Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

AIM: To investigate whether upregulation of apoptosis-stimulating p53 protein 2 (ASPP2) expression could alleviate the development of proliferative vitreoretinopathy (PVR) in a rat model. METHODS: ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells, followed with measurement...

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Autores principales:	Yan-Kun Yue, Xiao-Li Chen, Shan Liu, Wu Liu
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	proliferative vitreoretinopathy apoptosis-stimulating p53 protein 2 epithelial–mesenchymal transition autophagy arpe-19 Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/e717ce4708df4aa795ead34b77a7227c
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spelling	oai:doaj.org-article:e717ce4708df4aa795ead34b77a7227c2021-11-26T04:02:12ZUpregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model2222-39592227-489810.18240/ijo.2021.12.02https://doaj.org/article/e717ce4708df4aa795ead34b77a7227c2021-12-01T00:00:00Zhttp://ies.ijo.cn/en_publish/2021/12/20211202.pdfhttps://doaj.org/toc/2222-3959https://doaj.org/toc/2227-4898AIM: To investigate whether upregulation of apoptosis-stimulating p53 protein 2 (ASPP2) expression could alleviate the development of proliferative vitreoretinopathy (PVR) in a rat model. METHODS: ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells, followed with measurements of cell cytotoxicity by cell counting kit-8 assay. ASPP2 upregulation was confirmed by Western blotting and immunocytochemistry. Then ARPE-19 cells pretreated with ASPP2-lentivirus were intravitreally injected to Brown Norway rats to induce PVR models. PVR development and retinal function were evaluated by retinal photography and electroretinography, respectively. Finally, epithelial-mesenchymal transition as well as autophagy were investigated in rats' retinas via Western blotting. RESULTS: Protein expression of ASPP2 was significantly upregulated by ASPP2-lentivirus transfection in ARPE-19 cells. The development and progression of PVR were impeded significantly in rats with intravitreal injection of ARPE-19 cells pretreated with ASPP2-lentivirus. Accordingly, retinal functions were less affected and PVR grades were much lower in rats with ASPP2-lentivirus compared to scrambled-lentivirus treatment. Moreover, epithelial-mesenchymal transition and autophagy markers were decreased in the retinas of rats treated with ASPP2-lentivirus. CONCLUSION: ASPP2-lentivirus transfected to ARPE-19 cells mitigates the progression of PVR in rat models, which might be partly through reduced autophagy and attenuated epithelial-mesenchymal transition. ASPP2 might stand as a new approach for PVR treatment in the future.Yan-Kun YueXiao-Li ChenShan LiuWu LiuPress of International Journal of Ophthalmology (IJO PRESS)articleproliferative vitreoretinopathyapoptosis-stimulating p53 protein 2epithelial–mesenchymal transitionautophagyarpe-19OphthalmologyRE1-994ENInternational Journal of Ophthalmology, Vol 14, Iss 12, Pp 1813-1819 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proliferative vitreoretinopathy apoptosis-stimulating p53 protein 2 epithelial–mesenchymal transition autophagy arpe-19 Ophthalmology RE1-994
spellingShingle	proliferative vitreoretinopathy apoptosis-stimulating p53 protein 2 epithelial–mesenchymal transition autophagy arpe-19 Ophthalmology RE1-994 Yan-Kun Yue Xiao-Li Chen Shan Liu Wu Liu Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
description	AIM: To investigate whether upregulation of apoptosis-stimulating p53 protein 2 (ASPP2) expression could alleviate the development of proliferative vitreoretinopathy (PVR) in a rat model. METHODS: ASPP2-lentivirus or scrambled-lentivirus were transfected into ARPE-19 cells, followed with measurements of cell cytotoxicity by cell counting kit-8 assay. ASPP2 upregulation was confirmed by Western blotting and immunocytochemistry. Then ARPE-19 cells pretreated with ASPP2-lentivirus were intravitreally injected to Brown Norway rats to induce PVR models. PVR development and retinal function were evaluated by retinal photography and electroretinography, respectively. Finally, epithelial-mesenchymal transition as well as autophagy were investigated in rats' retinas via Western blotting. RESULTS: Protein expression of ASPP2 was significantly upregulated by ASPP2-lentivirus transfection in ARPE-19 cells. The development and progression of PVR were impeded significantly in rats with intravitreal injection of ARPE-19 cells pretreated with ASPP2-lentivirus. Accordingly, retinal functions were less affected and PVR grades were much lower in rats with ASPP2-lentivirus compared to scrambled-lentivirus treatment. Moreover, epithelial-mesenchymal transition and autophagy markers were decreased in the retinas of rats treated with ASPP2-lentivirus. CONCLUSION: ASPP2-lentivirus transfected to ARPE-19 cells mitigates the progression of PVR in rat models, which might be partly through reduced autophagy and attenuated epithelial-mesenchymal transition. ASPP2 might stand as a new approach for PVR treatment in the future.
format	article
author	Yan-Kun Yue Xiao-Li Chen Shan Liu Wu Liu
author_facet	Yan-Kun Yue Xiao-Li Chen Shan Liu Wu Liu
author_sort	Yan-Kun Yue
title	Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
title_short	Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
title_full	Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
title_fullStr	Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
title_full_unstemmed	Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
title_sort	upregulation of aspp2 expression alleviates the development of proliferative vitreoretinopathy in a rat model
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/e717ce4708df4aa795ead34b77a7227c
work_keys_str_mv	AT yankunyue upregulationofaspp2expressionalleviatesthedevelopmentofproliferativevitreoretinopathyinaratmodel AT xiaolichen upregulationofaspp2expressionalleviatesthedevelopmentofproliferativevitreoretinopathyinaratmodel AT shanliu upregulationofaspp2expressionalleviatesthedevelopmentofproliferativevitreoretinopathyinaratmodel AT wuliu upregulationofaspp2expressionalleviatesthedevelopmentofproliferativevitreoretinopathyinaratmodel
_version_	1718409936860348416

Upregulation of ASPP2 expression alleviates the development of proliferative vitreoretinopathy in a rat model

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