Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells

Abstract Peritoneal fibrosis (PS) determines the long-term outcome of peritoneal dialysis (PD). We previous confirmed that hydrogen sulfide (H2S) inhibited PS, but its cellular mechanism was not fully elucidated. Epithelial-mesenchymal transition (EMT) of mesothelial cells (MCs) is an important cell...

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Autores principales:	Shengnan Cheng, Ying Lu, Yuanyuan Li, Luyan Gao, Huaying Shen, Kai Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e727474c3e5d46078bf5ac39032f174b
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spelling	oai:doaj.org-article:e727474c3e5d46078bf5ac39032f174b2021-12-02T15:09:09ZHydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells10.1038/s41598-018-21807-x2045-2322https://doaj.org/article/e727474c3e5d46078bf5ac39032f174b2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21807-xhttps://doaj.org/toc/2045-2322Abstract Peritoneal fibrosis (PS) determines the long-term outcome of peritoneal dialysis (PD). We previous confirmed that hydrogen sulfide (H2S) inhibited PS, but its cellular mechanism was not fully elucidated. Epithelial-mesenchymal transition (EMT) of mesothelial cells (MCs) is an important cellular event of PS, we therefore investigated whether EMT can be affected by H2S in MCs. Rats were treated with 4.25% -glucose PD fluids plus lipopolysaccharide for 28 days to produce PS, and NaHS (56 μg/kg.d) was given simultaneously. NaHS (56 μg/kg.d) reduced the deposition of collagen in the submesothelial zone compared with the PS group. In primarily cultured rat MCs, 4.25% -glucose PD fluid induced EMT in MCs featured as loss of ZO-1 and Cytokeratin, and increase of α-SMA, plasminogen activator inhibitor 1, fibronectin and TGF-β1 proteins. PD fluid also increased IL-6 and monocyte chemotactic protein-1 mRNA expressions as well as the phosphorylation of Smad2/3 and Smad3. NaHS (50–300 μmol/L) reversed the above alterations with the optimal dose at 100 μmol/L. Thus, exogenous H2S improves PS by inhibiting EMT in MCs. The anti-EMT effect of H2S is associated with the inhibition of inflammation and TGF-β1-Smad signal pathway.Shengnan ChengYing LuYuanyuan LiLuyan GaoHuaying ShenKai SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shengnan Cheng Ying Lu Yuanyuan Li Luyan Gao Huaying Shen Kai Song Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
description	Abstract Peritoneal fibrosis (PS) determines the long-term outcome of peritoneal dialysis (PD). We previous confirmed that hydrogen sulfide (H2S) inhibited PS, but its cellular mechanism was not fully elucidated. Epithelial-mesenchymal transition (EMT) of mesothelial cells (MCs) is an important cellular event of PS, we therefore investigated whether EMT can be affected by H2S in MCs. Rats were treated with 4.25% -glucose PD fluids plus lipopolysaccharide for 28 days to produce PS, and NaHS (56 μg/kg.d) was given simultaneously. NaHS (56 μg/kg.d) reduced the deposition of collagen in the submesothelial zone compared with the PS group. In primarily cultured rat MCs, 4.25% -glucose PD fluid induced EMT in MCs featured as loss of ZO-1 and Cytokeratin, and increase of α-SMA, plasminogen activator inhibitor 1, fibronectin and TGF-β1 proteins. PD fluid also increased IL-6 and monocyte chemotactic protein-1 mRNA expressions as well as the phosphorylation of Smad2/3 and Smad3. NaHS (50–300 μmol/L) reversed the above alterations with the optimal dose at 100 μmol/L. Thus, exogenous H2S improves PS by inhibiting EMT in MCs. The anti-EMT effect of H2S is associated with the inhibition of inflammation and TGF-β1-Smad signal pathway.
format	article
author	Shengnan Cheng Ying Lu Yuanyuan Li Luyan Gao Huaying Shen Kai Song
author_facet	Shengnan Cheng Ying Lu Yuanyuan Li Luyan Gao Huaying Shen Kai Song
author_sort	Shengnan Cheng
title	Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
title_short	Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
title_full	Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
title_fullStr	Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
title_full_unstemmed	Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
title_sort	hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e727474c3e5d46078bf5ac39032f174b
work_keys_str_mv	AT shengnancheng hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells AT yinglu hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells AT yuanyuanli hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells AT luyangao hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells AT huayingshen hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells AT kaisong hydrogensulfideinhibitsepithelialmesenchymaltransitioninperitonealmesothelialcells
_version_	1718387910016761856

Hydrogen sulfide inhibits epithelial-mesenchymal transition in peritoneal mesothelial cells

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