A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection

Abstract Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity, as well as rapid or real-time operation. Furthermore, microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation. Herein, we present microfabr...

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Autores principales:	Naixin Song, Pengfei Xie, Wen Shen, Hanju Oh, Yejia Zhang, Flavia Vitale, Mehdi Javanmard, Mark G. Allen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Technology T Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/e77907fa947f4086aba9e1301378c41a
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spelling	oai:doaj.org-article:e77907fa947f4086aba9e1301378c41a2021-11-28T12:07:59ZA microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection10.1038/s41378-021-00297-42055-7434https://doaj.org/article/e77907fa947f4086aba9e1301378c41a2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41378-021-00297-4https://doaj.org/toc/2055-7434Abstract Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity, as well as rapid or real-time operation. Furthermore, microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation. Herein, we present microfabricated needle-shaped microwell impedance sensors for rapid-sample-to-answer, label-free detection of cytokines, and other biomarkers. The microneedle form factor allows sensors to be utilized in transcutaneous or transvascular sensing applications. In vitro, experimental characterization confirmed sensor specificity and sensitivity to multiple proteins of interest. Mechanical characterization demonstrated sufficient microneedle robustness for transcutaneous insertion, as well as preserved sensor function postinsertion. We further utilized these sensors to carry out real-time in vivo quantification of human interleukin 8 (hIL8) concentration levels in the blood of transgenic mice that endogenously express hIL8. To assess sensor functionality, hIL8 concentration levels in serum samples from the same mice were quantified by ELISA. Excellent agreement between real-time in vivo sensor readings in blood and subsequent ELISA serum assays was observed over multiple transgenic mice expressing hIL8 concentrations from 62 pg/mL to 539 ng/mL.Naixin SongPengfei XieWen ShenHanju OhYejia ZhangFlavia VitaleMehdi JavanmardMark G. AllenNature Publishing GrouparticleTechnologyTEngineering (General). Civil engineering (General)TA1-2040ENMicrosystems & Nanoengineering, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Technology T Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	Technology T Engineering (General). Civil engineering (General) TA1-2040 Naixin Song Pengfei Xie Wen Shen Hanju Oh Yejia Zhang Flavia Vitale Mehdi Javanmard Mark G. Allen A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
description	Abstract Impedance-based protein detection sensors for point-of-care diagnostics require quantitative specificity, as well as rapid or real-time operation. Furthermore, microfabrication of these sensors can lead to the formation of factors suitable for in vivo operation. Herein, we present microfabricated needle-shaped microwell impedance sensors for rapid-sample-to-answer, label-free detection of cytokines, and other biomarkers. The microneedle form factor allows sensors to be utilized in transcutaneous or transvascular sensing applications. In vitro, experimental characterization confirmed sensor specificity and sensitivity to multiple proteins of interest. Mechanical characterization demonstrated sufficient microneedle robustness for transcutaneous insertion, as well as preserved sensor function postinsertion. We further utilized these sensors to carry out real-time in vivo quantification of human interleukin 8 (hIL8) concentration levels in the blood of transgenic mice that endogenously express hIL8. To assess sensor functionality, hIL8 concentration levels in serum samples from the same mice were quantified by ELISA. Excellent agreement between real-time in vivo sensor readings in blood and subsequent ELISA serum assays was observed over multiple transgenic mice expressing hIL8 concentrations from 62 pg/mL to 539 ng/mL.
format	article
author	Naixin Song Pengfei Xie Wen Shen Hanju Oh Yejia Zhang Flavia Vitale Mehdi Javanmard Mark G. Allen
author_facet	Naixin Song Pengfei Xie Wen Shen Hanju Oh Yejia Zhang Flavia Vitale Mehdi Javanmard Mark G. Allen
author_sort	Naixin Song
title	A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
title_short	A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
title_full	A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
title_fullStr	A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
title_full_unstemmed	A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
title_sort	microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/e77907fa947f4086aba9e1301378c41a
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A microwell-based impedance sensor on an insertable microneedle for real-time in vivo cytokine detection

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