Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon

Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. Howe...

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Autores principales:	Jordi Mayneris-Perxachs, José Maria Moreno-Navarrete, Marta Ballanti, Giovanni Monteleone, Omero Alessandro Paoluzi, Geltrude Mingrone, Philippe Lefebvre, Bart Staels, Massimo Federici, Josep Puig, Josep Garre, Rafael Ramos, José-Manuel Fernández-Real
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Lipidomics Metabolomics Omega-3 fatty acids SARS-CoV-2 Viral receptors Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e6d9828765e5476d93963cd04887d8c5
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spelling	oai:doaj.org-article:e6d9828765e5476d93963cd04887d8c52021-11-20T05:05:24ZLipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon2001-037010.1016/j.csbj.2021.11.007https://doaj.org/article/e6d9828765e5476d93963cd04887d8c52021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004724https://doaj.org/toc/2001-0370Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. However, emerging research has identified novel mediators such as DPP9, TYK2, and CCR2, all playing a critical role in inflammation. We evaluated the expression of SARS-CoV-2 receptors in peripheral leukocytes (n = 469), jejunum (n = 30), and colon (n = 37) of three independent cohorts by real-time PCR, RNA-sequencing, and microarray transcriptomics. We also performed HPCL-MS/MS lipidomics and metabolomics analyses to identify signatures linked to SARS-CoV-2 receptors. We found markedly higher peripheral leukocytes ACE2 expression levels in women compared to men, whereas the intestinal expression of TMPRSS2 was positively associated with BMI. Consistent lipidomics signatures associated with the expression of these mediators were found in both tissues and peripheral leukocytes involving n-3 long-chain PUFAs and arachidonic acid-derived eicosanoids, which play a key role in the regulation of inflammation and may interfere with viral entry and replication. Medium- and long-chain hydroxy acids, which have shown to interfere in viral replication, were also liked to SARS-CoV2 receptors. Gonadal steroids were also associated with the expression of some of these receptors, even after controlling for sex. The expression of SARS-CoV2 receptors was associated with several metabolic and nutritional traits in different cell types. This information may be useful in the design of potential therapies targeted at coronavirus entry.Jordi Mayneris-PerxachsJosé Maria Moreno-NavarreteMarta BallantiGiovanni MonteleoneOmero Alessandro PaoluziGeltrude MingronePhilippe LefebvreBart StaelsMassimo FedericiJosep PuigJosep GarreRafael RamosJosé-Manuel Fernández-RealElsevierarticleLipidomicsMetabolomicsOmega-3 fatty acidsSARS-CoV-2Viral receptorsBiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 6080-6089 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lipidomics Metabolomics Omega-3 fatty acids SARS-CoV-2 Viral receptors Biotechnology TP248.13-248.65
spellingShingle	Lipidomics Metabolomics Omega-3 fatty acids SARS-CoV-2 Viral receptors Biotechnology TP248.13-248.65 Jordi Mayneris-Perxachs José Maria Moreno-Navarrete Marta Ballanti Giovanni Monteleone Omero Alessandro Paoluzi Geltrude Mingrone Philippe Lefebvre Bart Staels Massimo Federici Josep Puig Josep Garre Rafael Ramos José-Manuel Fernández-Real Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
description	Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. However, emerging research has identified novel mediators such as DPP9, TYK2, and CCR2, all playing a critical role in inflammation. We evaluated the expression of SARS-CoV-2 receptors in peripheral leukocytes (n = 469), jejunum (n = 30), and colon (n = 37) of three independent cohorts by real-time PCR, RNA-sequencing, and microarray transcriptomics. We also performed HPCL-MS/MS lipidomics and metabolomics analyses to identify signatures linked to SARS-CoV-2 receptors. We found markedly higher peripheral leukocytes ACE2 expression levels in women compared to men, whereas the intestinal expression of TMPRSS2 was positively associated with BMI. Consistent lipidomics signatures associated with the expression of these mediators were found in both tissues and peripheral leukocytes involving n-3 long-chain PUFAs and arachidonic acid-derived eicosanoids, which play a key role in the regulation of inflammation and may interfere with viral entry and replication. Medium- and long-chain hydroxy acids, which have shown to interfere in viral replication, were also liked to SARS-CoV2 receptors. Gonadal steroids were also associated with the expression of some of these receptors, even after controlling for sex. The expression of SARS-CoV2 receptors was associated with several metabolic and nutritional traits in different cell types. This information may be useful in the design of potential therapies targeted at coronavirus entry.
format	article
author	Jordi Mayneris-Perxachs José Maria Moreno-Navarrete Marta Ballanti Giovanni Monteleone Omero Alessandro Paoluzi Geltrude Mingrone Philippe Lefebvre Bart Staels Massimo Federici Josep Puig Josep Garre Rafael Ramos José-Manuel Fernández-Real
author_facet	Jordi Mayneris-Perxachs José Maria Moreno-Navarrete Marta Ballanti Giovanni Monteleone Omero Alessandro Paoluzi Geltrude Mingrone Philippe Lefebvre Bart Staels Massimo Federici Josep Puig Josep Garre Rafael Ramos José-Manuel Fernández-Real
author_sort	Jordi Mayneris-Perxachs
title	Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
title_short	Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
title_full	Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
title_fullStr	Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
title_full_unstemmed	Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon
title_sort	lipidomics and metabolomics signatures of sars-cov-2 mediators/receptors in peripheral leukocytes, jejunum and colon
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e6d9828765e5476d93963cd04887d8c5
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Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon

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