Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate

Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Me...

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Autores principales:	Shim KH, Hulme J, Maeng EH, Kim MK, An SSA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e750c554aa2349e69787aed117ce0623
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spelling	oai:doaj.org-article:e750c554aa2349e69787aed117ce06232021-12-02T07:15:35ZAnalysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate1178-2013https://doaj.org/article/e750c554aa2349e69787aed117ce06232014-12-01T00:00:00Zhttp://www.dovepress.com/analysis-of-sio2-nanoparticles-binding-proteins-in-rat-blood-and-brain-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes. Keywords: silica, nanoparticles, protein corona, plasma, brain homogenate, nanotoxicityShim KHHulme JMaeng EHKim MKAn SSADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Supplement 2, Pp 207-215 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shim KH Hulme J Maeng EH Kim MK An SSA Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
description	Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes. Keywords: silica, nanoparticles, protein corona, plasma, brain homogenate, nanotoxicity
format	article
author	Shim KH Hulme J Maeng EH Kim MK An SSA
author_facet	Shim KH Hulme J Maeng EH Kim MK An SSA
author_sort	Shim KH
title	Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
title_short	Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
title_full	Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
title_fullStr	Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
title_full_unstemmed	Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
title_sort	analysis of sio2 nanoparticles binding proteins in rat blood and brain homogenate
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e750c554aa2349e69787aed117ce0623
work_keys_str_mv	AT shimkh analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT hulmej analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT maengeh analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT kimmk analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT anssa analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate
_version_	1718399551388254208

Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate

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