Highly active enzymes by automated combinatorial backbone assembly and sequence design

Computationally designed enzymes often show lower activity or stability than their natural counterparts. Here, the authors present an evolution-inspired method for automated enzyme design, creating stable enzymes with accurate active site architectures and wild-type-like activities.

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Autores principales:	Gideon Lapidoth, Olga Khersonsky, Rosalie Lipsh, Orly Dym, Shira Albeck, Shelly Rogotner, Sarel J. Fleishman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e6f62792433646fab21959e1b02b0675
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spelling	oai:doaj.org-article:e6f62792433646fab21959e1b02b06752021-12-02T17:32:16ZHighly active enzymes by automated combinatorial backbone assembly and sequence design10.1038/s41467-018-05205-52041-1723https://doaj.org/article/e6f62792433646fab21959e1b02b06752018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05205-5https://doaj.org/toc/2041-1723Computationally designed enzymes often show lower activity or stability than their natural counterparts. Here, the authors present an evolution-inspired method for automated enzyme design, creating stable enzymes with accurate active site architectures and wild-type-like activities.Gideon LapidothOlga KhersonskyRosalie LipshOrly DymShira AlbeckShelly RogotnerSarel J. FleishmanNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gideon Lapidoth Olga Khersonsky Rosalie Lipsh Orly Dym Shira Albeck Shelly Rogotner Sarel J. Fleishman Highly active enzymes by automated combinatorial backbone assembly and sequence design
description	Computationally designed enzymes often show lower activity or stability than their natural counterparts. Here, the authors present an evolution-inspired method for automated enzyme design, creating stable enzymes with accurate active site architectures and wild-type-like activities.
format	article
author	Gideon Lapidoth Olga Khersonsky Rosalie Lipsh Orly Dym Shira Albeck Shelly Rogotner Sarel J. Fleishman
author_facet	Gideon Lapidoth Olga Khersonsky Rosalie Lipsh Orly Dym Shira Albeck Shelly Rogotner Sarel J. Fleishman
author_sort	Gideon Lapidoth
title	Highly active enzymes by automated combinatorial backbone assembly and sequence design
title_short	Highly active enzymes by automated combinatorial backbone assembly and sequence design
title_full	Highly active enzymes by automated combinatorial backbone assembly and sequence design
title_fullStr	Highly active enzymes by automated combinatorial backbone assembly and sequence design
title_full_unstemmed	Highly active enzymes by automated combinatorial backbone assembly and sequence design
title_sort	highly active enzymes by automated combinatorial backbone assembly and sequence design
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e6f62792433646fab21959e1b02b0675
work_keys_str_mv	AT gideonlapidoth highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT olgakhersonsky highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT rosalielipsh highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT orlydym highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT shiraalbeck highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT shellyrogotner highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign AT sareljfleishman highlyactiveenzymesbyautomatedcombinatorialbackboneassemblyandsequencedesign
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Highly active enzymes by automated combinatorial backbone assembly and sequence design

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