Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb

Blue mold pathogen, isolated from infected Allium cepa L., was identified as a Penicillium species through morphological and molecular characterisation. Internal Transcribed spacer (ITS) region of ribosomal DNA (rDNA) was utilised for DNA sequencing. Basic Local Alignment Search Tool (BLAST) analysi...

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Autores principales:	Tehzeeb Zubairi, Khajista Jabeen, Sana Khalid, Sumera Iqbal
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Penicillium specie Microscopic identification Biocontrol Pathogenicity test Phytochemical screening Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e72b6f95c72e48fabe8faba8e0e45ee8
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spelling	oai:doaj.org-article:e72b6f95c72e48fabe8faba8e0e45ee82021-11-20T04:56:24ZIsolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb1319-562X10.1016/j.sjbs.2021.07.050https://doaj.org/article/e72b6f95c72e48fabe8faba8e0e45ee82021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006331https://doaj.org/toc/1319-562XBlue mold pathogen, isolated from infected Allium cepa L., was identified as a Penicillium species through morphological and molecular characterisation. Internal Transcribed spacer (ITS) region of ribosomal DNA (rDNA) was utilised for DNA sequencing. Basic Local Alignment Search Tool (BLAST) analysis has found the maximum similarity index of the fungus to be 82.39% with the Uncultured Penicillium clone (Accession: MF535522). So, the isolated Penicillium specie is the first reported specie of the genus that infects onion. A phylogenetic tree was constructed to establish a relationship of the isolated fungus with the most relevant species reported on GenBank. Extracts of Pennisetum flaccidum Griseb. were evaluated against the isolated fungus as a potential biocontrol agent. Among the five tested methanol concentrations (0.5%, 1%, 1.5%, 2% and 2.5%) of each plant part (root, inflorescence and foliage), 0.5% root extract showed maximum growth retardation, i.e. 89%. For bioassay-guided fractionation, the root extract was partitioned in n-hexane, chloroform, n-butanol and ethyl acetate. Ethyl acetate (1%) was proved to be the most potent one. Phytochemical screening has confirmed the occurrence of terpenoids, tannins, saponins and alkaloids. The applied molecular approach has deduced that the Penicillium specie collected from Pakistan might be novel. This study can be concluded that P. flaccidum contains potent phytochemicals which might be used as antifungal agent against Penicillium species.Tehzeeb ZubairiKhajista JabeenSana KhalidSumera IqbalElsevierarticlePenicillium specieMicroscopic identificationBiocontrolPathogenicity testPhytochemical screeningBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6774-6781 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Penicillium specie Microscopic identification Biocontrol Pathogenicity test Phytochemical screening Biology (General) QH301-705.5
spellingShingle	Penicillium specie Microscopic identification Biocontrol Pathogenicity test Phytochemical screening Biology (General) QH301-705.5 Tehzeeb Zubairi Khajista Jabeen Sana Khalid Sumera Iqbal Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
description	Blue mold pathogen, isolated from infected Allium cepa L., was identified as a Penicillium species through morphological and molecular characterisation. Internal Transcribed spacer (ITS) region of ribosomal DNA (rDNA) was utilised for DNA sequencing. Basic Local Alignment Search Tool (BLAST) analysis has found the maximum similarity index of the fungus to be 82.39% with the Uncultured Penicillium clone (Accession: MF535522). So, the isolated Penicillium specie is the first reported specie of the genus that infects onion. A phylogenetic tree was constructed to establish a relationship of the isolated fungus with the most relevant species reported on GenBank. Extracts of Pennisetum flaccidum Griseb. were evaluated against the isolated fungus as a potential biocontrol agent. Among the five tested methanol concentrations (0.5%, 1%, 1.5%, 2% and 2.5%) of each plant part (root, inflorescence and foliage), 0.5% root extract showed maximum growth retardation, i.e. 89%. For bioassay-guided fractionation, the root extract was partitioned in n-hexane, chloroform, n-butanol and ethyl acetate. Ethyl acetate (1%) was proved to be the most potent one. Phytochemical screening has confirmed the occurrence of terpenoids, tannins, saponins and alkaloids. The applied molecular approach has deduced that the Penicillium specie collected from Pakistan might be novel. This study can be concluded that P. flaccidum contains potent phytochemicals which might be used as antifungal agent against Penicillium species.
format	article
author	Tehzeeb Zubairi Khajista Jabeen Sana Khalid Sumera Iqbal
author_facet	Tehzeeb Zubairi Khajista Jabeen Sana Khalid Sumera Iqbal
author_sort	Tehzeeb Zubairi
title	Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
title_short	Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
title_full	Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
title_fullStr	Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
title_full_unstemmed	Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb
title_sort	isolation and molecular characterization of causal agent of blue mold on allium cepa l. and its control by pennisetum flaccidum griseb
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e72b6f95c72e48fabe8faba8e0e45ee8
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Isolation and molecular characterization of causal agent of blue mold on Allium cepa L. and its control by Pennisetum flaccidum Griseb

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