AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure

Abstract In our present paper, the influence of a pyramidal structure on physicochemical properties of a protein in buffer solution has been studied. The pyramidal structure employed herein was similar to those produced industrially for anechoic chambers. Pyramidal structures are also used as elemen...

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Autores principales:	Yuri D. Ivanov, Tatyana O. Pleshakova, Ivan D. Shumov, Andrey F. Kozlov, Irina A. Ivanova, Anastasia A. Valueva, Maria O. Ershova, Vadim Yu. Tatur, Igor N. Stepanov, Victor V. Repnikov, Vadim S. Ziborov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e73a4a039c6647c5b58f2441b3020ff4
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spelling	oai:doaj.org-article:e73a4a039c6647c5b58f2441b3020ff42021-12-02T17:01:43ZAFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure10.1038/s41598-021-89377-z2045-2322https://doaj.org/article/e73a4a039c6647c5b58f2441b3020ff42021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89377-zhttps://doaj.org/toc/2045-2322Abstract In our present paper, the influence of a pyramidal structure on physicochemical properties of a protein in buffer solution has been studied. The pyramidal structure employed herein was similar to those produced industrially for anechoic chambers. Pyramidal structures are also used as elements of biosensors. Herein, horseradish peroxidase (HRP) enzyme was used as a model protein. HRP macromolecules were adsorbed from their solution onto an atomically smooth mica substrate, and then visualized by atomic force microscopy (AFM). In parallel, the enzymatic activity of HRP was estimated by conventional spectrophotometry. Additionally, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) has been employed in order to find out whether or not the protein secondary structure changes after the incubation of its solution either near the apex of a pyramid or in the center of its base. Using AFM, we have demonstrated that the incubation of the protein solution either in the vicinity of the pyramid’s apex or in the center of its base influences the physicochemical properties of the protein macromolecules. Namely, the incubation of the HRP solution in the vicinity of the top of the pyramidal structure has been shown to lead to an increase in the efficiency of the HRP adsorption onto mica. Moreover, after the incubation of the HRP solution either near the top of the pyramid or in the center of its base, the HRP macromolecules adsorb onto the mica surface predominantly in monomeric form. At that, the enzymatic activity of HRP does not change. The results of our present study are useful to be taken into account in the development of novel biosensor devices (including those for the diagnosis of cancer in humans), in which pyramidal structures are employed as sensor, noise suppression or construction elements.Yuri D. IvanovTatyana O. PleshakovaIvan D. ShumovAndrey F. KozlovIrina A. IvanovaAnastasia A. ValuevaMaria O. ErshovaVadim Yu. TaturIgor N. StepanovVictor V. RepnikovVadim S. ZiborovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuri D. Ivanov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Maria O. Ershova Vadim Yu. Tatur Igor N. Stepanov Victor V. Repnikov Vadim S. Ziborov AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
description	Abstract In our present paper, the influence of a pyramidal structure on physicochemical properties of a protein in buffer solution has been studied. The pyramidal structure employed herein was similar to those produced industrially for anechoic chambers. Pyramidal structures are also used as elements of biosensors. Herein, horseradish peroxidase (HRP) enzyme was used as a model protein. HRP macromolecules were adsorbed from their solution onto an atomically smooth mica substrate, and then visualized by atomic force microscopy (AFM). In parallel, the enzymatic activity of HRP was estimated by conventional spectrophotometry. Additionally, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) has been employed in order to find out whether or not the protein secondary structure changes after the incubation of its solution either near the apex of a pyramid or in the center of its base. Using AFM, we have demonstrated that the incubation of the protein solution either in the vicinity of the pyramid’s apex or in the center of its base influences the physicochemical properties of the protein macromolecules. Namely, the incubation of the HRP solution in the vicinity of the top of the pyramidal structure has been shown to lead to an increase in the efficiency of the HRP adsorption onto mica. Moreover, after the incubation of the HRP solution either near the top of the pyramid or in the center of its base, the HRP macromolecules adsorb onto the mica surface predominantly in monomeric form. At that, the enzymatic activity of HRP does not change. The results of our present study are useful to be taken into account in the development of novel biosensor devices (including those for the diagnosis of cancer in humans), in which pyramidal structures are employed as sensor, noise suppression or construction elements.
format	article
author	Yuri D. Ivanov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Maria O. Ershova Vadim Yu. Tatur Igor N. Stepanov Victor V. Repnikov Vadim S. Ziborov
author_facet	Yuri D. Ivanov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Maria O. Ershova Vadim Yu. Tatur Igor N. Stepanov Victor V. Repnikov Vadim S. Ziborov
author_sort	Yuri D. Ivanov
title	AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
title_short	AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
title_full	AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
title_fullStr	AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
title_full_unstemmed	AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
title_sort	afm study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e73a4a039c6647c5b58f2441b3020ff4
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AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure

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