Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli

New Delhi metallo-β-lactamases (NDMs), including at least 28 variants, are a rapidly emerging family of β-lactamases worldwide, with a variety of infections caused by NDM-positive strains usually associated with very poor prognosis and high mortality. NDMs are the most prevalent carbapenemases in Es...

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Autores principales:	Zhiren Zhang, Hengzhao Guo, Xiaodong Li, Wenting Li, Guang Yang, Wenjun Ni, Meixiao Zhan, Ligong Lu, Zhenlin Zhang, Xiaobin Li, Zhiling Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Escherichia coli New Delhi metallo-β-lactamase plasmid conjugative mobilizable Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e7a07fb51b914ba9a9e8ed3334e4f443
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spelling	oai:doaj.org-article:e7a07fb51b914ba9a9e8ed3334e4f4432021-11-17T16:42:12ZGenetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli1664-302X10.3389/fmicb.2021.729952https://doaj.org/article/e7a07fb51b914ba9a9e8ed3334e4f4432021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.729952/fullhttps://doaj.org/toc/1664-302XNew Delhi metallo-β-lactamases (NDMs), including at least 28 variants, are a rapidly emerging family of β-lactamases worldwide, with a variety of infections caused by NDM-positive strains usually associated with very poor prognosis and high mortality. NDMs are the most prevalent carbapenemases in Escherichia coli (E. coli) worldwide, especially in China. The vast majority of blaNDM cases occur on plasmids, which play a vital role in the dissemination of blaNDM. To systematically explore the relationships between plasmids and blaNDM genes in E. coli and obtain an overall picture of the conjugative and mobilizable blaNDM-positive plasmids, we analyzed the variants of blaNDM, replicon types, phylogenetic patterns, conjugative transfer modules, host STs, and geographical distributions of 114 blaNDM-positive plasmids, which were selected from 3786 plasmids from 1346 complete whole genomes of E. coli from the GenBank database. We also established links among the characteristics of blaNDM-positive plasmids in E. coli. Eight variants of blaNDM were found among the 114 blaNDM-positive plasmids, with blaNDM–5 (74 blaNDM–5 genes in 73 plasmids), and blaNDM–1 (31 blaNDM–1 genes in 28 plasmids) being the most dominant. The variant blaNDM–5 was mainly carried by the IncX3 plasmids and IncF plasmids in E. coli, the former were mainly geographically distributed in East Asia (especially in China) and the United States, and the latter were widely distributed worldwide. IncC plasmids were observed to be the predominant carriers of blaNDM–1 genes in E. coli, which were mainly geographically distributed in the United States and China. Other blaNDM–1-carrying plasmids also included IncM2, IncN2, and IncHI1. Moreover, the overall picture of the conjugative and mobilizable blaNDM-positive plasmids in E. coli was described in our study. Our findings enhance our understanding of the genetic diversity and characteristics of blaNDM-positive plasmids in in E. coli.Zhiren ZhangHengzhao GuoXiaodong LiWenting LiGuang YangWenjun NiMeixiao ZhanMeixiao ZhanLigong LuLigong LuZhenlin ZhangXiaobin LiZhiling ZhouFrontiers Media S.A.articleEscherichia coliNew Delhi metallo-β-lactamaseplasmidconjugativemobilizableMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli New Delhi metallo-β-lactamase plasmid conjugative mobilizable Microbiology QR1-502
spellingShingle	Escherichia coli New Delhi metallo-β-lactamase plasmid conjugative mobilizable Microbiology QR1-502 Zhiren Zhang Hengzhao Guo Xiaodong Li Wenting Li Guang Yang Wenjun Ni Meixiao Zhan Meixiao Zhan Ligong Lu Ligong Lu Zhenlin Zhang Xiaobin Li Zhiling Zhou Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
description	New Delhi metallo-β-lactamases (NDMs), including at least 28 variants, are a rapidly emerging family of β-lactamases worldwide, with a variety of infections caused by NDM-positive strains usually associated with very poor prognosis and high mortality. NDMs are the most prevalent carbapenemases in Escherichia coli (E. coli) worldwide, especially in China. The vast majority of blaNDM cases occur on plasmids, which play a vital role in the dissemination of blaNDM. To systematically explore the relationships between plasmids and blaNDM genes in E. coli and obtain an overall picture of the conjugative and mobilizable blaNDM-positive plasmids, we analyzed the variants of blaNDM, replicon types, phylogenetic patterns, conjugative transfer modules, host STs, and geographical distributions of 114 blaNDM-positive plasmids, which were selected from 3786 plasmids from 1346 complete whole genomes of E. coli from the GenBank database. We also established links among the characteristics of blaNDM-positive plasmids in E. coli. Eight variants of blaNDM were found among the 114 blaNDM-positive plasmids, with blaNDM–5 (74 blaNDM–5 genes in 73 plasmids), and blaNDM–1 (31 blaNDM–1 genes in 28 plasmids) being the most dominant. The variant blaNDM–5 was mainly carried by the IncX3 plasmids and IncF plasmids in E. coli, the former were mainly geographically distributed in East Asia (especially in China) and the United States, and the latter were widely distributed worldwide. IncC plasmids were observed to be the predominant carriers of blaNDM–1 genes in E. coli, which were mainly geographically distributed in the United States and China. Other blaNDM–1-carrying plasmids also included IncM2, IncN2, and IncHI1. Moreover, the overall picture of the conjugative and mobilizable blaNDM-positive plasmids in E. coli was described in our study. Our findings enhance our understanding of the genetic diversity and characteristics of blaNDM-positive plasmids in in E. coli.
format	article
author	Zhiren Zhang Hengzhao Guo Xiaodong Li Wenting Li Guang Yang Wenjun Ni Meixiao Zhan Meixiao Zhan Ligong Lu Ligong Lu Zhenlin Zhang Xiaobin Li Zhiling Zhou
author_facet	Zhiren Zhang Hengzhao Guo Xiaodong Li Wenting Li Guang Yang Wenjun Ni Meixiao Zhan Meixiao Zhan Ligong Lu Ligong Lu Zhenlin Zhang Xiaobin Li Zhiling Zhou
author_sort	Zhiren Zhang
title	Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
title_short	Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
title_full	Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
title_fullStr	Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
title_full_unstemmed	Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli
title_sort	genetic diversity and characteristics of blandm-positive plasmids in escherichia coli
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e7a07fb51b914ba9a9e8ed3334e4f443
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Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli

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