The mannose receptor mediates dengue virus infection of macrophages.

Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show for the first time that the MØ mannose receptor (MR) binds to all four serotypes of DV and specifically...

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Autores principales: Joanna L Miller, Barend J M de Wet, Luisa Martinez-Pomares, Catherine M Radcliffe, Raymond A Dwek, Pauline M Rudd, Siamon Gordon
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Publicado: Public Library of Science (PLoS) 2008
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Acceso en línea:https://doaj.org/article/e746d3bd429b4310849a6e8828607251
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spelling oai:doaj.org-article:e746d3bd429b4310849a6e88286072512021-11-25T05:46:45ZThe mannose receptor mediates dengue virus infection of macrophages.1553-73661553-737410.1371/journal.ppat.0040017https://doaj.org/article/e746d3bd429b4310849a6e88286072512008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18266465/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show for the first time that the MØ mannose receptor (MR) binds to all four serotypes of DV and specifically to the envelope glycoprotein. Glycan analysis, ELISA, and blot overlay assays demonstrate that MR binds via its carbohydrate recognition domains to mosquito and human cell-produced DV antigen. This binding is abrogated by deglycosylation of the DV envelope glycoprotein. Surface expression of recombinant MR on NIH3T3 cells confers DV binding. Furthermore, DV infection of primary human MØ can be blocked by anti-MR antibodies. MR is a prototypic marker of alternatively activated MØ, and pre-treatment of human monocytes or MØ with type 2 cytokines (IL-4 or IL-13) enhances their susceptibility to productive DV infection. Our findings indicate a new functional role for the MR in DV infection.Joanna L MillerBarend J M de WetLuisa Martinez-PomaresCatherine M RadcliffeRaymond A DwekPauline M RuddSiamon GordonPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 2, p e17 (2008)
institution DOAJ
collection DOAJ
language EN
topic Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
spellingShingle Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
Joanna L Miller
Barend J M de Wet
Luisa Martinez-Pomares
Catherine M Radcliffe
Raymond A Dwek
Pauline M Rudd
Siamon Gordon
The mannose receptor mediates dengue virus infection of macrophages.
description Macrophages (MØ) and mononuclear phagocytes are major targets of infection by dengue virus (DV), a mosquito-borne flavivirus that can cause haemorrhagic fever in humans. To our knowledge, we show for the first time that the MØ mannose receptor (MR) binds to all four serotypes of DV and specifically to the envelope glycoprotein. Glycan analysis, ELISA, and blot overlay assays demonstrate that MR binds via its carbohydrate recognition domains to mosquito and human cell-produced DV antigen. This binding is abrogated by deglycosylation of the DV envelope glycoprotein. Surface expression of recombinant MR on NIH3T3 cells confers DV binding. Furthermore, DV infection of primary human MØ can be blocked by anti-MR antibodies. MR is a prototypic marker of alternatively activated MØ, and pre-treatment of human monocytes or MØ with type 2 cytokines (IL-4 or IL-13) enhances their susceptibility to productive DV infection. Our findings indicate a new functional role for the MR in DV infection.
format article
author Joanna L Miller
Barend J M de Wet
Luisa Martinez-Pomares
Catherine M Radcliffe
Raymond A Dwek
Pauline M Rudd
Siamon Gordon
author_facet Joanna L Miller
Barend J M de Wet
Luisa Martinez-Pomares
Catherine M Radcliffe
Raymond A Dwek
Pauline M Rudd
Siamon Gordon
author_sort Joanna L Miller
title The mannose receptor mediates dengue virus infection of macrophages.
title_short The mannose receptor mediates dengue virus infection of macrophages.
title_full The mannose receptor mediates dengue virus infection of macrophages.
title_fullStr The mannose receptor mediates dengue virus infection of macrophages.
title_full_unstemmed The mannose receptor mediates dengue virus infection of macrophages.
title_sort mannose receptor mediates dengue virus infection of macrophages.
publisher Public Library of Science (PLoS)
publishDate 2008
url https://doaj.org/article/e746d3bd429b4310849a6e8828607251
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