STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes

Abstract Stromal interaction molecule 1 (STIM1) along with Orai1 mediates extracellular Ca2+ entry into the cytosol through a store-operated Ca2+ entry (SOCE) mechanism in various tissues including skeletal muscle. However, the role(s) of STIM2, a homolog of STIM1, in skeletal muscle has not been we...

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Autores principales:	Mi Ri Oh, Keon Jin Lee, Mei Huang, Jin Ock Kim, Do Han Kim, Chung-Hyun Cho, Eun Hui Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6fd67e21a754d228de971432b7c3e58
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spelling	oai:doaj.org-article:e6fd67e21a754d228de971432b7c3e582021-12-02T15:05:52ZSTIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes10.1038/s41598-017-18256-32045-2322https://doaj.org/article/e6fd67e21a754d228de971432b7c3e582017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18256-3https://doaj.org/toc/2045-2322Abstract Stromal interaction molecule 1 (STIM1) along with Orai1 mediates extracellular Ca2+ entry into the cytosol through a store-operated Ca2+ entry (SOCE) mechanism in various tissues including skeletal muscle. However, the role(s) of STIM2, a homolog of STIM1, in skeletal muscle has not been well addressed. The present study, first, was focused on searching for STIM2-binding proteins from among proteins mediating skeletal muscle functions. This study used a binding assay, quadrupole time-of-flight mass spectrometry, and co-immunoprecipitation assay with bona-fide STIM2- and SERCA1a-expressing rabbit skeletal muscle. The region for amino acids from 453 to 729 of STIM2 binds to sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 1a (SERCA1a). Next, oxalate-supported 45Ca2+-uptake experiments and various single-myotube Ca2+ imaging experiments using STIM2-knockdown mouse primary skeletal myotubes have suggested that STIM2 attenuates SERCA1a activity during skeletal muscle contraction, which contributes to the intracellular Ca2+ distribution between the cytosol and the SR at rest. In addition, STIM2 regulates Ca2+ movement through RyR1 during skeletal muscle contraction as well as SOCE. Therefore, via regulation of SERCA1a activity, STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal muscle, which makes it both similar to, yet different from, STIM1.Mi Ri OhKeon Jin LeeMei HuangJin Ock KimDo Han KimChung-Hyun ChoEun Hui LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mi Ri Oh Keon Jin Lee Mei Huang Jin Ock Kim Do Han Kim Chung-Hyun Cho Eun Hui Lee STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
description	Abstract Stromal interaction molecule 1 (STIM1) along with Orai1 mediates extracellular Ca2+ entry into the cytosol through a store-operated Ca2+ entry (SOCE) mechanism in various tissues including skeletal muscle. However, the role(s) of STIM2, a homolog of STIM1, in skeletal muscle has not been well addressed. The present study, first, was focused on searching for STIM2-binding proteins from among proteins mediating skeletal muscle functions. This study used a binding assay, quadrupole time-of-flight mass spectrometry, and co-immunoprecipitation assay with bona-fide STIM2- and SERCA1a-expressing rabbit skeletal muscle. The region for amino acids from 453 to 729 of STIM2 binds to sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 1a (SERCA1a). Next, oxalate-supported 45Ca2+-uptake experiments and various single-myotube Ca2+ imaging experiments using STIM2-knockdown mouse primary skeletal myotubes have suggested that STIM2 attenuates SERCA1a activity during skeletal muscle contraction, which contributes to the intracellular Ca2+ distribution between the cytosol and the SR at rest. In addition, STIM2 regulates Ca2+ movement through RyR1 during skeletal muscle contraction as well as SOCE. Therefore, via regulation of SERCA1a activity, STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal muscle, which makes it both similar to, yet different from, STIM1.
format	article
author	Mi Ri Oh Keon Jin Lee Mei Huang Jin Ock Kim Do Han Kim Chung-Hyun Cho Eun Hui Lee
author_facet	Mi Ri Oh Keon Jin Lee Mei Huang Jin Ock Kim Do Han Kim Chung-Hyun Cho Eun Hui Lee
author_sort	Mi Ri Oh
title	STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
title_short	STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
title_full	STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
title_fullStr	STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
title_full_unstemmed	STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
title_sort	stim2 regulates both intracellular ca2+ distribution and ca2+ movement in skeletal myotubes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e6fd67e21a754d228de971432b7c3e58
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STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes

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