Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.

Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acqui...

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Autores principales:	Luca Fieramonti, Andrea Bassi, Efrem Alessandro Foglia, Anna Pistocchi, Cosimo D'Andrea, Gianluca Valentini, Rinaldo Cubeddu, Sandro De Silvestri, Giulio Cerullo, Franco Cotelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e78beb9fa5314881bb445257195efe61
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id	oai:doaj.org-article:e78beb9fa5314881bb445257195efe61
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spelling	oai:doaj.org-article:e78beb9fa5314881bb445257195efe612021-11-18T08:08:13ZTime-gated optical projection tomography allows visualization of adult zebrafish internal structures.1932-620310.1371/journal.pone.0050744https://doaj.org/article/e78beb9fa5314881bb445257195efe612012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23185643/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acquisition, allows the reduction of scattering effects in tomographic imaging. This technique, namely Time-Gated Optical Projection Tomography (TGOPT), is used to reconstruct three dimensionally the internal structure of adult zebrafish without staining or clearing agents. This method extends the use of Optical Projection Tomography to optically diffusive samples yielding reconstructions with reduced artifacts, increased contrast and improved resolution with respect to those obtained with non-gated techniques. The paper shows that TGOPT is particularly suited for imaging the skeletal system and nervous structures of adult zebrafish.Luca FieramontiAndrea BassiEfrem Alessandro FogliaAnna PistocchiCosimo D'AndreaGianluca ValentiniRinaldo CubedduSandro De SilvestriGiulio CerulloFranco CotelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50744 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luca Fieramonti Andrea Bassi Efrem Alessandro Foglia Anna Pistocchi Cosimo D'Andrea Gianluca Valentini Rinaldo Cubeddu Sandro De Silvestri Giulio Cerullo Franco Cotelli Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
description	Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acquisition, allows the reduction of scattering effects in tomographic imaging. This technique, namely Time-Gated Optical Projection Tomography (TGOPT), is used to reconstruct three dimensionally the internal structure of adult zebrafish without staining or clearing agents. This method extends the use of Optical Projection Tomography to optically diffusive samples yielding reconstructions with reduced artifacts, increased contrast and improved resolution with respect to those obtained with non-gated techniques. The paper shows that TGOPT is particularly suited for imaging the skeletal system and nervous structures of adult zebrafish.
format	article
author	Luca Fieramonti Andrea Bassi Efrem Alessandro Foglia Anna Pistocchi Cosimo D'Andrea Gianluca Valentini Rinaldo Cubeddu Sandro De Silvestri Giulio Cerullo Franco Cotelli
author_facet	Luca Fieramonti Andrea Bassi Efrem Alessandro Foglia Anna Pistocchi Cosimo D'Andrea Gianluca Valentini Rinaldo Cubeddu Sandro De Silvestri Giulio Cerullo Franco Cotelli
author_sort	Luca Fieramonti
title	Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
title_short	Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
title_full	Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
title_fullStr	Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
title_full_unstemmed	Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
title_sort	time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e78beb9fa5314881bb445257195efe61
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Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.

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