Visualization and analysis of 3D microscopic images.

In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we disc...

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Autores principales: Fuhui Long, Jianlong Zhou, Hanchuan Peng
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Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/e7845a128cda4655abed2dd3f6974298
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spelling oai:doaj.org-article:e7845a128cda4655abed2dd3f69742982021-11-18T05:51:16ZVisualization and analysis of 3D microscopic images.1553-734X1553-735810.1371/journal.pcbi.1002519https://doaj.org/article/e7845a128cda4655abed2dd3f69742982012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22719236/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain.Fuhui LongJianlong ZhouHanchuan PengPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 8, Iss 6, p e1002519 (2012)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Fuhui Long
Jianlong Zhou
Hanchuan Peng
Visualization and analysis of 3D microscopic images.
description In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain.
format article
author Fuhui Long
Jianlong Zhou
Hanchuan Peng
author_facet Fuhui Long
Jianlong Zhou
Hanchuan Peng
author_sort Fuhui Long
title Visualization and analysis of 3D microscopic images.
title_short Visualization and analysis of 3D microscopic images.
title_full Visualization and analysis of 3D microscopic images.
title_fullStr Visualization and analysis of 3D microscopic images.
title_full_unstemmed Visualization and analysis of 3D microscopic images.
title_sort visualization and analysis of 3d microscopic images.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/e7845a128cda4655abed2dd3f6974298
work_keys_str_mv AT fuhuilong visualizationandanalysisof3dmicroscopicimages
AT jianlongzhou visualizationandanalysisof3dmicroscopicimages
AT hanchuanpeng visualizationandanalysisof3dmicroscopicimages
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