Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development

Macrophages play a crucial role in the initiation and progression of rheumatoid arthritis (RA). Liposomes can be used to deliver therapeutics to macrophages by exploiting their phagocytic ability. However, since macrophages serve as the immune system’s first responders, it is inadvisable to systemic...

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Autores principales:	Daphne N. Dorst, Marti Boss, Mark Rijpkema, Birgitte Walgreen, Monique M. A. Helsen, Desirée L. Bos, Louis van Bloois, Gerrit Storm, Maarten Brom, Peter Laverman, Peter M. van der Kraan, Mijke Buitinga, Marije I. Koenders, Martin Gotthardt
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	photodynamic therapy liposomes experimental arthritis Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/e70353bcaa8c4f038122e08e45552458
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spelling	oai:doaj.org-article:e70353bcaa8c4f038122e08e455524582021-11-25T18:41:18ZPhotodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development10.3390/pharmaceutics131118681999-4923https://doaj.org/article/e70353bcaa8c4f038122e08e455524582021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1868https://doaj.org/toc/1999-4923Macrophages play a crucial role in the initiation and progression of rheumatoid arthritis (RA). Liposomes can be used to deliver therapeutics to macrophages by exploiting their phagocytic ability. However, since macrophages serve as the immune system’s first responders, it is inadvisable to systemically deplete these cells. By loading the liposomes with the photosensitizer IRDye700DX, we have developed and tested a novel way to perform photodynamic therapy (PDT) on macrophages in inflamed joints. PEGylated liposomes were created using the film method and post-inserted with micelles containing IRDye700DX. For radiolabeling, a chelator was also incorporated. RAW 264.7 cells were incubated with liposomes with or without IRDye700DX and exposed to 689 nm light. Viability was determined using CellTiterGlo. Subsequently, biodistribution and PDT studies were performed on mice with collagen-induced arthritis (CIA). PDT using IRDye700DX-loaded liposomes efficiently induced cell death in vitro, whilst no cell death was observed using the control liposomes. Biodistribution of the two compounds in CIA mice was comparable with excellent correlation of the uptake with macroscopic and microscopic arthritis scores. Treatment with 700DX-loaded liposomes significantly delayed arthritis development. Here we have shown the proof-of-principle of performing PDT in arthritic joints using IRDye700DX-loaded liposomes, allowing locoregional treatment of arthritis.Daphne N. DorstMarti BossMark RijpkemaBirgitte WalgreenMonique M. A. HelsenDesirée L. BosLouis van BlooisGerrit StormMaarten BromPeter LavermanPeter M. van der KraanMijke BuitingaMarije I. KoendersMartin GotthardtMDPI AGarticlephotodynamic therapyliposomesexperimental arthritisPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1868, p 1868 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photodynamic therapy liposomes experimental arthritis Pharmacy and materia medica RS1-441
spellingShingle	photodynamic therapy liposomes experimental arthritis Pharmacy and materia medica RS1-441 Daphne N. Dorst Marti Boss Mark Rijpkema Birgitte Walgreen Monique M. A. Helsen Desirée L. Bos Louis van Bloois Gerrit Storm Maarten Brom Peter Laverman Peter M. van der Kraan Mijke Buitinga Marije I. Koenders Martin Gotthardt Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
description	Macrophages play a crucial role in the initiation and progression of rheumatoid arthritis (RA). Liposomes can be used to deliver therapeutics to macrophages by exploiting their phagocytic ability. However, since macrophages serve as the immune system’s first responders, it is inadvisable to systemically deplete these cells. By loading the liposomes with the photosensitizer IRDye700DX, we have developed and tested a novel way to perform photodynamic therapy (PDT) on macrophages in inflamed joints. PEGylated liposomes were created using the film method and post-inserted with micelles containing IRDye700DX. For radiolabeling, a chelator was also incorporated. RAW 264.7 cells were incubated with liposomes with or without IRDye700DX and exposed to 689 nm light. Viability was determined using CellTiterGlo. Subsequently, biodistribution and PDT studies were performed on mice with collagen-induced arthritis (CIA). PDT using IRDye700DX-loaded liposomes efficiently induced cell death in vitro, whilst no cell death was observed using the control liposomes. Biodistribution of the two compounds in CIA mice was comparable with excellent correlation of the uptake with macroscopic and microscopic arthritis scores. Treatment with 700DX-loaded liposomes significantly delayed arthritis development. Here we have shown the proof-of-principle of performing PDT in arthritic joints using IRDye700DX-loaded liposomes, allowing locoregional treatment of arthritis.
format	article
author	Daphne N. Dorst Marti Boss Mark Rijpkema Birgitte Walgreen Monique M. A. Helsen Desirée L. Bos Louis van Bloois Gerrit Storm Maarten Brom Peter Laverman Peter M. van der Kraan Mijke Buitinga Marije I. Koenders Martin Gotthardt
author_facet	Daphne N. Dorst Marti Boss Mark Rijpkema Birgitte Walgreen Monique M. A. Helsen Desirée L. Bos Louis van Bloois Gerrit Storm Maarten Brom Peter Laverman Peter M. van der Kraan Mijke Buitinga Marije I. Koenders Martin Gotthardt
author_sort	Daphne N. Dorst
title	Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
title_short	Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
title_full	Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
title_fullStr	Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
title_full_unstemmed	Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development
title_sort	photodynamic therapy targeting macrophages using irdye700dx-liposomes decreases experimental arthritis development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e70353bcaa8c4f038122e08e45552458
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Photodynamic Therapy Targeting Macrophages Using IRDye700DX-Liposomes Decreases Experimental Arthritis Development

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