Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

Abstract Human exposure to lead mainly occurs by ingestion of contaminated food, water and soil. Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of Escherichia coli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Changye Hui, Yan Guo, Wen Zhang, Chaoxian Gao, Xueqin Yang, Yuting Chen, Limei Li, Xianqing Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7ad51536f4e4c449c90c8e01914899b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e7ad51536f4e4c449c90c8e01914899b
record_format	dspace
spelling	oai:doaj.org-article:e7ad51536f4e4c449c90c8e01914899b2021-12-02T15:07:50ZSurface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice10.1038/s41598-018-24134-32045-2322https://doaj.org/article/e7ad51536f4e4c449c90c8e01914899b2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24134-3https://doaj.org/toc/2045-2322Abstract Human exposure to lead mainly occurs by ingestion of contaminated food, water and soil. Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of Escherichia coli (E. coli), a predominant strain among intestinal microflora, using lipoprotein (Lpp)-OmpA as the anchoring protein. In vitro, the PbrR displayed cells had an enhanced ability for immobilizing toxic lead(II) ions from the external media at both acidic and neutral pH, and exhibited a higher specific adsorption for lead compared to other physiological two valence metal ions. In vivo, the persistence of recombinant E. coli in the murine intestinal tract and the integrity of surface displayed PbrR were confirmed. In addition, oral administration of surface-engineered E. coli was safe in mice, in which the concentrations of physiological metal ions in blood were not affected. More importantly, lead associated with PbrR-displayed E. coli was demonstrated to be less bioavailable in the experimental mouse model with exposure to oral lead. This is reflected by significantly lower blood and femur lead concentrations in PbrR-displayed E. coli groups compared to the control. These results open up the possibility for the removal of toxic metal ions in vivo using engineered microorganisms as adsorbents.Changye HuiYan GuoWen ZhangChaoxian GaoXueqin YangYuting ChenLimei LiXianqing HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Changye Hui Yan Guo Wen Zhang Chaoxian Gao Xueqin Yang Yuting Chen Limei Li Xianqing Huang Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
description	Abstract Human exposure to lead mainly occurs by ingestion of contaminated food, water and soil. Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of Escherichia coli (E. coli), a predominant strain among intestinal microflora, using lipoprotein (Lpp)-OmpA as the anchoring protein. In vitro, the PbrR displayed cells had an enhanced ability for immobilizing toxic lead(II) ions from the external media at both acidic and neutral pH, and exhibited a higher specific adsorption for lead compared to other physiological two valence metal ions. In vivo, the persistence of recombinant E. coli in the murine intestinal tract and the integrity of surface displayed PbrR were confirmed. In addition, oral administration of surface-engineered E. coli was safe in mice, in which the concentrations of physiological metal ions in blood were not affected. More importantly, lead associated with PbrR-displayed E. coli was demonstrated to be less bioavailable in the experimental mouse model with exposure to oral lead. This is reflected by significantly lower blood and femur lead concentrations in PbrR-displayed E. coli groups compared to the control. These results open up the possibility for the removal of toxic metal ions in vivo using engineered microorganisms as adsorbents.
format	article
author	Changye Hui Yan Guo Wen Zhang Chaoxian Gao Xueqin Yang Yuting Chen Limei Li Xianqing Huang
author_facet	Changye Hui Yan Guo Wen Zhang Chaoxian Gao Xueqin Yang Yuting Chen Limei Li Xianqing Huang
author_sort	Changye Hui
title	Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
title_short	Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
title_full	Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
title_fullStr	Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
title_full_unstemmed	Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
title_sort	surface display of pbrr on escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e7ad51536f4e4c449c90c8e01914899b
work_keys_str_mv	AT changyehui surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT yanguo surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT wenzhang surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT chaoxiangao surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT xueqinyang surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT yutingchen surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT limeili surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice AT xianqinghuang surfacedisplayofpbrronescherichiacoliandevaluationofthebioavailabilityofleadassociatedwithengineeredcellsinmice
_version_	1718388421462851584

Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

Ejemplares similares