Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.

In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the spec...

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Autores principales:	S Helene Richter, Joseph P Garner, Benjamin Zipser, Lars Lewejohann, Norbert Sachser, Chadi Touma, Britta Schindler, Sabine Chourbaji, Christiane Brandwein, Peter Gass, Niek van Stipdonk, Johanneke van der Harst, Berry Spruijt, Vootele Võikar, David P Wolfer, Hanno Würbel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e73271639bb14cb492e575ded8eb6b68
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spelling	oai:doaj.org-article:e73271639bb14cb492e575ded8eb6b682021-11-18T06:59:23ZEffect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.1932-620310.1371/journal.pone.0016461https://doaj.org/article/e73271639bb14cb492e575ded8eb6b682011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21305027/?tool=EBIhttps://doaj.org/toc/1932-6203In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies.S Helene RichterJoseph P GarnerBenjamin ZipserLars LewejohannNorbert SachserChadi ToumaBritta SchindlerSabine ChourbajiChristiane BrandweinPeter GassNiek van StipdonkJohanneke van der HarstBerry SpruijtVootele VõikarDavid P WolferHanno WürbelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16461 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S Helene Richter Joseph P Garner Benjamin Zipser Lars Lewejohann Norbert Sachser Chadi Touma Britta Schindler Sabine Chourbaji Christiane Brandwein Peter Gass Niek van Stipdonk Johanneke van der Harst Berry Spruijt Vootele Võikar David P Wolfer Hanno Würbel Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
description	In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies.
format	article
author	S Helene Richter Joseph P Garner Benjamin Zipser Lars Lewejohann Norbert Sachser Chadi Touma Britta Schindler Sabine Chourbaji Christiane Brandwein Peter Gass Niek van Stipdonk Johanneke van der Harst Berry Spruijt Vootele Võikar David P Wolfer Hanno Würbel
author_facet	S Helene Richter Joseph P Garner Benjamin Zipser Lars Lewejohann Norbert Sachser Chadi Touma Britta Schindler Sabine Chourbaji Christiane Brandwein Peter Gass Niek van Stipdonk Johanneke van der Harst Berry Spruijt Vootele Võikar David P Wolfer Hanno Würbel
author_sort	S Helene Richter
title	Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
title_short	Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
title_full	Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
title_fullStr	Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
title_full_unstemmed	Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
title_sort	effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e73271639bb14cb492e575ded8eb6b68
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Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.

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