Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery

Abstract Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-tr...

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Autores principales:	Swati Mishra, James Dee, William Moar, Jodi Dufner-Beattie, James Baum, Naymã Pinto Dias, Andrei Alyokhin, Aaron Buzza, Silvia I. Rondon, Mark Clough, Sandy Menasha, Russell Groves, Justin Clements, Ken Ostlie, Gary Felton, Tim Waters, William E. Snyder, Juan Luis Jurat-Fuentes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e780da9e18344236be16c74090991e43
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spelling	oai:doaj.org-article:e780da9e18344236be16c74090991e432021-12-02T11:45:02ZSelection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery10.1038/s41598-021-85876-12045-2322https://doaj.org/article/e780da9e18344236be16c74090991e432021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85876-1https://doaj.org/toc/2045-2322Abstract Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population of Colorado potato beetle (Leptinotarsa decemlineata Say) (Coleoptera: Chrysomelidae) with > 11,100-fold resistance to a dsRNA targeting the V-ATPase subunit A gene after nine episodes of selection using non-transgenic delivery by foliar coating. Resistance was associated with lack of target gene down-regulation in CEAS 300 larvae and cross-resistance to another dsRNA target (COPI β; Coatomer subunit beta). In contrast, CEAS 300 larvae showed very low (~ 4-fold) reduced susceptibility to the Cry3Aa insecticidal protein from Bacillus thuringiensis. Resistance to dsRNA in CEAS 300 is transmitted as an autosomal recessive trait and is polygenic. These data represent the first documented case of resistance in an insect pest with high pesticide resistance potential using dsRNA delivered through non-transgenic techniques. Information on the genetics of resistance and availability of dsRNA-resistant L. decemlineata guide the design of resistance management tools and allow research to identify resistance alleles and estimate resistance risks.Swati MishraJames DeeWilliam MoarJodi Dufner-BeattieJames BaumNaymã Pinto DiasAndrei AlyokhinAaron BuzzaSilvia I. RondonMark CloughSandy MenashaRussell GrovesJustin ClementsKen OstlieGary FeltonTim WatersWilliam E. SnyderJuan Luis Jurat-FuentesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Swati Mishra James Dee William Moar Jodi Dufner-Beattie James Baum Naymã Pinto Dias Andrei Alyokhin Aaron Buzza Silvia I. Rondon Mark Clough Sandy Menasha Russell Groves Justin Clements Ken Ostlie Gary Felton Tim Waters William E. Snyder Juan Luis Jurat-Fuentes Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
description	Abstract Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population of Colorado potato beetle (Leptinotarsa decemlineata Say) (Coleoptera: Chrysomelidae) with > 11,100-fold resistance to a dsRNA targeting the V-ATPase subunit A gene after nine episodes of selection using non-transgenic delivery by foliar coating. Resistance was associated with lack of target gene down-regulation in CEAS 300 larvae and cross-resistance to another dsRNA target (COPI β; Coatomer subunit beta). In contrast, CEAS 300 larvae showed very low (~ 4-fold) reduced susceptibility to the Cry3Aa insecticidal protein from Bacillus thuringiensis. Resistance to dsRNA in CEAS 300 is transmitted as an autosomal recessive trait and is polygenic. These data represent the first documented case of resistance in an insect pest with high pesticide resistance potential using dsRNA delivered through non-transgenic techniques. Information on the genetics of resistance and availability of dsRNA-resistant L. decemlineata guide the design of resistance management tools and allow research to identify resistance alleles and estimate resistance risks.
format	article
author	Swati Mishra James Dee William Moar Jodi Dufner-Beattie James Baum Naymã Pinto Dias Andrei Alyokhin Aaron Buzza Silvia I. Rondon Mark Clough Sandy Menasha Russell Groves Justin Clements Ken Ostlie Gary Felton Tim Waters William E. Snyder Juan Luis Jurat-Fuentes
author_facet	Swati Mishra James Dee William Moar Jodi Dufner-Beattie James Baum Naymã Pinto Dias Andrei Alyokhin Aaron Buzza Silvia I. Rondon Mark Clough Sandy Menasha Russell Groves Justin Clements Ken Ostlie Gary Felton Tim Waters William E. Snyder Juan Luis Jurat-Fuentes
author_sort	Swati Mishra
title	Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
title_short	Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
title_full	Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
title_fullStr	Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
title_full_unstemmed	Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery
title_sort	selection for high levels of resistance to double-stranded rna (dsrna) in colorado potato beetle (leptinotarsa decemlineata say) using non-transgenic foliar delivery
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e780da9e18344236be16c74090991e43
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Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery

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