Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches

Janatiningrum I, Solihin DD, Meryandini A, Lestari Y. 2018. Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches. Biodiversitas 19: 1514-1520. Actinobacteria endophytes of medicinal plants may play an esse...

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Autores principales:	ISRA JANATININGRUM, DEDY DURYADI SOLIHIN, ANJA MERYANDINI, YULIN LESTARI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	actinobacteria endophytes ficus deltoidea metagenomic pcr-dgge Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e7077481acd94f659e8ccbb2d8f82c48
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spelling	oai:doaj.org-article:e7077481acd94f659e8ccbb2d8f82c482021-11-16T14:03:29ZComparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches1412-033X2085-472210.13057/biodiv/d190443https://doaj.org/article/e7077481acd94f659e8ccbb2d8f82c482018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2823https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Janatiningrum I, Solihin DD, Meryandini A, Lestari Y. 2018. Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches. Biodiversitas 19: 1514-1520. Actinobacteria endophytes of medicinal plants may play an essential role in producing a variety of critical bioactive compounds. However, the possible contribution of such actinobacteria to the pharmacological properties of traditional herbal remedies remains mostly unknown. For example, the diversity and attributes of actinobacteria endophytes in Ficus deltoidea, a small tree species that has long been used to treat diseases such as cancer, diabetes, and cardiovascular illnesses, have not been explored. Here, the actinobacteria endophyte community structure in F. deltoidea was investigated using both culture-dependent and metagenomics approaches. Based on morphological characteristics and 16S rRNA gene analysis, the dominant culturable actinobacteria isolates exhibited a close relationship with Streptomyces. The metagenomic technique using PCR-DGGE analysis of the 16S rRNA gene showed the presence of 11 OTUs in F. deltoidea tissue. Whereas the dominant culturable actinobacteria endophytes in F. deltoidea was Streptomyces, the metagenomic approach showed non-Streptomyces, particularly Rhodococccus and Verrucosispora, to be also important. Thus, results from both culture-dependent and metagenomic approaches provided useful indicators on the diversity and community structure of actinobacteria endophytes in F. deltoidea.ISRA JANATININGRUMDEDY DURYADI SOLIHINANJA MERYANDINIYULIN LESTARIMBI & UNS Soloarticleactinobacteriaendophytesficus deltoideametagenomicpcr-dggeBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1514-1520 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinobacteria endophytes ficus deltoidea metagenomic pcr-dgge Biology (General) QH301-705.5
spellingShingle	actinobacteria endophytes ficus deltoidea metagenomic pcr-dgge Biology (General) QH301-705.5 ISRA JANATININGRUM DEDY DURYADI SOLIHIN ANJA MERYANDINI YULIN LESTARI Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
description	Janatiningrum I, Solihin DD, Meryandini A, Lestari Y. 2018. Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches. Biodiversitas 19: 1514-1520. Actinobacteria endophytes of medicinal plants may play an essential role in producing a variety of critical bioactive compounds. However, the possible contribution of such actinobacteria to the pharmacological properties of traditional herbal remedies remains mostly unknown. For example, the diversity and attributes of actinobacteria endophytes in Ficus deltoidea, a small tree species that has long been used to treat diseases such as cancer, diabetes, and cardiovascular illnesses, have not been explored. Here, the actinobacteria endophyte community structure in F. deltoidea was investigated using both culture-dependent and metagenomics approaches. Based on morphological characteristics and 16S rRNA gene analysis, the dominant culturable actinobacteria isolates exhibited a close relationship with Streptomyces. The metagenomic technique using PCR-DGGE analysis of the 16S rRNA gene showed the presence of 11 OTUs in F. deltoidea tissue. Whereas the dominant culturable actinobacteria endophytes in F. deltoidea was Streptomyces, the metagenomic approach showed non-Streptomyces, particularly Rhodococccus and Verrucosispora, to be also important. Thus, results from both culture-dependent and metagenomic approaches provided useful indicators on the diversity and community structure of actinobacteria endophytes in F. deltoidea.
format	article
author	ISRA JANATININGRUM DEDY DURYADI SOLIHIN ANJA MERYANDINI YULIN LESTARI
author_facet	ISRA JANATININGRUM DEDY DURYADI SOLIHIN ANJA MERYANDINI YULIN LESTARI
author_sort	ISRA JANATININGRUM
title	Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
title_short	Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
title_full	Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
title_fullStr	Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
title_full_unstemmed	Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches
title_sort	comparative study on the diversity of endophytic actinobacteria communities from ficus deltoidea using metagenomic and culture-dependent approaches
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/e7077481acd94f659e8ccbb2d8f82c48
work_keys_str_mv	AT israjanatiningrum comparativestudyonthediversityofendophyticactinobacteriacommunitiesfromficusdeltoideausingmetagenomicandculturedependentapproaches AT dedyduryadisolihin comparativestudyonthediversityofendophyticactinobacteriacommunitiesfromficusdeltoideausingmetagenomicandculturedependentapproaches AT anjameryandini comparativestudyonthediversityofendophyticactinobacteriacommunitiesfromficusdeltoideausingmetagenomicandculturedependentapproaches AT yulinlestari comparativestudyonthediversityofendophyticactinobacteriacommunitiesfromficusdeltoideausingmetagenomicandculturedependentapproaches
_version_	1718426455415717888

Comparative study on the diversity of endophytic actinobacteria communities from Ficus deltoidea using metagenomic and culture-dependent approaches

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