A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine

ABSTRACT Lawsonia intracellularis causes porcine proliferative enteropathy. This is an enteric disease characterized by thickening of the wall of the ileum that leads to decreased growth of animals and diarrhea. In this study, we investigated the host response to L. intracellularis infection by perf...

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Autores principales:	Fernando L. Leite, Juan E. Abrahante, Erika Vasquez, Fabio Vannucci, Connie J. Gebhart, Nathan Winkelman, Adam Mueller, Jerry Torrison, Zachary Rambo, Richard E. Isaacson
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Lawsonia intracellularis cell proliferation host response intestinal inflammation transcriptome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e74cd85830e64a768038cb0e5c3afbc0
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spelling	oai:doaj.org-article:e74cd85830e64a768038cb0e5c3afbc02021-11-15T15:55:13ZA Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine10.1128/mBio.01605-182150-7511https://doaj.org/article/e74cd85830e64a768038cb0e5c3afbc02019-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01605-18https://doaj.org/toc/2150-7511ABSTRACT Lawsonia intracellularis causes porcine proliferative enteropathy. This is an enteric disease characterized by thickening of the wall of the ileum that leads to decreased growth of animals and diarrhea. In this study, we investigated the host response to L. intracellularis infection by performing transcriptomic and pathway analysis of intestinal tissue samples from groups of infected and noninfected animals at 14, 21, and 28 days postchallenge. At the peak of infection, when animals developed the most severe lesions, infected animals had higher levels of several gene transcripts involved in cellular proliferation and inflammation, including matrix metalloproteinase-7 (MMP7), transglutaminase-2 (TGM2), and oncostatin M (OSM). Histomorphology also revealed general features of intestinal inflammation. This study identified important pathways associated with the host response in developing and resolving lesions due to L. intracellularis infection. IMPORTANCE Lawsonia intracellularis is among the most important enteric pathogens of swine, and it can also infect other mammalian species. Much is still unknown regarding its pathogenesis and the host response, especially at the site of infection. In this study, we uncovered several novel genes and pathways associated with infection. Differentially expressed transcripts, in addition to histological changes in infected tissue, revealed striking similarities between L. intracellularis infection and cellular proliferation mechanisms described in some cancers and inflammatory diseases of the gastrointestinal tract. This research sheds important light into the pathogenesis of L. intracellularis and the host response associated with the lesions caused by infection.Fernando L. LeiteJuan E. AbrahanteErika VasquezFabio VannucciConnie J. GebhartNathan WinkelmanAdam MuellerJerry TorrisonZachary RamboRichard E. IsaacsonAmerican Society for MicrobiologyarticleLawsonia intracellulariscell proliferationhost responseintestinal inflammationtranscriptomeMicrobiologyQR1-502ENmBio, Vol 10, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lawsonia intracellularis cell proliferation host response intestinal inflammation transcriptome Microbiology QR1-502
spellingShingle	Lawsonia intracellularis cell proliferation host response intestinal inflammation transcriptome Microbiology QR1-502 Fernando L. Leite Juan E. Abrahante Erika Vasquez Fabio Vannucci Connie J. Gebhart Nathan Winkelman Adam Mueller Jerry Torrison Zachary Rambo Richard E. Isaacson A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
description	ABSTRACT Lawsonia intracellularis causes porcine proliferative enteropathy. This is an enteric disease characterized by thickening of the wall of the ileum that leads to decreased growth of animals and diarrhea. In this study, we investigated the host response to L. intracellularis infection by performing transcriptomic and pathway analysis of intestinal tissue samples from groups of infected and noninfected animals at 14, 21, and 28 days postchallenge. At the peak of infection, when animals developed the most severe lesions, infected animals had higher levels of several gene transcripts involved in cellular proliferation and inflammation, including matrix metalloproteinase-7 (MMP7), transglutaminase-2 (TGM2), and oncostatin M (OSM). Histomorphology also revealed general features of intestinal inflammation. This study identified important pathways associated with the host response in developing and resolving lesions due to L. intracellularis infection. IMPORTANCE Lawsonia intracellularis is among the most important enteric pathogens of swine, and it can also infect other mammalian species. Much is still unknown regarding its pathogenesis and the host response, especially at the site of infection. In this study, we uncovered several novel genes and pathways associated with infection. Differentially expressed transcripts, in addition to histological changes in infected tissue, revealed striking similarities between L. intracellularis infection and cellular proliferation mechanisms described in some cancers and inflammatory diseases of the gastrointestinal tract. This research sheds important light into the pathogenesis of L. intracellularis and the host response associated with the lesions caused by infection.
format	article
author	Fernando L. Leite Juan E. Abrahante Erika Vasquez Fabio Vannucci Connie J. Gebhart Nathan Winkelman Adam Mueller Jerry Torrison Zachary Rambo Richard E. Isaacson
author_facet	Fernando L. Leite Juan E. Abrahante Erika Vasquez Fabio Vannucci Connie J. Gebhart Nathan Winkelman Adam Mueller Jerry Torrison Zachary Rambo Richard E. Isaacson
author_sort	Fernando L. Leite
title	A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
title_short	A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
title_full	A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
title_fullStr	A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
title_full_unstemmed	A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine
title_sort	cell proliferation and inflammatory signature is induced by <named-content content-type="genus-species">lawsonia intracellularis</named-content> infection in swine
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e74cd85830e64a768038cb0e5c3afbc0
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A Cell Proliferation and Inflammatory Signature Is Induced by <named-content content-type="genus-species">Lawsonia intracellularis</named-content> Infection in Swine

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