Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment
Abstract Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational s...
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Nature Portfolio
2021
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oai:doaj.org-article:e70980c29e214d249adcf4419e59059c2021-12-02T15:36:31ZEvaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment10.1038/s41598-021-88823-22045-2322https://doaj.org/article/e70980c29e214d249adcf4419e59059c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88823-2https://doaj.org/toc/2045-2322Abstract Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and non-responders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p < 0.01) versus the healthy cohort. After anti-TNF treatment, the phylum Proteobacteria also increased in non-responders versus responders (13/27) (p < 0.005), with the class Clostridia increasing. In addition, alpha diversity increased in responders versus non-responders (p < 0.01), tending towards eubiosis. An association was found (p < 0.001) in the F.prausnitzii/E.coli ratio between responders and non-responders. The F/E ratio was the most accurate biomarker of anti-TNF response (area under the curve 0.87). Thus, anti-TNF treatment allows partial restoration of intestinal microbiota in responders and the F.prausnitzii/E.coli ratio can provide a reliable indicator of response to anti-TNF in CD.Laura Sanchis-ArteroJuan Francisco Martínez-BlanchSergio Manresa-VeraErnesto Cortés-CastellMarina Valls-GandiaMarisa IborraJose Maria Paredes-ArquiolaMaia Boscá-WattsJose Maria HuguetRafael Gil-BorrásJosefa Rodríguez-MoralesXavier Cortés-RizoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Laura Sanchis-Artero Juan Francisco Martínez-Blanch Sergio Manresa-Vera Ernesto Cortés-Castell Marina Valls-Gandia Marisa Iborra Jose Maria Paredes-Arquiola Maia Boscá-Watts Jose Maria Huguet Rafael Gil-Borrás Josefa Rodríguez-Morales Xavier Cortés-Rizo Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| description |
Abstract Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and non-responders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p < 0.01) versus the healthy cohort. After anti-TNF treatment, the phylum Proteobacteria also increased in non-responders versus responders (13/27) (p < 0.005), with the class Clostridia increasing. In addition, alpha diversity increased in responders versus non-responders (p < 0.01), tending towards eubiosis. An association was found (p < 0.001) in the F.prausnitzii/E.coli ratio between responders and non-responders. The F/E ratio was the most accurate biomarker of anti-TNF response (area under the curve 0.87). Thus, anti-TNF treatment allows partial restoration of intestinal microbiota in responders and the F.prausnitzii/E.coli ratio can provide a reliable indicator of response to anti-TNF in CD. |
| format |
article |
| author |
Laura Sanchis-Artero Juan Francisco Martínez-Blanch Sergio Manresa-Vera Ernesto Cortés-Castell Marina Valls-Gandia Marisa Iborra Jose Maria Paredes-Arquiola Maia Boscá-Watts Jose Maria Huguet Rafael Gil-Borrás Josefa Rodríguez-Morales Xavier Cortés-Rizo |
| author_facet |
Laura Sanchis-Artero Juan Francisco Martínez-Blanch Sergio Manresa-Vera Ernesto Cortés-Castell Marina Valls-Gandia Marisa Iborra Jose Maria Paredes-Arquiola Maia Boscá-Watts Jose Maria Huguet Rafael Gil-Borrás Josefa Rodríguez-Morales Xavier Cortés-Rizo |
| author_sort |
Laura Sanchis-Artero |
| title |
Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| title_short |
Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| title_full |
Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| title_fullStr |
Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| title_full_unstemmed |
Evaluation of changes in intestinal microbiota in Crohn’s disease patients after anti-TNF alpha treatment |
| title_sort |
evaluation of changes in intestinal microbiota in crohn’s disease patients after anti-tnf alpha treatment |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e70980c29e214d249adcf4419e59059c |
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