Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)

While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Elena Trizna, Diana Baidamshina, Anna Gorshkova, Valentin Drucker, Mikhail Bogachev, Anton Tikhonov, Airat Kayumov
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
Acceso en línea:https://doaj.org/article/e7b121de72664b9faabb29cfa34ea540
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Descripción
Sumario:While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide (Longidaza<sup>®</sup>) destroys both mono- and dual-species biofilms formed by various bacteria. After 4 h of treatment with 750 units of the enzyme, the residual biofilms of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i> preserved about 50–70% of their initial mass. Biomasses of dual-species biofilms formed by <i>S. aureus</i> and the four latter species were reduced 1.5-fold after 24 h treatment, while the significant destruction of <i>S. aureus–P. aeruginosa</i> and <i>S. aureus–K. pneumoniae</i> was also observed after 4 h of treatment with Longidaza<sup>®</sup>. Furthermore, when applied in combination, Longidaza<sup>®</sup> increased the efficacy of various antimicrobials against biofilm-embedded bacteria, although with various increase-factor values depending on both the bacterial species and antimicrobials chosen. Taken together, our data indicate that Longidaza<sup>®</sup> destroys the biofilm structure, facilitating the penetration of antimicrobials through the biofilm, and in this way improving their efficacy, lowering the required dose and thus also potentially reducing the associated side effects.