Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.

Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non-cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plan...

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Autores principales:	Carole L Thomas, Emmanuelle M Bayer, Christophe Ritzenthaler, Lourdes Fernandez-Calvino, Andrew J Maule
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e6fd29a6b301483e9b33e6812a36dcd5
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spelling	oai:doaj.org-article:e6fd29a6b301483e9b33e6812a36dcd52021-11-25T05:33:32ZSpecific targeting of a plasmodesmal protein affecting cell-to-cell communication.1544-91731545-788510.1371/journal.pbio.0060007https://doaj.org/article/e6fd29a6b301483e9b33e6812a36dcd52008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18215111/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non-cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plant biology. Features of protein sequences and/or structure that determine protein targeting to plasmodesmata were previously unknown. We identify here a novel family of plasmodesmata-located proteins (called PDLP1) whose members have the features of type I membrane receptor-like proteins. We focus our studies on the first identified type member (namely At5g43980, or PDLP1a) and show that, following its altered expression, it is effective in modulating cell-to-cell trafficking. PDLP1a is targeted to plasmodesmata via the secretory pathway in a Brefeldin A-sensitive and COPII-dependent manner, and resides at plasmodesmata with its C-terminus in the cytoplasmic domain and its N-terminus in the apoplast. Using a deletion analysis, we show that the single transmembrane domain (TMD) of PDLP1a contains all the information necessary for intracellular targeting of this type I membrane protein to plasmodesmata, such that the TMD can be used to target heterologous proteins to this location. These studies identify a new family of plasmodesmal proteins that affect cell-to-cell communication. They exhibit a mode of intracellular trafficking and targeting novel for plant biology and provide technological opportunities for targeting different proteins to plasmodesmata to aid in plasmodesmal characterisation.Carole L ThomasEmmanuelle M BayerChristophe RitzenthalerLourdes Fernandez-CalvinoAndrew J MaulePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 1, p e7 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Carole L Thomas Emmanuelle M Bayer Christophe Ritzenthaler Lourdes Fernandez-Calvino Andrew J Maule Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
description	Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non-cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plant biology. Features of protein sequences and/or structure that determine protein targeting to plasmodesmata were previously unknown. We identify here a novel family of plasmodesmata-located proteins (called PDLP1) whose members have the features of type I membrane receptor-like proteins. We focus our studies on the first identified type member (namely At5g43980, or PDLP1a) and show that, following its altered expression, it is effective in modulating cell-to-cell trafficking. PDLP1a is targeted to plasmodesmata via the secretory pathway in a Brefeldin A-sensitive and COPII-dependent manner, and resides at plasmodesmata with its C-terminus in the cytoplasmic domain and its N-terminus in the apoplast. Using a deletion analysis, we show that the single transmembrane domain (TMD) of PDLP1a contains all the information necessary for intracellular targeting of this type I membrane protein to plasmodesmata, such that the TMD can be used to target heterologous proteins to this location. These studies identify a new family of plasmodesmal proteins that affect cell-to-cell communication. They exhibit a mode of intracellular trafficking and targeting novel for plant biology and provide technological opportunities for targeting different proteins to plasmodesmata to aid in plasmodesmal characterisation.
format	article
author	Carole L Thomas Emmanuelle M Bayer Christophe Ritzenthaler Lourdes Fernandez-Calvino Andrew J Maule
author_facet	Carole L Thomas Emmanuelle M Bayer Christophe Ritzenthaler Lourdes Fernandez-Calvino Andrew J Maule
author_sort	Carole L Thomas
title	Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
title_short	Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
title_full	Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
title_fullStr	Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
title_full_unstemmed	Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
title_sort	specific targeting of a plasmodesmal protein affecting cell-to-cell communication.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/e6fd29a6b301483e9b33e6812a36dcd5
work_keys_str_mv	AT carolelthomas specifictargetingofaplasmodesmalproteinaffectingcelltocellcommunication AT emmanuellembayer specifictargetingofaplasmodesmalproteinaffectingcelltocellcommunication AT christopheritzenthaler specifictargetingofaplasmodesmalproteinaffectingcelltocellcommunication AT lourdesfernandezcalvino specifictargetingofaplasmodesmalproteinaffectingcelltocellcommunication AT andrewjmaule specifictargetingofaplasmodesmalproteinaffectingcelltocellcommunication
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Specific targeting of a plasmodesmal protein affecting cell-to-cell communication.

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