequencing. We show that the urine-derived human iPSCs are indistinguishable from well characterized PBMC-derived human iPSCs and that the gorilla and orangutan iPSCs are well comparable to the human iPSCs. In summary, this study introduces a novel and efficient approach to non-invasively generate iPSCs from primate urine. This will extend the zoo of species available for a comparative approach to molecular and cellular phenotypes.
format	article
author	Johanna Geuder Lucas E. Wange Aleksandar Janjic Jessica Radmer Philipp Janssen Johannes W. Bagnoli Stefan Müller Artur Kaul Mari Ohnuki Wolfgang Enard
author_facet	Johanna Geuder Lucas E. Wange Aleksandar Janjic Jessica Radmer Philipp Janssen Johannes W. Bagnoli Stefan Müller Artur Kaul Mari Ohnuki Wolfgang Enard
author_sort	Johanna Geuder
title	A non-invasive method to generate induced pluripotent stem cells from primate urine
title_short	A non-invasive method to generate induced pluripotent stem cells from primate urine
title_full	A non-invasive method to generate induced pluripotent stem cells from primate urine
title_fullStr	A non-invasive method to generate induced pluripotent stem cells from primate urine
title_full_unstemmed	A non-invasive method to generate induced pluripotent stem cells from primate urine
title_sort	non-invasive method to generate induced pluripotent stem cells from primate urine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e6cd43e76e3d46b8af6aa9df436d6740
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A non-invasive method to generate induced pluripotent stem cells from primate urine

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