Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption

Abstract Our group recently identified 16 genes whose peripheral blood expression levels are differentially regulated in acute ischemic stroke. The purpose of this study was to determine whether the early expression levels of any of these 16 genes are predictive for post-stroke blood brain barrier (...

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Autores principales:	Grant C. O’Connell, Madison B. Treadway, Ashley B. Petrone, Connie S. Tennant, Noelle Lucke-Wold, Paul D. Chantler, Taura L. Barr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e76b9946d4db45bcba4dbd9774e5d292
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spelling	oai:doaj.org-article:e76b9946d4db45bcba4dbd9774e5d2922021-12-02T15:06:21ZPeripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption10.1038/s41598-017-01178-52045-2322https://doaj.org/article/e76b9946d4db45bcba4dbd9774e5d2922017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01178-5https://doaj.org/toc/2045-2322Abstract Our group recently identified 16 genes whose peripheral blood expression levels are differentially regulated in acute ischemic stroke. The purpose of this study was to determine whether the early expression levels of any of these 16 genes are predictive for post-stroke blood brain barrier (BBB) disruption. Transcriptional expression levels of candidate genes were measured in peripheral blood sampled from ischemic stroke patients at emergency department admission, and BBB permeability was assessed at 24 hour follow up via perfusion-weighted imaging. Early heightened expression levels of AKAP7, a gene encoding a protein kinase A-binding scaffolding molecule, were significantly associated with BBB disruption 24 hours post-hospital admission. We then determined that AKAP7 is predominantly expressed by lymphocytes in peripheral blood, and strongly co-expressed with ITGA3, a gene encoding the adhesion molecule integrin alpha 3. Subsequent in vitro experiments revealed that heightened expression of AKAP7 and ITGA3 in primary human lymphocytes is associated with a highly adherent phenotype. Collectively, our results suggest that AKAP7 expression levels may have clinical utility as a prognostic biomarker for post-stroke BBB complications, and are likely elevated early in patients who later develop post-stroke BBB disruption due to the presence of an invasive lymphocyte population in the peripheral blood.Grant C. O’ConnellMadison B. TreadwayAshley B. PetroneConnie S. TennantNoelle Lucke-WoldPaul D. ChantlerTaura L. BarrNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Grant C. O’Connell Madison B. Treadway Ashley B. Petrone Connie S. Tennant Noelle Lucke-Wold Paul D. Chantler Taura L. Barr Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
description	Abstract Our group recently identified 16 genes whose peripheral blood expression levels are differentially regulated in acute ischemic stroke. The purpose of this study was to determine whether the early expression levels of any of these 16 genes are predictive for post-stroke blood brain barrier (BBB) disruption. Transcriptional expression levels of candidate genes were measured in peripheral blood sampled from ischemic stroke patients at emergency department admission, and BBB permeability was assessed at 24 hour follow up via perfusion-weighted imaging. Early heightened expression levels of AKAP7, a gene encoding a protein kinase A-binding scaffolding molecule, were significantly associated with BBB disruption 24 hours post-hospital admission. We then determined that AKAP7 is predominantly expressed by lymphocytes in peripheral blood, and strongly co-expressed with ITGA3, a gene encoding the adhesion molecule integrin alpha 3. Subsequent in vitro experiments revealed that heightened expression of AKAP7 and ITGA3 in primary human lymphocytes is associated with a highly adherent phenotype. Collectively, our results suggest that AKAP7 expression levels may have clinical utility as a prognostic biomarker for post-stroke BBB complications, and are likely elevated early in patients who later develop post-stroke BBB disruption due to the presence of an invasive lymphocyte population in the peripheral blood.
format	article
author	Grant C. O’Connell Madison B. Treadway Ashley B. Petrone Connie S. Tennant Noelle Lucke-Wold Paul D. Chantler Taura L. Barr
author_facet	Grant C. O’Connell Madison B. Treadway Ashley B. Petrone Connie S. Tennant Noelle Lucke-Wold Paul D. Chantler Taura L. Barr
author_sort	Grant C. O’Connell
title	Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
title_short	Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
title_full	Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
title_fullStr	Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
title_full_unstemmed	Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
title_sort	peripheral blood akap7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e76b9946d4db45bcba4dbd9774e5d292
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Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption

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