The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase

Abstract In contrast to the widely accepted consensus of the existence of a single RNA polymerase in bacteria, several actinomycetes have been recently shown to possess two forms of RNA polymerases due the to co-existence of two rpoB paralogs in their genome. However, the biological significance of...

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Autores principales: Valeria D’Argenio, Mauro Petrillo, Daniela Pasanisi, Caterina Pagliarulo, Roberta Colicchio, Adelfia Talà, Maria Stella de Biase, Mario Zanfardino, Emanuela Scolamiero, Chiara Pagliuca, Antonio Gaballo, Annunziata Gaetana Cicatiello, Piergiuseppe Cantiello, Irene Postiglione, Barbara Naso, Angelo Boccia, Miriana Durante, Luca Cozzuto, Paola Salvatore, Giovanni Paolella, Francesco Salvatore, Pietro Alifano
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Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/e757f8c8ad234a29bc890b46372dd135
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spelling oai:doaj.org-article:e757f8c8ad234a29bc890b46372dd1352021-12-02T12:32:08ZThe complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase10.1038/s41598-016-0025-02045-2322https://doaj.org/article/e757f8c8ad234a29bc890b46372dd1352016-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-016-0025-0https://doaj.org/toc/2045-2322Abstract In contrast to the widely accepted consensus of the existence of a single RNA polymerase in bacteria, several actinomycetes have been recently shown to possess two forms of RNA polymerases due the to co-existence of two rpoB paralogs in their genome. However, the biological significance of the rpoB duplication is obscure. In this study we have determined the genome sequence of the lipoglycopeptide antibiotic A40926 producer Nonomuraea gerenzanensis ATCC 39727, an actinomycete with a large genome and two rpoB genes, i.e. rpoB(S) (the wild-type gene) and rpoB(R) (the mutant-type gene). We next analyzed the transcriptional and metabolite profiles in the wild-type gene and in two derivative strains over-expressing either rpoB(R) or a mutated form of this gene to explore the physiological role and biotechnological potential of the “mutant-type” RNA polymerase. We show that rpoB(R) controls antibiotic production and a wide range of metabolic adaptive behaviors in response to environmental pH. This may give interesting perspectives also with regard to biotechnological applications.Valeria D’ArgenioMauro PetrilloDaniela PasanisiCaterina PagliaruloRoberta ColicchioAdelfia TalàMaria Stella de BiaseMario ZanfardinoEmanuela ScolamieroChiara PagliucaAntonio GaballoAnnunziata Gaetana CicatielloPiergiuseppe CantielloIrene PostiglioneBarbara NasoAngelo BocciaMiriana DuranteLuca CozzutoPaola SalvatoreGiovanni PaolellaFrancesco SalvatorePietro AlifanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 6, Iss 1, Pp 1-13 (2016)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Valeria D’Argenio
Mauro Petrillo
Daniela Pasanisi
Caterina Pagliarulo
Roberta Colicchio
Adelfia Talà
Maria Stella de Biase
Mario Zanfardino
Emanuela Scolamiero
Chiara Pagliuca
Antonio Gaballo
Annunziata Gaetana Cicatiello
Piergiuseppe Cantiello
Irene Postiglione
Barbara Naso
Angelo Boccia
Miriana Durante
Luca Cozzuto
Paola Salvatore
Giovanni Paolella
Francesco Salvatore
Pietro Alifano
The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
description Abstract In contrast to the widely accepted consensus of the existence of a single RNA polymerase in bacteria, several actinomycetes have been recently shown to possess two forms of RNA polymerases due the to co-existence of two rpoB paralogs in their genome. However, the biological significance of the rpoB duplication is obscure. In this study we have determined the genome sequence of the lipoglycopeptide antibiotic A40926 producer Nonomuraea gerenzanensis ATCC 39727, an actinomycete with a large genome and two rpoB genes, i.e. rpoB(S) (the wild-type gene) and rpoB(R) (the mutant-type gene). We next analyzed the transcriptional and metabolite profiles in the wild-type gene and in two derivative strains over-expressing either rpoB(R) or a mutated form of this gene to explore the physiological role and biotechnological potential of the “mutant-type” RNA polymerase. We show that rpoB(R) controls antibiotic production and a wide range of metabolic adaptive behaviors in response to environmental pH. This may give interesting perspectives also with regard to biotechnological applications.
format article
author Valeria D’Argenio
Mauro Petrillo
Daniela Pasanisi
Caterina Pagliarulo
Roberta Colicchio
Adelfia Talà
Maria Stella de Biase
Mario Zanfardino
Emanuela Scolamiero
Chiara Pagliuca
Antonio Gaballo
Annunziata Gaetana Cicatiello
Piergiuseppe Cantiello
Irene Postiglione
Barbara Naso
Angelo Boccia
Miriana Durante
Luca Cozzuto
Paola Salvatore
Giovanni Paolella
Francesco Salvatore
Pietro Alifano
author_facet Valeria D’Argenio
Mauro Petrillo
Daniela Pasanisi
Caterina Pagliarulo
Roberta Colicchio
Adelfia Talà
Maria Stella de Biase
Mario Zanfardino
Emanuela Scolamiero
Chiara Pagliuca
Antonio Gaballo
Annunziata Gaetana Cicatiello
Piergiuseppe Cantiello
Irene Postiglione
Barbara Naso
Angelo Boccia
Miriana Durante
Luca Cozzuto
Paola Salvatore
Giovanni Paolella
Francesco Salvatore
Pietro Alifano
author_sort Valeria D’Argenio
title The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
title_short The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
title_full The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
title_fullStr The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
title_full_unstemmed The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated “magic” RNA polymerase
title_sort complete 12 mb genome and transcriptome of nonomuraea gerenzanensis with new insights into its duplicated “magic” rna polymerase
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/e757f8c8ad234a29bc890b46372dd135
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