How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways

Abstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao cou...

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Autores principales:	Jin-gao Yu, Jianming Guo, Kevin Yue Zhu, Weiwei Tao, Yanyan Chen, Pei Liu, Yongqing Hua, Yuping Tang, Jin-ao Duan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e70c3671188347a5978c6aaba1951147
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spelling	oai:doaj.org-article:e70c3671188347a5978c6aaba19511472021-12-02T15:05:33ZHow impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways10.1038/s41598-017-03201-12045-2322https://doaj.org/article/e70c3671188347a5978c6aaba19511472017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03201-1https://doaj.org/toc/2045-2322Abstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao could weaken Yuanhua’s diuretic effect, if so, which chemicals and which targets may be involved. Results showed that Yuanhua exerted diuretic effect through down-regulating renal AQP 2, without electrolyte disturbances such as K+ loss which has been observed as side-effect of most diuretics. Gancao had no diuretic effect, but could impair Yuanhua’s diuretic effect through up-regulating renal AQP 2. Glycyrrhetinic acid (GRA) in Gancao could up-regulate AQP 2 and counteract the AQP 2 regulation effect of Yuanhuacine (YHC) and Ginkwanin (GKW) in Yuanhua. Network pharmacology method suggested that YHC, GKW and GRA could bind to MEK1/FGFR1 protein and influence ERK-MAPK pathway, which was verified by Western blotting. This study supports TCM theory and reminds that more attention should be paid to the safety and efficacy problems induced by improper combination between herbs. Moreover, we suggested that promising diuretics with less side effects can be developed from Chinese Medicines such as Yuanhua.Jin-gao YuJianming GuoKevin Yue ZhuWeiwei TaoYanyan ChenPei LiuYongqing HuaYuping TangJin-ao DuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
description	Abstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao could weaken Yuanhua’s diuretic effect, if so, which chemicals and which targets may be involved. Results showed that Yuanhua exerted diuretic effect through down-regulating renal AQP 2, without electrolyte disturbances such as K+ loss which has been observed as side-effect of most diuretics. Gancao had no diuretic effect, but could impair Yuanhua’s diuretic effect through up-regulating renal AQP 2. Glycyrrhetinic acid (GRA) in Gancao could up-regulate AQP 2 and counteract the AQP 2 regulation effect of Yuanhuacine (YHC) and Ginkwanin (GKW) in Yuanhua. Network pharmacology method suggested that YHC, GKW and GRA could bind to MEK1/FGFR1 protein and influence ERK-MAPK pathway, which was verified by Western blotting. This study supports TCM theory and reminds that more attention should be paid to the safety and efficacy problems induced by improper combination between herbs. Moreover, we suggested that promising diuretics with less side effects can be developed from Chinese Medicines such as Yuanhua.
format	article
author	Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan
author_facet	Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan
author_sort	Jin-gao Yu
title	How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
title_short	How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
title_full	How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
title_fullStr	How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
title_full_unstemmed	How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
title_sort	how impaired efficacy happened between gancao and yuanhua: compounds, targets and pathways
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e70c3671188347a5978c6aaba1951147
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_version_	1718388800801996800

How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways

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