A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency
Qingyuan Meng,1–3 Chunsheng Tao,1,4 Zhiye Qiu,1 Toshihiro Akaike,3 Fuzhai Cui,1 Xiumei Wang11School of Materials Science and Engineering, Tsinghua University, Beijing, People’s Republic of China; 2State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2015
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e71a3de33cdb4dc6827d7542ebb33308 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e71a3de33cdb4dc6827d7542ebb33308 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e71a3de33cdb4dc6827d7542ebb333082021-12-02T07:48:11ZA hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency1178-2013https://doaj.org/article/e71a3de33cdb4dc6827d7542ebb333082015-03-01T00:00:00Zhttp://www.dovepress.com/a-hybrid-substratum-for-primary-hepatocyte-culture-that-enhances-hepat-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Qingyuan Meng,1–3 Chunsheng Tao,1,4 Zhiye Qiu,1 Toshihiro Akaike,3 Fuzhai Cui,1 Xiumei Wang11School of Materials Science and Engineering, Tsinghua University, Beijing, People’s Republic of China; 2State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People’s Republic of China; 3Biomaterials Center for Regenerative Medical Engineering, Ibaraki, Japan; 4The 401 Hospital of Chinese People’s Liberation Army, Qingdao, People’s Republic of ChinaAbstract: Cell culture systems have proven to be crucial for the in vitro maintenance of primary hepatocytes and the preservation of hepatic functional expression at a high level. A poly-(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) matrix can recognize cells and promote liver function in a spheroid structure because of a specific galactose–asialoglycoprotein receptor interaction. Meanwhile, a fusion protein, E-cadherin-Fc, when incubated with various cells, has shown an enhancing effect on cellular viability and metabolism. Therefore, a hybrid substratum was developed for biomedical applications by using both of these materials to combine their advantages for primary hepatocyte cultures. The isolated cells showed a monolayer aggregate morphology on the coimmobilized surface and displayed higher functional expression than cells on traditional matrices. Furthermore, the hybrid system, in which the highest levels of cell adhesion and hepatocellular metabolism were achieved with the addition of 1% fetal bovine serum, showed a lower serum dependency than the collagen/gelatin-coated surface. Accordingly, this substrate may attenuate the negative effects of serum and further contribute to establishing a defined culture system for primary hepatocytes.Keywords: mouse primary hepatocytes, E-cadherin-Fc, PVLA, serum dependency, hybrid systemMeng QYTao CSQiu ZYAkaike TCui FZWang XMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2313-2323 (2015) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine (General) R5-920 |
| spellingShingle |
Medicine (General) R5-920 Meng QY Tao CS Qiu ZY Akaike T Cui FZ Wang XM A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| description |
Qingyuan Meng,1–3 Chunsheng Tao,1,4 Zhiye Qiu,1 Toshihiro Akaike,3 Fuzhai Cui,1 Xiumei Wang11School of Materials Science and Engineering, Tsinghua University, Beijing, People’s Republic of China; 2State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People’s Republic of China; 3Biomaterials Center for Regenerative Medical Engineering, Ibaraki, Japan; 4The 401 Hospital of Chinese People’s Liberation Army, Qingdao, People’s Republic of ChinaAbstract: Cell culture systems have proven to be crucial for the in vitro maintenance of primary hepatocytes and the preservation of hepatic functional expression at a high level. A poly-(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) matrix can recognize cells and promote liver function in a spheroid structure because of a specific galactose–asialoglycoprotein receptor interaction. Meanwhile, a fusion protein, E-cadherin-Fc, when incubated with various cells, has shown an enhancing effect on cellular viability and metabolism. Therefore, a hybrid substratum was developed for biomedical applications by using both of these materials to combine their advantages for primary hepatocyte cultures. The isolated cells showed a monolayer aggregate morphology on the coimmobilized surface and displayed higher functional expression than cells on traditional matrices. Furthermore, the hybrid system, in which the highest levels of cell adhesion and hepatocellular metabolism were achieved with the addition of 1% fetal bovine serum, showed a lower serum dependency than the collagen/gelatin-coated surface. Accordingly, this substrate may attenuate the negative effects of serum and further contribute to establishing a defined culture system for primary hepatocytes.Keywords: mouse primary hepatocytes, E-cadherin-Fc, PVLA, serum dependency, hybrid system |
| format |
article |
| author |
Meng QY Tao CS Qiu ZY Akaike T Cui FZ Wang XM |
| author_facet |
Meng QY Tao CS Qiu ZY Akaike T Cui FZ Wang XM |
| author_sort |
Meng QY |
| title |
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| title_short |
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| title_full |
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| title_fullStr |
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| title_full_unstemmed |
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| title_sort |
hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency |
| publisher |
Dove Medical Press |
| publishDate |
2015 |
| url |
https://doaj.org/article/e71a3de33cdb4dc6827d7542ebb33308 |
| work_keys_str_mv |
AT mengqy ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT taocs ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT qiuzy ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT akaiket ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT cuifz ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT wangxm ahybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT mengqy hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT taocs hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT qiuzy hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT akaiket hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT cuifz hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency AT wangxm hybridsubstratumforprimaryhepatocyteculturethatenhanceshepaticfunctionalitywithnbsplowserumdependency |
| _version_ |
1718399193181061120 |