Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate

Abstract The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conse...

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Autores principales:	Yue Li, Guorui Li, Huajun Sun, Yongsheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e79369e58d1a40178eb0cce9b1f09291
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spelling	oai:doaj.org-article:e79369e58d1a40178eb0cce9b1f092912021-12-02T16:35:56ZCharacterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate10.1038/s41598-021-86305-z2045-2322https://doaj.org/article/e79369e58d1a40178eb0cce9b1f092912021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86305-zhttps://doaj.org/toc/2045-2322Abstract The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). This enzyme exhibited the greatest activity and tolerance to chloroform and toluene when it was expressed in Pichia pastoris GS115 at 40 ℃ and pH 8.0. Zn and Cu ions exerted obvious inhibitory effects on the enzyme, and displayed good hydrolytic activity for long-chain natural and synthetic lipids. HPLC analysis showed that this enzyme has 1,3 regioselectivity when glycerol tripalmitate and oleic acid are used as substrates. The fatty acid composition in the reaction product was 21.3% oleic acid and 79.1% sn-2 palmitic acid.Yue LiGuorui LiHuajun SunYongsheng ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yue Li Guorui Li Huajun Sun Yongsheng Chen Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
description	Abstract The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). This enzyme exhibited the greatest activity and tolerance to chloroform and toluene when it was expressed in Pichia pastoris GS115 at 40 ℃ and pH 8.0. Zn and Cu ions exerted obvious inhibitory effects on the enzyme, and displayed good hydrolytic activity for long-chain natural and synthetic lipids. HPLC analysis showed that this enzyme has 1,3 regioselectivity when glycerol tripalmitate and oleic acid are used as substrates. The fatty acid composition in the reaction product was 21.3% oleic acid and 79.1% sn-2 palmitic acid.
format	article
author	Yue Li Guorui Li Huajun Sun Yongsheng Chen
author_facet	Yue Li Guorui Li Huajun Sun Yongsheng Chen
author_sort	Yue Li
title	Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
title_short	Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
title_full	Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
title_fullStr	Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
title_full_unstemmed	Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate
title_sort	characterization of a novel sn1,3 lipase from ricinus communis l. suitable for production of oleic acid-palmitic acid-glycerol oleate
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e79369e58d1a40178eb0cce9b1f09291
work_keys_str_mv	AT yueli characterizationofanovelsn13lipasefromricinuscommunislsuitableforproductionofoleicacidpalmiticacidglycerololeate AT guoruili characterizationofanovelsn13lipasefromricinuscommunislsuitableforproductionofoleicacidpalmiticacidglycerololeate AT huajunsun characterizationofanovelsn13lipasefromricinuscommunislsuitableforproductionofoleicacidpalmiticacidglycerololeate AT yongshengchen characterizationofanovelsn13lipasefromricinuscommunislsuitableforproductionofoleicacidpalmiticacidglycerololeate
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Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-glycerol oleate

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