PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses

The tiger-fly Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identified its preferred prey, but a more in-dep...

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Autores principales:	Sofia G. SEABRA, Joana MARTINS, Patrícia BRÁS, Ana M. TAVARES, Inês FREITAS, António BARATA, Maria Teresa REBELO, Célia MATEUS, Octávio S. PAULO, Elisabete FIGUEIREDO
Formato:	article
Lenguaje:	EN
Publicado:	Institute of Entomology, Biology Centre, Czech Academy of Science 2021
Materias:	diptera muscidae coenosia attenuata predation gut contents prey molecular identification polymerase chain reaction probit models half-time molecular detection Zoology QL1-991
Acceso en línea:	https://doaj.org/article/e6e49604efd74e1c881395df90da680a
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spelling	oai:doaj.org-article:e6e49604efd74e1c881395df90da680a2021-11-05T15:23:15ZPCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses1210-57591802-882910.14411/eje.2021.035https://doaj.org/article/e6e49604efd74e1c881395df90da680a2021-10-01T00:00:00Zhttps://www.eje.cz/artkey/eje-202101-0035_pcr-based_detection_of_prey_dna_in_the_gut_contents_of_the_tiger-fly_coenosia_attenuata_diptera_muscidae_a.phphttps://doaj.org/toc/1210-5759https://doaj.org/toc/1802-8829The tiger-fly Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identified its preferred prey, but a more in-depth evaluation will benefit from using Polymerase Chain Reaction amplification of prey DNA remains in the gut of this predator. To evaluate the rate of decay and suitability of this method for use in the field assessments, we carried out a laboratory feeding calibration experiment on 355 females of C. attenuata, which were killed at different intervals of time after ingestion (10 time points from 0 to 48 h). The prey species tested were: Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae: Trialeurodini), Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), Diglyphus isaea (Walker) (Hymenoptera: Eulophidae: Cirrospilini), Bradysia impatiens (Johannsen) (Diptera: Sciaridae) and Drosophila mercatorum Patterson & Wheeler (Diptera: Drosophilidae: Drosophilini). Based on a probit model, amplification success of prey DNA declined exponentially with increasing time after ingestion. The half-time molecular detection differed between species, ranging from an average of 5 h for T. vaporariorum and D. isaea, 6 h for B. impatiens, 15 h for L. huidobrensis to more than 40 h for D. mercatorum. This study confirmed the feasibility of using DNA based detection to identify prey species in the gut contents of C. attenuata and provided calibration curves for a better understanding of predation activity in this agroecosystem.Sofia G. SEABRAJoana MARTINSPatrícia BRÁSAna M. TAVARESInês FREITASAntónio BARATAMaria Teresa REBELOCélia MATEUSOctávio S. PAULOElisabete FIGUEIREDOInstitute of Entomology, Biology Centre, Czech Academy of Sciencearticledipteramuscidaecoenosia attenuatapredationgut contentsprey molecular identificationpolymerase chain reactionprobit modelshalf-time molecular detectionZoologyQL1-991ENEuropean Journal of Entomology, Vol 118, Iss 1, Pp 335-343 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diptera muscidae coenosia attenuata predation gut contents prey molecular identification polymerase chain reaction probit models half-time molecular detection Zoology QL1-991
spellingShingle	diptera muscidae coenosia attenuata predation gut contents prey molecular identification polymerase chain reaction probit models half-time molecular detection Zoology QL1-991 Sofia G. SEABRA Joana MARTINS Patrícia BRÁS Ana M. TAVARES Inês FREITAS António BARATA Maria Teresa REBELO Célia MATEUS Octávio S. PAULO Elisabete FIGUEIREDO PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
description	The tiger-fly Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identified its preferred prey, but a more in-depth evaluation will benefit from using Polymerase Chain Reaction amplification of prey DNA remains in the gut of this predator. To evaluate the rate of decay and suitability of this method for use in the field assessments, we carried out a laboratory feeding calibration experiment on 355 females of C. attenuata, which were killed at different intervals of time after ingestion (10 time points from 0 to 48 h). The prey species tested were: Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae: Trialeurodini), Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), Diglyphus isaea (Walker) (Hymenoptera: Eulophidae: Cirrospilini), Bradysia impatiens (Johannsen) (Diptera: Sciaridae) and Drosophila mercatorum Patterson & Wheeler (Diptera: Drosophilidae: Drosophilini). Based on a probit model, amplification success of prey DNA declined exponentially with increasing time after ingestion. The half-time molecular detection differed between species, ranging from an average of 5 h for T. vaporariorum and D. isaea, 6 h for B. impatiens, 15 h for L. huidobrensis to more than 40 h for D. mercatorum. This study confirmed the feasibility of using DNA based detection to identify prey species in the gut contents of C. attenuata and provided calibration curves for a better understanding of predation activity in this agroecosystem.
format	article
author	Sofia G. SEABRA Joana MARTINS Patrícia BRÁS Ana M. TAVARES Inês FREITAS António BARATA Maria Teresa REBELO Célia MATEUS Octávio S. PAULO Elisabete FIGUEIREDO
author_facet	Sofia G. SEABRA Joana MARTINS Patrícia BRÁS Ana M. TAVARES Inês FREITAS António BARATA Maria Teresa REBELO Célia MATEUS Octávio S. PAULO Elisabete FIGUEIREDO
author_sort	Sofia G. SEABRA
title	PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_short	PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_full	PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_fullStr	PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_full_unstemmed	PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
title_sort	pcr-based detection of prey dna in the gut contents of the tiger-fly, coenosia attenuata (diptera: muscidae), a biological control agent in mediterranean greenhouses
publisher	Institute of Entomology, Biology Centre, Czech Academy of Science
publishDate	2021
url	https://doaj.org/article/e6e49604efd74e1c881395df90da680a
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PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses

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