Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

Abstract The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In...

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Autores principales:	Wenbo Jiang, Julius Wong, Hyon-Xhi Tan, Hannah G. Kelly, Paul G. Whitney, Ian Barr, Daniel S. Layton, Stephen J. Kent, Adam K. Wheatley, Jennifer A. Juno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6d9e1d491174cc6959c3ae7052b2216
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spelling	oai:doaj.org-article:e6d9e1d491174cc6959c3ae7052b22162021-12-02T13:50:50ZScreening and development of monoclonal antibodies for identification of ferret T follicular helper cells10.1038/s41598-021-81389-z2045-2322https://doaj.org/article/e6d9e1d491174cc6959c3ae7052b22162021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81389-zhttps://doaj.org/toc/2045-2322Abstract The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.Wenbo JiangJulius WongHyon-Xhi TanHannah G. KellyPaul G. WhitneyIan BarrDaniel S. LaytonStephen J. KentAdam K. WheatleyJennifer A. JunoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenbo Jiang Julius Wong Hyon-Xhi Tan Hannah G. Kelly Paul G. Whitney Ian Barr Daniel S. Layton Stephen J. Kent Adam K. Wheatley Jennifer A. Juno Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
description	Abstract The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.
format	article
author	Wenbo Jiang Julius Wong Hyon-Xhi Tan Hannah G. Kelly Paul G. Whitney Ian Barr Daniel S. Layton Stephen J. Kent Adam K. Wheatley Jennifer A. Juno
author_facet	Wenbo Jiang Julius Wong Hyon-Xhi Tan Hannah G. Kelly Paul G. Whitney Ian Barr Daniel S. Layton Stephen J. Kent Adam K. Wheatley Jennifer A. Juno
author_sort	Wenbo Jiang
title	Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
title_short	Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
title_full	Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
title_fullStr	Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
title_full_unstemmed	Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells
title_sort	screening and development of monoclonal antibodies for identification of ferret t follicular helper cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e6d9e1d491174cc6959c3ae7052b2216
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Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

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