Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.

Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of the huge improvements in surgery and molecular therapy, the outcome of neoplasia remains poor. MicroRNAs are small molecules involved in several cellular processes, and their expression is altered in the vast maj...

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Autores principales:	Michela Visani, Dario de Biase, Gianluca Marucci, Cristian Taccioli, Agostino Baruzzi, Annalisa Pession, PERNO Study Group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e712a95e4dda47d4a12f0135312e0750
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spelling	oai:doaj.org-article:e712a95e4dda47d4a12f0135312e07502021-11-18T07:59:38ZDefinition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.1932-620310.1371/journal.pone.0055314https://doaj.org/article/e712a95e4dda47d4a12f0135312e07502013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383149/?tool=EBIhttps://doaj.org/toc/1932-6203Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of the huge improvements in surgery and molecular therapy, the outcome of neoplasia remains poor. MicroRNAs are small molecules involved in several cellular processes, and their expression is altered in the vast majority of tumors. Several studies reported the expression of different miRNAs in glioblastoma, but one of the most critical point in understanding glioblastoma miRNAs profile is the comparison of these studies. In this paper, we focused our attention on the non-neoplastic references used for determining miRNAs expression. The aim of this study was to investigate if using three different non-neoplastic brain references (normal adjacent the tumor, commercial total RNA, and epileptic specimens) could provide discrepant results. The analysis of 19 miRNAs was performed using Real-Time PCR, starting from the set of samples described above and the expression values compared. Moreover, the three different normal RNAs were used to determine the miRNAs profile in 30 glioblastomas. The data showed that different non-neoplastic controls could lead to different results and emphasize the importance of comparing miRNAs profiles obtained using the same experimental condition.Michela VisaniDario de BiaseGianluca MarucciCristian TaccioliAgostino BaruzziAnnalisa PessionPERNO Study GroupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e55314 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michela Visani Dario de Biase Gianluca Marucci Cristian Taccioli Agostino Baruzzi Annalisa Pession PERNO Study Group Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
description	Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of the huge improvements in surgery and molecular therapy, the outcome of neoplasia remains poor. MicroRNAs are small molecules involved in several cellular processes, and their expression is altered in the vast majority of tumors. Several studies reported the expression of different miRNAs in glioblastoma, but one of the most critical point in understanding glioblastoma miRNAs profile is the comparison of these studies. In this paper, we focused our attention on the non-neoplastic references used for determining miRNAs expression. The aim of this study was to investigate if using three different non-neoplastic brain references (normal adjacent the tumor, commercial total RNA, and epileptic specimens) could provide discrepant results. The analysis of 19 miRNAs was performed using Real-Time PCR, starting from the set of samples described above and the expression values compared. Moreover, the three different normal RNAs were used to determine the miRNAs profile in 30 glioblastomas. The data showed that different non-neoplastic controls could lead to different results and emphasize the importance of comparing miRNAs profiles obtained using the same experimental condition.
format	article
author	Michela Visani Dario de Biase Gianluca Marucci Cristian Taccioli Agostino Baruzzi Annalisa Pession PERNO Study Group
author_facet	Michela Visani Dario de Biase Gianluca Marucci Cristian Taccioli Agostino Baruzzi Annalisa Pession PERNO Study Group
author_sort	Michela Visani
title	Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
title_short	Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
title_full	Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
title_fullStr	Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
title_full_unstemmed	Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
title_sort	definition of mirnas expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e712a95e4dda47d4a12f0135312e0750
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Definition of miRNAs expression profile in glioblastoma samples: the relevance of non-neoplastic brain reference.

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