Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

Background: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and in...

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Autores principales:	Nathalia M. Correa Valencia, Nicolás F. Ramírez, Michael Bülte, Jorge A. Fernández Silva
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2017
Materias:	culture medium elisa johne's disease map molecular diagnosis Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/e77473c0a313485299c7c3450def3a52
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spelling	oai:doaj.org-article:e77473c0a313485299c7c3450def3a522021-11-26T19:19:23ZFecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd2256-295810.17533/udea.rccp.v30n2a02https://doaj.org/article/e77473c0a313485299c7c3450def3a522017-04-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/327053https://doaj.org/toc/2256-2958Background: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest. Objective: to report the diagnosis and detection of MAP using several diagnostic tests in a herd of the Northern region of Antioquia, Colombia. Methods: serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecal samples were cultured by duplicate using Herrold´s egg yolk medium (HEYM), and analyzed by an endpoint IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit. Results: eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Seven of the 27 fecal samples were found to be positive by end-point IS900-specific nested PCR. Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals. Conclusion: the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions.Nathalia M. Correa ValenciaNicolás F. RamírezMichael BülteJorge A. Fernández SilvaUniversidad de Antioquiaarticleculture mediumelisajohne's diseasemapmolecular diagnosisAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 30, Iss 2, Pp 101-115 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	culture medium elisa johne's disease map molecular diagnosis Animal culture SF1-1100
spellingShingle	culture medium elisa johne's disease map molecular diagnosis Animal culture SF1-1100 Nathalia M. Correa Valencia Nicolás F. Ramírez Michael Bülte Jorge A. Fernández Silva Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
description	Background: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest. Objective: to report the diagnosis and detection of MAP using several diagnostic tests in a herd of the Northern region of Antioquia, Colombia. Methods: serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecal samples were cultured by duplicate using Herrold´s egg yolk medium (HEYM), and analyzed by an endpoint IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit. Results: eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Seven of the 27 fecal samples were found to be positive by end-point IS900-specific nested PCR. Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals. Conclusion: the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions.
format	article
author	Nathalia M. Correa Valencia Nicolás F. Ramírez Michael Bülte Jorge A. Fernández Silva
author_facet	Nathalia M. Correa Valencia Nicolás F. Ramírez Michael Bülte Jorge A. Fernández Silva
author_sort	Nathalia M. Correa Valencia
title	Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
title_short	Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
title_full	Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
title_fullStr	Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
title_full_unstemmed	Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
title_sort	fecal culture and two fecal-pcr methods for the diagnosis of mycobacterium avium subsp. paratuberculosis in a seropositive herd
publisher	Universidad de Antioquia
publishDate	2017
url	https://doaj.org/article/e77473c0a313485299c7c3450def3a52
work_keys_str_mv	AT nathaliamcorreavalencia fecalcultureandtwofecalpcrmethodsforthediagnosisofmycobacteriumaviumsubspparatuberculosisinaseropositiveherd AT nicolasframirez fecalcultureandtwofecalpcrmethodsforthediagnosisofmycobacteriumaviumsubspparatuberculosisinaseropositiveherd AT michaelbulte fecalcultureandtwofecalpcrmethodsforthediagnosisofmycobacteriumaviumsubspparatuberculosisinaseropositiveherd AT jorgeafernandezsilva fecalcultureandtwofecalpcrmethodsforthediagnosisofmycobacteriumaviumsubspparatuberculosisinaseropositiveherd
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Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

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