Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to...

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Autores principales: Liping Hong, Mingfei Pan, Xiaoqian Xie, Kaixin Liu, Jingying Yang, Shan Wang, Shuo Wang
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/e7026593907746c1bf18de3408211457
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spelling oai:doaj.org-article:e7026593907746c1bf18de34082114572021-11-25T17:33:11ZAptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices10.3390/foods101125982304-8158https://doaj.org/article/e7026593907746c1bf18de34082114572021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2598https://doaj.org/toc/2304-8158Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.Liping HongMingfei PanXiaoqian XieKaixin LiuJingying YangShan WangShuo WangMDPI AGarticleallergendetectionaptamerfluorescencefood safetyChemical technologyTP1-1185ENFoods, Vol 10, Iss 2598, p 2598 (2021)
institution DOAJ
collection DOAJ
language EN
topic allergen
detection
aptamer
fluorescence
food safety
Chemical technology
TP1-1185
spellingShingle allergen
detection
aptamer
fluorescence
food safety
Chemical technology
TP1-1185
Liping Hong
Mingfei Pan
Xiaoqian Xie
Kaixin Liu
Jingying Yang
Shan Wang
Shuo Wang
Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
description Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.
format article
author Liping Hong
Mingfei Pan
Xiaoqian Xie
Kaixin Liu
Jingying Yang
Shan Wang
Shuo Wang
author_facet Liping Hong
Mingfei Pan
Xiaoqian Xie
Kaixin Liu
Jingying Yang
Shan Wang
Shuo Wang
author_sort Liping Hong
title Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
title_short Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
title_full Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
title_fullStr Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
title_full_unstemmed Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices
title_sort aptamer-based fluorescent biosensor for the rapid and sensitive detection of allergens in food matrices
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/e7026593907746c1bf18de3408211457
work_keys_str_mv AT lipinghong aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT mingfeipan aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT xiaoqianxie aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT kaixinliu aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT jingyingyang aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT shanwang aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
AT shuowang aptamerbasedfluorescentbiosensorfortherapidandsensitivedetectionofallergensinfoodmatrices
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