Rheotaxis-based sperm separation using a biomimicry microfluidic device

Abstract Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry mi...

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Autores principales:	Iman Ramazani Sarbandi, Ali Lesani, Mahdi Moghimi Zand, Reza Nosrati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e79be4609d5e4232a0ba07ce09d2e903
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spelling	oai:doaj.org-article:e79be4609d5e4232a0ba07ce09d2e9032021-12-02T18:50:03ZRheotaxis-based sperm separation using a biomimicry microfluidic device10.1038/s41598-021-97602-y2045-2322https://doaj.org/article/e79be4609d5e4232a0ba07ce09d2e9032021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97602-yhttps://doaj.org/toc/2045-2322Abstract Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry microfluidic method, inspired by the anatomy of the female reproductive tract, that separates motile sperm based on their rheotaxis behavior to swim against the flow into low shear rate regions. The device includes micropocket geometries that recall the oval-shaped microstructures of the female fallopian tube to create shear protected zones for sperm separation. Clinical tests with human samples indicate that the device is capable of isolating viable and highly motile sperm based on their rheotaxis responses, resulting in a separation efficiency of 100%. The device presents an automated alternative for the current sperm selection practices in assisted reproduction.Iman Ramazani SarbandiAli LesaniMahdi Moghimi ZandReza NosratiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iman Ramazani Sarbandi Ali Lesani Mahdi Moghimi Zand Reza Nosrati Rheotaxis-based sperm separation using a biomimicry microfluidic device
description	Abstract Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry microfluidic method, inspired by the anatomy of the female reproductive tract, that separates motile sperm based on their rheotaxis behavior to swim against the flow into low shear rate regions. The device includes micropocket geometries that recall the oval-shaped microstructures of the female fallopian tube to create shear protected zones for sperm separation. Clinical tests with human samples indicate that the device is capable of isolating viable and highly motile sperm based on their rheotaxis responses, resulting in a separation efficiency of 100%. The device presents an automated alternative for the current sperm selection practices in assisted reproduction.
format	article
author	Iman Ramazani Sarbandi Ali Lesani Mahdi Moghimi Zand Reza Nosrati
author_facet	Iman Ramazani Sarbandi Ali Lesani Mahdi Moghimi Zand Reza Nosrati
author_sort	Iman Ramazani Sarbandi
title	Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_short	Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_full	Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_fullStr	Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_full_unstemmed	Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_sort	rheotaxis-based sperm separation using a biomimicry microfluidic device
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e79be4609d5e4232a0ba07ce09d2e903
work_keys_str_mv	AT imanramazanisarbandi rheotaxisbasedspermseparationusingabiomimicrymicrofluidicdevice AT alilesani rheotaxisbasedspermseparationusingabiomimicrymicrofluidicdevice AT mahdimoghimizand rheotaxisbasedspermseparationusingabiomimicrymicrofluidicdevice AT rezanosrati rheotaxisbasedspermseparationusingabiomimicrymicrofluidicdevice
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Rheotaxis-based sperm separation using a biomimicry microfluidic device

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