The untranslated regions of classic swine fever virus RNA trigger apoptosis.

Classical swine fever virus (CSFV) causes a broad range of disease in pigs, from acute symptoms including high fever and hemorrhages, to chronic disease or unapparent infection, depending on the virus strain. CSFV belongs to the genus Pestivirus of the family Flaviviridae. It carries a single-strand...

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Autores principales:	Wei-Li Hsu, Chung-Lun Chen, Shi-Wei Huang, Chia-Chen Wu, I-Hsuan Chen, Muthukumar Nadar, Yin-Peng Su, Ching-Hsiu Tsai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6f249ff79544cf9aefe0343ca430f53
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spelling	oai:doaj.org-article:e6f249ff79544cf9aefe0343ca430f532021-11-18T08:32:44ZThe untranslated regions of classic swine fever virus RNA trigger apoptosis.1932-620310.1371/journal.pone.0088863https://doaj.org/article/e6f249ff79544cf9aefe0343ca430f532014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24533157/?tool=EBIhttps://doaj.org/toc/1932-6203Classical swine fever virus (CSFV) causes a broad range of disease in pigs, from acute symptoms including high fever and hemorrhages, to chronic disease or unapparent infection, depending on the virus strain. CSFV belongs to the genus Pestivirus of the family Flaviviridae. It carries a single-stranded positive-sense RNA genome. An internal ribosomal entry site (IRES) in the 5' untranslated region (UTR) drives the translation of a single open reading frame encoding a 3898 amino acid long polypeptide chain. The open reading frame is followed by a 3' UTR comprising four highly structured stem-loops. In the present study, a synthetic RNA composed of the 5' and 3' UTRs of the CSFV genome devoid of any viral coding sequence and separated by a luciferase gene cassette (designated 5'UTR-Luc-3'UTR) triggered apoptotic cell death as early as 4 h post-transfection. The apoptosis was measured by DNA laddering analysis, TUNEL assay, annexin-V binding determined by flow cytometry, and by analysis of caspase activation. Contrasting with this, only trace DNA laddering was observed in cells transfected with the individual 5' or 3' UTR RNA; even when the 5' UTR and 3' UTR were co-transfected as separate RNA molecules, DNA laddering did not reach the level induced by the chimeric 5'UTR-Luc-3'UTR RNA. Interestingly, RNA composed of the 5'UTR and of stem-loop I of the 3'UTR triggered much stronger apoptosis than the 5' or 3'UTR alone. These results indicate that the 5' and 3' UTRs act together in cis induce apoptosis. We furthered obtained evidence that the UTR-mediated apoptosis required double-stranded RNA and involved translation shutoff possibly through activation of PKR.Wei-Li HsuChung-Lun ChenShi-Wei HuangChia-Chen WuI-Hsuan ChenMuthukumar NadarYin-Peng SuChing-Hsiu TsaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88863 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Li Hsu Chung-Lun Chen Shi-Wei Huang Chia-Chen Wu I-Hsuan Chen Muthukumar Nadar Yin-Peng Su Ching-Hsiu Tsai The untranslated regions of classic swine fever virus RNA trigger apoptosis.
description	Classical swine fever virus (CSFV) causes a broad range of disease in pigs, from acute symptoms including high fever and hemorrhages, to chronic disease or unapparent infection, depending on the virus strain. CSFV belongs to the genus Pestivirus of the family Flaviviridae. It carries a single-stranded positive-sense RNA genome. An internal ribosomal entry site (IRES) in the 5' untranslated region (UTR) drives the translation of a single open reading frame encoding a 3898 amino acid long polypeptide chain. The open reading frame is followed by a 3' UTR comprising four highly structured stem-loops. In the present study, a synthetic RNA composed of the 5' and 3' UTRs of the CSFV genome devoid of any viral coding sequence and separated by a luciferase gene cassette (designated 5'UTR-Luc-3'UTR) triggered apoptotic cell death as early as 4 h post-transfection. The apoptosis was measured by DNA laddering analysis, TUNEL assay, annexin-V binding determined by flow cytometry, and by analysis of caspase activation. Contrasting with this, only trace DNA laddering was observed in cells transfected with the individual 5' or 3' UTR RNA; even when the 5' UTR and 3' UTR were co-transfected as separate RNA molecules, DNA laddering did not reach the level induced by the chimeric 5'UTR-Luc-3'UTR RNA. Interestingly, RNA composed of the 5'UTR and of stem-loop I of the 3'UTR triggered much stronger apoptosis than the 5' or 3'UTR alone. These results indicate that the 5' and 3' UTRs act together in cis induce apoptosis. We furthered obtained evidence that the UTR-mediated apoptosis required double-stranded RNA and involved translation shutoff possibly through activation of PKR.
format	article
author	Wei-Li Hsu Chung-Lun Chen Shi-Wei Huang Chia-Chen Wu I-Hsuan Chen Muthukumar Nadar Yin-Peng Su Ching-Hsiu Tsai
author_facet	Wei-Li Hsu Chung-Lun Chen Shi-Wei Huang Chia-Chen Wu I-Hsuan Chen Muthukumar Nadar Yin-Peng Su Ching-Hsiu Tsai
author_sort	Wei-Li Hsu
title	The untranslated regions of classic swine fever virus RNA trigger apoptosis.
title_short	The untranslated regions of classic swine fever virus RNA trigger apoptosis.
title_full	The untranslated regions of classic swine fever virus RNA trigger apoptosis.
title_fullStr	The untranslated regions of classic swine fever virus RNA trigger apoptosis.
title_full_unstemmed	The untranslated regions of classic swine fever virus RNA trigger apoptosis.
title_sort	untranslated regions of classic swine fever virus rna trigger apoptosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e6f249ff79544cf9aefe0343ca430f53
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The untranslated regions of classic swine fever virus RNA trigger apoptosis.

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