Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice

Hsin-Ying Wu,1,* Kun-Ju Lin,2,* Ping-Yen Wang,1 Chi-Wen Lin,3 Hong-Wei Yang,3 Chen-Chi M Ma,3 Yu-Jen Lu,4 Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; 2Animal Molecular Imaging Center and Depart...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wu HY, Lin KJ, Wang PY, Lin CW, Yang HW, Ma CC, Lu YJ, Jan TR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e6db9041fcef45cc9d0a9541bf00b56d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e6db9041fcef45cc9d0a9541bf00b56d
record_format	dspace
spelling	oai:doaj.org-article:e6db9041fcef45cc9d0a9541bf00b56d2021-12-02T08:07:46ZPolyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice1178-2013https://doaj.org/article/e6db9041fcef45cc9d0a9541bf00b56d2014-09-01T00:00:00Zhttp://www.dovepress.com/polyethylene-glycol-coated-graphene-oxide-attenuates-antigen-specific--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Hsin-Ying Wu,1,* Kun-Ju Lin,2,* Ping-Yen Wang,1 Chi-Wen Lin,3 Hong-Wei Yang,3 Chen-Chi M Ma,3 Yu-Jen Lu,4 Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; 2Animal Molecular Imaging Center and Department of Nuclear Medicine, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; 3Department of Chemical Engineering, National Tsing Hua University, Hsin-Chu, Taiwan; 4Department of Neurosurgery, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan *These authors contributed equally to this work Background: Graphene oxide (GO) is a promising nanomaterial for potential application in the versatile field of biomedicine. Graphene-based nanomaterials have been reported to modulate the functionality of immune cells in culture and to induce pulmonary inflammation in mice. Evidence pertaining to the interaction between graphene-based nanomaterials and the immune system in vivo remains scarce. The present study investigated the effect of polyethylene glycol-coated GO (PEG-GO) on antigen-specific immunity in vivo. Methods: BALB/c mice were intravenously administered with a single dose of PEG-GO (0.5 or 1 mg/kg) 1 hour before ovalbumin (OVA) sensitization, and antigen-specific antibody production and splenocyte reactivity were measured 7 days later. Results: Exposure to PEG-GO significantly attenuated the serum level of OVA-specific immunoglobulin E. The production of interferon-γ and interleukin-4 by splenocytes restimulated with OVA in culture was enhanced by treatment with PEG-GO. In addition, PEG-GO augmented the metabolic activity of splenocytes restimulated with OVA but not with the T-cell mitogen concanavalin A. Conclusion: Collectively, these results demonstrate that systemic exposure to PEG-GO modulates several aspects of antigen-specific immune responses, including the serum production of immunoglobulin E and T-cell functionality. Keywords: graphene oxide, T-cell, antigen-specific, immune, ovalbuminWu HYLin KJWang PYLin CWYang HWMa CCLu YJJan TRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 4257-4266 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wu HY Lin KJ Wang PY Lin CW Yang HW Ma CC Lu YJ Jan TR Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
description	Hsin-Ying Wu,1,* Kun-Ju Lin,2,* Ping-Yen Wang,1 Chi-Wen Lin,3 Hong-Wei Yang,3 Chen-Chi M Ma,3 Yu-Jen Lu,4 Tong-Rong Jan1 1Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; 2Animal Molecular Imaging Center and Department of Nuclear Medicine, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; 3Department of Chemical Engineering, National Tsing Hua University, Hsin-Chu, Taiwan; 4Department of Neurosurgery, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan *These authors contributed equally to this work Background: Graphene oxide (GO) is a promising nanomaterial for potential application in the versatile field of biomedicine. Graphene-based nanomaterials have been reported to modulate the functionality of immune cells in culture and to induce pulmonary inflammation in mice. Evidence pertaining to the interaction between graphene-based nanomaterials and the immune system in vivo remains scarce. The present study investigated the effect of polyethylene glycol-coated GO (PEG-GO) on antigen-specific immunity in vivo. Methods: BALB/c mice were intravenously administered with a single dose of PEG-GO (0.5 or 1 mg/kg) 1 hour before ovalbumin (OVA) sensitization, and antigen-specific antibody production and splenocyte reactivity were measured 7 days later. Results: Exposure to PEG-GO significantly attenuated the serum level of OVA-specific immunoglobulin E. The production of interferon-γ and interleukin-4 by splenocytes restimulated with OVA in culture was enhanced by treatment with PEG-GO. In addition, PEG-GO augmented the metabolic activity of splenocytes restimulated with OVA but not with the T-cell mitogen concanavalin A. Conclusion: Collectively, these results demonstrate that systemic exposure to PEG-GO modulates several aspects of antigen-specific immune responses, including the serum production of immunoglobulin E and T-cell functionality. Keywords: graphene oxide, T-cell, antigen-specific, immune, ovalbumin
format	article
author	Wu HY Lin KJ Wang PY Lin CW Yang HW Ma CC Lu YJ Jan TR
author_facet	Wu HY Lin KJ Wang PY Lin CW Yang HW Ma CC Lu YJ Jan TR
author_sort	Wu HY
title	Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_short	Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_full	Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_fullStr	Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_full_unstemmed	Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
title_sort	polyethylene glycol-coated graphene oxide attenuates antigen-specific ige production and enhanced antigen-induced t-cell reactivity in ovalbumin-sensitized balb/c mice
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e6db9041fcef45cc9d0a9541bf00b56d
work_keys_str_mv	AT wuhy polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT linkj polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT wangpy polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT lincw polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT yanghw polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT macc polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT luyj polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice AT jantr polyethyleneglycolcoatedgrapheneoxideattenuatesantigenspecificigeproductionandenhancedantigeninducedtcellreactivityinovalbuminsensitizedbalbcmice
_version_	1718398634946461696

Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice

Ejemplares similares