Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions

In patients with high-grade squamous intraepithelial lesion (HSIL) of the vulva, the presence of multiple lesions, called multifocal HSIL, is common. The aim of this exploratory study was to investigate biomarker expression profiles in multifocal HSIL. In total, 27 lesions from 12 patients with high...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nikki B. Thuijs, Willemijn A. M. Schonck, Linde L. J. Klaver, Guus Fons, Marc van Beurden, Renske D. M. Steenbergen, Maaike C. G. Bleeker
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vulvar intraepithelial neoplasia HPV biomarker methylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e7a73b064b5946cbbce6f897e4411bb4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e7a73b064b5946cbbce6f897e4411bb4
record_format	dspace
spelling	oai:doaj.org-article:e7a73b064b5946cbbce6f897e4411bb42021-11-25T17:02:02ZBiomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions10.3390/cancers132256462072-6694https://doaj.org/article/e7a73b064b5946cbbce6f897e4411bb42021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5646https://doaj.org/toc/2072-6694In patients with high-grade squamous intraepithelial lesion (HSIL) of the vulva, the presence of multiple lesions, called multifocal HSIL, is common. The aim of this exploratory study was to investigate biomarker expression profiles in multifocal HSIL. In total, 27 lesions from 12 patients with high-risk human papillomavirus (HPV)-positive multifocal HSIL were tested for HPV genotype, expression of p16<sup>INK4a</sup> and Ki-67, and DNA methylation of six genes. HPV16 was found most commonly in 21 (77.8%) HSILs. In two (16.4%) patients, HPV genotype differed between the lesions. All lesions demonstrated diffuse p16<sup>INK4a</sup> staining, of which three (11.1%) were combined with patchy staining. One patient (8.3%) demonstrated markedly different DNA methylation levels between lesions. Generally, heterogeneity in methylation profiles was observed between different patients, even when other biomarkers showed similar expression. In conclusion, this study is the first to demonstrate heterogeneity of individual lesions in patients with multifocal HSIL. The studied biomarkers have the potential to refine prognostic and predictive diagnostics. Future prospective, longitudinal studies are needed to further explore the potential of a biomarker profile for management of patients with multifocal HSIL.Nikki B. ThuijsWillemijn A. M. SchonckLinde L. J. KlaverGuus FonsMarc van BeurdenRenske D. M. SteenbergenMaaike C. G. BleekerMDPI AGarticlevulvar intraepithelial neoplasiaHPVbiomarkermethylationNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5646, p 5646 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vulvar intraepithelial neoplasia HPV biomarker methylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	vulvar intraepithelial neoplasia HPV biomarker methylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Nikki B. Thuijs Willemijn A. M. Schonck Linde L. J. Klaver Guus Fons Marc van Beurden Renske D. M. Steenbergen Maaike C. G. Bleeker Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
description	In patients with high-grade squamous intraepithelial lesion (HSIL) of the vulva, the presence of multiple lesions, called multifocal HSIL, is common. The aim of this exploratory study was to investigate biomarker expression profiles in multifocal HSIL. In total, 27 lesions from 12 patients with high-risk human papillomavirus (HPV)-positive multifocal HSIL were tested for HPV genotype, expression of p16<sup>INK4a</sup> and Ki-67, and DNA methylation of six genes. HPV16 was found most commonly in 21 (77.8%) HSILs. In two (16.4%) patients, HPV genotype differed between the lesions. All lesions demonstrated diffuse p16<sup>INK4a</sup> staining, of which three (11.1%) were combined with patchy staining. One patient (8.3%) demonstrated markedly different DNA methylation levels between lesions. Generally, heterogeneity in methylation profiles was observed between different patients, even when other biomarkers showed similar expression. In conclusion, this study is the first to demonstrate heterogeneity of individual lesions in patients with multifocal HSIL. The studied biomarkers have the potential to refine prognostic and predictive diagnostics. Future prospective, longitudinal studies are needed to further explore the potential of a biomarker profile for management of patients with multifocal HSIL.
format	article
author	Nikki B. Thuijs Willemijn A. M. Schonck Linde L. J. Klaver Guus Fons Marc van Beurden Renske D. M. Steenbergen Maaike C. G. Bleeker
author_facet	Nikki B. Thuijs Willemijn A. M. Schonck Linde L. J. Klaver Guus Fons Marc van Beurden Renske D. M. Steenbergen Maaike C. G. Bleeker
author_sort	Nikki B. Thuijs
title	Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
title_short	Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
title_full	Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
title_fullStr	Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
title_full_unstemmed	Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions
title_sort	biomarker expression in multifocal vulvar high-grade squamous intraepithelial lesions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e7a73b064b5946cbbce6f897e4411bb4
work_keys_str_mv	AT nikkibthuijs biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT willemijnamschonck biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT lindeljklaver biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT guusfons biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT marcvanbeurden biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT renskedmsteenbergen biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions AT maaikecgbleeker biomarkerexpressioninmultifocalvulvarhighgradesquamousintraepitheliallesions
_version_	1718412810732437504

Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions

Ejemplares similares