Visualization and analysis of 3D microscopic images.
In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we disc...
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2012
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oai:doaj.org-article:e7845a128cda4655abed2dd3f69742982021-11-18T05:51:16ZVisualization and analysis of 3D microscopic images.1553-734X1553-735810.1371/journal.pcbi.1002519https://doaj.org/article/e7845a128cda4655abed2dd3f69742982012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22719236/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain.Fuhui LongJianlong ZhouHanchuan PengPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 8, Iss 6, p e1002519 (2012) |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Fuhui Long Jianlong Zhou Hanchuan Peng Visualization and analysis of 3D microscopic images. |
description |
In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain. |
format |
article |
author |
Fuhui Long Jianlong Zhou Hanchuan Peng |
author_facet |
Fuhui Long Jianlong Zhou Hanchuan Peng |
author_sort |
Fuhui Long |
title |
Visualization and analysis of 3D microscopic images. |
title_short |
Visualization and analysis of 3D microscopic images. |
title_full |
Visualization and analysis of 3D microscopic images. |
title_fullStr |
Visualization and analysis of 3D microscopic images. |
title_full_unstemmed |
Visualization and analysis of 3D microscopic images. |
title_sort |
visualization and analysis of 3d microscopic images. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doaj.org/article/e7845a128cda4655abed2dd3f6974298 |
work_keys_str_mv |
AT fuhuilong visualizationandanalysisof3dmicroscopicimages AT jianlongzhou visualizationandanalysisof3dmicroscopicimages AT hanchuanpeng visualizationandanalysisof3dmicroscopicimages |
_version_ |
1718424762420559872 |