Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions

Aim. To establish  correlations between the "acidic" and "basic" proteins in different structures of colon hyperplastic polyps, adenomas and adenocarcinomas with use of histochemical staining. Material and Methods. For the research, 20 histological cases of colon polyps were u...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: O.Yu. Varуvoda, I.S. Davydenko, O.V. Garvasyuk
Formato: article
Lenguaje:EN
UK
Publicado: Danylo Halytsky Lviv National Medical University 2018
Materias:
Acceso en línea:https://doaj.org/article/e7a4d1a365f24950b384e7431c012738
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:e7a4d1a365f24950b384e7431c012738
record_format dspace
spelling oai:doaj.org-article:e7a4d1a365f24950b384e7431c0127382021-11-10T19:50:03ZStudying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions1029-42442415-330310.25040/aml2018.03.037https://doaj.org/article/e7a4d1a365f24950b384e7431c0127382018-09-01T00:00:00Zhttps://amljournal.com/index.php/journal/article/view/94https://doaj.org/toc/1029-4244https://doaj.org/toc/2415-3303Aim. To establish  correlations between the "acidic" and "basic" proteins in different structures of colon hyperplastic polyps, adenomas and adenocarcinomas with use of histochemical staining. Material and Methods. For the research, 20 histological cases of colon polyps were used. The material was taken from patients who died from other somatic diseases. There were four study groups, each included 5 cases (n=20) of hyperplastic colon polyps, adenomas with high grade or low grade dysplasia and colon adenocarcinomas. The control group contained epithelial cells (conditional norm) of intestinal crypts outside the tumor in patients with cancer. The secondary index - the R/B coefficient was calculated, and then the results were compared between the study groups. Results and Discussion. The mean value of the R/B coefficient in the cytoplasm of the round, low grade differentiated adenocarcinoma cells amounts to 1,24±0,017. The mean value of the R/B coefficient in the cytoplasm of the polygonal adenocarcinoma cells amounts to 1,11±0,012. The mean value of the epithelial cells cytoplasm coefficient in hyperplastic colon polyps amounts to 1,09±0,011. The mean value of the epithelial cells cytoplasm coefficient in colon adenomas with low grade dysplasia amounts to 1,21±0,016. The mean value of epithelial cells cytoplasm coefficient (conditional norm) of intestinal crypts outside the tumor in patients with cancer equals 1,04±0,012. Conclusions. Low grade differentiated colon adenocarcinoma is characterized by predominance of the "acidic" proteins over the "basic" proteins in the tumor epithelial cells. We also found a difference in correlations between the "acidic" proteins and the "basic" proteins inadenomas groups with high grade or low grade dysplasia. Consequently, with the increase of the dysplasia degree up to the low-differentiated adenocarcinoma, the difference in the ratio of the R/B coefficient also increases.O.Yu. VarуvodaI.S. DavydenkoO.V. GarvasyukDanylo Halytsky Lviv National Medical Universityarticlecolon, adenocarcinoma, proteins properties, histochemical staining, polypMedicine (General)R5-920ENUKActa Medica Leopoliensia, Vol 24, Iss 3, Pp 37-41 (2018)
institution DOAJ
collection DOAJ
language EN
UK
topic colon, adenocarcinoma, proteins properties, histochemical staining, polyp
Medicine (General)
R5-920
spellingShingle colon, adenocarcinoma, proteins properties, histochemical staining, polyp
Medicine (General)
R5-920
O.Yu. Varуvoda
I.S. Davydenko
O.V. Garvasyuk
Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
description Aim. To establish  correlations between the "acidic" and "basic" proteins in different structures of colon hyperplastic polyps, adenomas and adenocarcinomas with use of histochemical staining. Material and Methods. For the research, 20 histological cases of colon polyps were used. The material was taken from patients who died from other somatic diseases. There were four study groups, each included 5 cases (n=20) of hyperplastic colon polyps, adenomas with high grade or low grade dysplasia and colon adenocarcinomas. The control group contained epithelial cells (conditional norm) of intestinal crypts outside the tumor in patients with cancer. The secondary index - the R/B coefficient was calculated, and then the results were compared between the study groups. Results and Discussion. The mean value of the R/B coefficient in the cytoplasm of the round, low grade differentiated adenocarcinoma cells amounts to 1,24±0,017. The mean value of the R/B coefficient in the cytoplasm of the polygonal adenocarcinoma cells amounts to 1,11±0,012. The mean value of the epithelial cells cytoplasm coefficient in hyperplastic colon polyps amounts to 1,09±0,011. The mean value of the epithelial cells cytoplasm coefficient in colon adenomas with low grade dysplasia amounts to 1,21±0,016. The mean value of epithelial cells cytoplasm coefficient (conditional norm) of intestinal crypts outside the tumor in patients with cancer equals 1,04±0,012. Conclusions. Low grade differentiated colon adenocarcinoma is characterized by predominance of the "acidic" proteins over the "basic" proteins in the tumor epithelial cells. We also found a difference in correlations between the "acidic" proteins and the "basic" proteins inadenomas groups with high grade or low grade dysplasia. Consequently, with the increase of the dysplasia degree up to the low-differentiated adenocarcinoma, the difference in the ratio of the R/B coefficient also increases.
format article
author O.Yu. Varуvoda
I.S. Davydenko
O.V. Garvasyuk
author_facet O.Yu. Varуvoda
I.S. Davydenko
O.V. Garvasyuk
author_sort O.Yu. Varуvoda
title Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
title_short Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
title_full Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
title_fullStr Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
title_full_unstemmed Studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
title_sort studying of oxidation modification of proteins in epithelial cells of the colon with polyposy lesions
publisher Danylo Halytsky Lviv National Medical University
publishDate 2018
url https://doaj.org/article/e7a4d1a365f24950b384e7431c012738
work_keys_str_mv AT oyuvaruvoda studyingofoxidationmodificationofproteinsinepithelialcellsofthecolonwithpolyposylesions
AT isdavydenko studyingofoxidationmodificationofproteinsinepithelialcellsofthecolonwithpolyposylesions
AT ovgarvasyuk studyingofoxidationmodificationofproteinsinepithelialcellsofthecolonwithpolyposylesions
_version_ 1718439731839107072