Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine
Jonna Nykky, Jenni E Tuusa, Sanna Kirjavainen, Matti Vuento, Leona GilbertNanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, FinlandAbstract: Viruses have great potential as nanotools in medicine for gene transfer, targeted g...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2010
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e7945a27eed94a8dae11128ef961e213 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e7945a27eed94a8dae11128ef961e213 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e7945a27eed94a8dae11128ef961e2132021-12-02T00:39:11ZMechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine1176-91141178-2013https://doaj.org/article/e7945a27eed94a8dae11128ef961e2132010-06-01T00:00:00Zhttp://www.dovepress.com/mechanisms-of-cell-death-in-canine-parvovirus-infected-cells-provide-i-a4721https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jonna Nykky, Jenni E Tuusa, Sanna Kirjavainen, Matti Vuento, Leona GilbertNanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, FinlandAbstract: Viruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was further confirmed by activation of caspases and cellular DNA damage. However, results from annexin V-propidium iodide (PI) labeling and membrane polarization assays indicated disruption of the plasma membrane uncommon to apoptosis. These results provide evidence that secondary necrosis followed apoptosis. In addition, two human cancer cell lines were found to be infected by CPV. This necrotic event over apoptotic cell death and infection in human cells provide insightful information when developing CPV as a nanotool for cancer treatments.Keywords: canine parvovirus, apoptosis, necrosis, nanoparticle, virotherapy Jonna NykkyJenni E TuusaSanna Kirjavainenet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 417-428 (2010) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine (General) R5-920 |
| spellingShingle |
Medicine (General) R5-920 Jonna Nykky Jenni E Tuusa Sanna Kirjavainen et al Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| description |
Jonna Nykky, Jenni E Tuusa, Sanna Kirjavainen, Matti Vuento, Leona GilbertNanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, FinlandAbstract: Viruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was further confirmed by activation of caspases and cellular DNA damage. However, results from annexin V-propidium iodide (PI) labeling and membrane polarization assays indicated disruption of the plasma membrane uncommon to apoptosis. These results provide evidence that secondary necrosis followed apoptosis. In addition, two human cancer cell lines were found to be infected by CPV. This necrotic event over apoptotic cell death and infection in human cells provide insightful information when developing CPV as a nanotool for cancer treatments.Keywords: canine parvovirus, apoptosis, necrosis, nanoparticle, virotherapy |
| format |
article |
| author |
Jonna Nykky Jenni E Tuusa Sanna Kirjavainen et al |
| author_facet |
Jonna Nykky Jenni E Tuusa Sanna Kirjavainen et al |
| author_sort |
Jonna Nykky |
| title |
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| title_short |
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| title_full |
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| title_fullStr |
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| title_full_unstemmed |
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| title_sort |
mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine |
| publisher |
Dove Medical Press |
| publishDate |
2010 |
| url |
https://doaj.org/article/e7945a27eed94a8dae11128ef961e213 |
| work_keys_str_mv |
AT jonnanykky mechanismsofcelldeathincanineparvovirusinfectedcellsprovideintuitiveinsightstodevelopingnanotoolsformedicine AT jennietuusa mechanismsofcelldeathincanineparvovirusinfectedcellsprovideintuitiveinsightstodevelopingnanotoolsformedicine AT sannakirjavainen mechanismsofcelldeathincanineparvovirusinfectedcellsprovideintuitiveinsightstodevelopingnanotoolsformedicine AT etal mechanismsofcelldeathincanineparvovirusinfectedcellsprovideintuitiveinsightstodevelopingnanotoolsformedicine |
| _version_ |
1718403590527123456 |