Engineering of CRISPR-Cas12b for human genome editing

The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.

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Autores principales:	Jonathan Strecker, Sara Jones, Balwina Koopal, Jonathan Schmid-Burgk, Bernd Zetsche, Linyi Gao, Kira S. Makarova, Eugene V. Koonin, Feng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e6ea12311957487294bb0381d2fecb20
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spelling	oai:doaj.org-article:e6ea12311957487294bb0381d2fecb202021-12-02T15:35:06ZEngineering of CRISPR-Cas12b for human genome editing10.1038/s41467-018-08224-42041-1723https://doaj.org/article/e6ea12311957487294bb0381d2fecb202019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08224-4https://doaj.org/toc/2041-1723The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.Jonathan StreckerSara JonesBalwina KoopalJonathan Schmid-BurgkBernd ZetscheLinyi GaoKira S. MakarovaEugene V. KooninFeng ZhangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jonathan Strecker Sara Jones Balwina Koopal Jonathan Schmid-Burgk Bernd Zetsche Linyi Gao Kira S. Makarova Eugene V. Koonin Feng Zhang Engineering of CRISPR-Cas12b for human genome editing
description	The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.
format	article
author	Jonathan Strecker Sara Jones Balwina Koopal Jonathan Schmid-Burgk Bernd Zetsche Linyi Gao Kira S. Makarova Eugene V. Koonin Feng Zhang
author_facet	Jonathan Strecker Sara Jones Balwina Koopal Jonathan Schmid-Burgk Bernd Zetsche Linyi Gao Kira S. Makarova Eugene V. Koonin Feng Zhang
author_sort	Jonathan Strecker
title	Engineering of CRISPR-Cas12b for human genome editing
title_short	Engineering of CRISPR-Cas12b for human genome editing
title_full	Engineering of CRISPR-Cas12b for human genome editing
title_fullStr	Engineering of CRISPR-Cas12b for human genome editing
title_full_unstemmed	Engineering of CRISPR-Cas12b for human genome editing
title_sort	engineering of crispr-cas12b for human genome editing
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e6ea12311957487294bb0381d2fecb20
work_keys_str_mv	AT jonathanstrecker engineeringofcrisprcas12bforhumangenomeediting AT sarajones engineeringofcrisprcas12bforhumangenomeediting AT balwinakoopal engineeringofcrisprcas12bforhumangenomeediting AT jonathanschmidburgk engineeringofcrisprcas12bforhumangenomeediting AT berndzetsche engineeringofcrisprcas12bforhumangenomeediting AT linyigao engineeringofcrisprcas12bforhumangenomeediting AT kirasmakarova engineeringofcrisprcas12bforhumangenomeediting AT eugenevkoonin engineeringofcrisprcas12bforhumangenomeediting AT fengzhang engineeringofcrisprcas12bforhumangenomeediting
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Engineering of CRISPR-Cas12b for human genome editing

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