Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus

Abstract Fish regulate ventilation primarily by sensing O2-levels in the water and arterial blood. It is well established that this sensory process involves several steps, but the underlying mechanisms remain frustratingly elusive. Here we examine the effect of increasing lactate ions at constant pH...

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Autores principales:	Mikkel T. Thomsen, Tobias Wang, William K. Milsom, Mark Bayley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6ec6bb51e0e4ec28d5392c7a2034863
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spelling	oai:doaj.org-article:e6ec6bb51e0e4ec28d5392c7a20348632021-12-02T15:06:22ZLactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus10.1038/s41598-017-06745-42045-2322https://doaj.org/article/e6ec6bb51e0e4ec28d5392c7a20348632017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06745-4https://doaj.org/toc/2045-2322Abstract Fish regulate ventilation primarily by sensing O2-levels in the water and arterial blood. It is well established that this sensory process involves several steps, but the underlying mechanisms remain frustratingly elusive. Here we examine the effect of increasing lactate ions at constant pH on ventilation in a teleost; specifically the facultative air-breathing catfish Pangasianodon hypophthalmus. At lactate levels within the physiological range obtained by Na-Lactate injections (3.5 ± 0.8 to 10.9 ± 0.7 mmol L−1), gill ventilation increased in a dose-dependent manner to levels comparable to those elicited by NaCN injections (2.0 µmol kg−1), which induces a hypoxic response and higher than those observed in any level of ambient hypoxia (lowest PO2 = 20 mmHg). High lactate concentrations also stimulated air-breathing. Denervation of the first gill arch reduced the ventilatory response to lactate suggesting that part of the sensory mechanism for lactate is located at the first gill arch. However, since a residual response remained after this denervation, the other gill arches or extrabranchial locations must also be important for lactate sensing. We propose that lactate plays a role as a signalling molecule in the hypoxic ventilatory response in fish.Mikkel T. ThomsenTobias WangWilliam K. MilsomMark BayleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mikkel T. Thomsen Tobias Wang William K. Milsom Mark Bayley Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
description	Abstract Fish regulate ventilation primarily by sensing O2-levels in the water and arterial blood. It is well established that this sensory process involves several steps, but the underlying mechanisms remain frustratingly elusive. Here we examine the effect of increasing lactate ions at constant pH on ventilation in a teleost; specifically the facultative air-breathing catfish Pangasianodon hypophthalmus. At lactate levels within the physiological range obtained by Na-Lactate injections (3.5 ± 0.8 to 10.9 ± 0.7 mmol L−1), gill ventilation increased in a dose-dependent manner to levels comparable to those elicited by NaCN injections (2.0 µmol kg−1), which induces a hypoxic response and higher than those observed in any level of ambient hypoxia (lowest PO2 = 20 mmHg). High lactate concentrations also stimulated air-breathing. Denervation of the first gill arch reduced the ventilatory response to lactate suggesting that part of the sensory mechanism for lactate is located at the first gill arch. However, since a residual response remained after this denervation, the other gill arches or extrabranchial locations must also be important for lactate sensing. We propose that lactate plays a role as a signalling molecule in the hypoxic ventilatory response in fish.
format	article
author	Mikkel T. Thomsen Tobias Wang William K. Milsom Mark Bayley
author_facet	Mikkel T. Thomsen Tobias Wang William K. Milsom Mark Bayley
author_sort	Mikkel T. Thomsen
title	Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
title_short	Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
title_full	Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
title_fullStr	Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
title_full_unstemmed	Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
title_sort	lactate provides a strong ph-independent ventilatory signal in the facultative air-breathing teleost pangasianodon hypophthalmus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e6ec6bb51e0e4ec28d5392c7a2034863
work_keys_str_mv	AT mikkeltthomsen lactateprovidesastrongphindependentventilatorysignalinthefacultativeairbreathingteleostpangasianodonhypophthalmus AT tobiaswang lactateprovidesastrongphindependentventilatorysignalinthefacultativeairbreathingteleostpangasianodonhypophthalmus AT williamkmilsom lactateprovidesastrongphindependentventilatorysignalinthefacultativeairbreathingteleostpangasianodonhypophthalmus AT markbayley lactateprovidesastrongphindependentventilatorysignalinthefacultativeairbreathingteleostpangasianodonhypophthalmus
_version_	1718388485865340928

Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus

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