Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease

Abstract Asthma—chronic obstructive pulmonary disease (COPD) overlap, termed as ACO, is a complex heterogeneous disease characterised by persistent airflow limitation, which manifests features of both asthma and COPD. These patients have a worse prognosis, in terms of more frequent and severe exacer...

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Autores principales:	Nilanjana Ghosh, Priyanka Choudhury, Mamata Joshi, Parthasarathi Bhattacharyya, Sushmita Roychowdhury, Rintu Banerjee, Koel Chaudhury
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e776e262ecd84ba19dd9fb7bfd250b5a
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spelling	oai:doaj.org-article:e776e262ecd84ba19dd9fb7bfd250b5a2021-12-02T17:08:43ZGlobal metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease10.1038/s41598-021-96128-72045-2322https://doaj.org/article/e776e262ecd84ba19dd9fb7bfd250b5a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96128-7https://doaj.org/toc/2045-2322Abstract Asthma—chronic obstructive pulmonary disease (COPD) overlap, termed as ACO, is a complex heterogeneous disease characterised by persistent airflow limitation, which manifests features of both asthma and COPD. These patients have a worse prognosis, in terms of more frequent and severe exacerbations, more frequent symptoms, worse quality of life, increased comorbidities and a faster lung function decline. In absence of clear diagnostic or therapeutic guidelines, ACO presents as a challenge to clinicians. The present study aims to investigate whether ACO patients have a distinct exhaled breath condensate (EBC) metabolic profile in comparison to asthma and COPD. A total of 132 age and BMI matched male smokers were recruited in the exploratory phase which consisted of (i) controls = 33 (ii) asthma = 34 (iii) COPD = 30 and (iv) ACO = 35. Using nuclear magnetic resonance (NMR) metabolomics, 8 metabolites (fatty acid, propionate, isopropanol, lactate, acetone, valine, methanol and formate) were identified to be significantly dysregulated in ACO subjects when compared to both, asthma and COPD. The expression of these dysregulated metabolites were further validated in a fresh patient cohort consisting of (i) asthma = 32 (ii) COPD = 32 and (iii) ACO = 40, which exhibited a similar expression pattern. Multivariate receiver operating characteristic (ROC) curves generated using these metabolites provided a robust ACO classification model. The findings were also integrated with previously identified serum metabolites and inflammatory markers to develop a robust predictive model for differentiation of ACO. Our findings suggest that NMR metabolomics of EBC holds potential as a platform to identify robust, non-invasive biomarkers for differentiating ACO from asthma and COPD.Nilanjana GhoshPriyanka ChoudhuryMamata JoshiParthasarathi BhattacharyyaSushmita RoychowdhuryRintu BanerjeeKoel ChaudhuryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nilanjana Ghosh Priyanka Choudhury Mamata Joshi Parthasarathi Bhattacharyya Sushmita Roychowdhury Rintu Banerjee Koel Chaudhury Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
description	Abstract Asthma—chronic obstructive pulmonary disease (COPD) overlap, termed as ACO, is a complex heterogeneous disease characterised by persistent airflow limitation, which manifests features of both asthma and COPD. These patients have a worse prognosis, in terms of more frequent and severe exacerbations, more frequent symptoms, worse quality of life, increased comorbidities and a faster lung function decline. In absence of clear diagnostic or therapeutic guidelines, ACO presents as a challenge to clinicians. The present study aims to investigate whether ACO patients have a distinct exhaled breath condensate (EBC) metabolic profile in comparison to asthma and COPD. A total of 132 age and BMI matched male smokers were recruited in the exploratory phase which consisted of (i) controls = 33 (ii) asthma = 34 (iii) COPD = 30 and (iv) ACO = 35. Using nuclear magnetic resonance (NMR) metabolomics, 8 metabolites (fatty acid, propionate, isopropanol, lactate, acetone, valine, methanol and formate) were identified to be significantly dysregulated in ACO subjects when compared to both, asthma and COPD. The expression of these dysregulated metabolites were further validated in a fresh patient cohort consisting of (i) asthma = 32 (ii) COPD = 32 and (iii) ACO = 40, which exhibited a similar expression pattern. Multivariate receiver operating characteristic (ROC) curves generated using these metabolites provided a robust ACO classification model. The findings were also integrated with previously identified serum metabolites and inflammatory markers to develop a robust predictive model for differentiation of ACO. Our findings suggest that NMR metabolomics of EBC holds potential as a platform to identify robust, non-invasive biomarkers for differentiating ACO from asthma and COPD.
format	article
author	Nilanjana Ghosh Priyanka Choudhury Mamata Joshi Parthasarathi Bhattacharyya Sushmita Roychowdhury Rintu Banerjee Koel Chaudhury
author_facet	Nilanjana Ghosh Priyanka Choudhury Mamata Joshi Parthasarathi Bhattacharyya Sushmita Roychowdhury Rintu Banerjee Koel Chaudhury
author_sort	Nilanjana Ghosh
title	Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
title_short	Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
title_full	Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
title_fullStr	Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
title_full_unstemmed	Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease
title_sort	global metabolome profiling of exhaled breath condensates in male smokers with asthma copd overlap and prediction of the disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e776e262ecd84ba19dd9fb7bfd250b5a
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Global metabolome profiling of exhaled breath condensates in male smokers with asthma COPD overlap and prediction of the disease

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