Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)

While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elena Trizna, Diana Baidamshina, Anna Gorshkova, Valentin Drucker, Mikhail Bogachev, Anton Tikhonov, Airat Kayumov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacterial biofilms enzymatic destruction of the biofilm bovine hyaluronidase azoximer (Longidaza) Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/e7b121de72664b9faabb29cfa34ea540
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e7b121de72664b9faabb29cfa34ea540
record_format	dspace
spelling	oai:doaj.org-article:e7b121de72664b9faabb29cfa34ea5402021-11-25T18:40:17ZImproving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)10.3390/pharmaceutics131117401999-4923https://doaj.org/article/e7b121de72664b9faabb29cfa34ea5402021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1740https://doaj.org/toc/1999-4923While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide (Longidaza<sup>®</sup>) destroys both mono- and dual-species biofilms formed by various bacteria. After 4 h of treatment with 750 units of the enzyme, the residual biofilms of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i> preserved about 50–70% of their initial mass. Biomasses of dual-species biofilms formed by <i>S. aureus</i> and the four latter species were reduced 1.5-fold after 24 h treatment, while the significant destruction of <i>S. aureus–P. aeruginosa</i> and <i>S. aureus–K. pneumoniae</i> was also observed after 4 h of treatment with Longidaza<sup>®</sup>. Furthermore, when applied in combination, Longidaza<sup>®</sup> increased the efficacy of various antimicrobials against biofilm-embedded bacteria, although with various increase-factor values depending on both the bacterial species and antimicrobials chosen. Taken together, our data indicate that Longidaza<sup>®</sup> destroys the biofilm structure, facilitating the penetration of antimicrobials through the biofilm, and in this way improving their efficacy, lowering the required dose and thus also potentially reducing the associated side effects.Elena TriznaDiana BaidamshinaAnna GorshkovaValentin DruckerMikhail BogachevAnton TikhonovAirat KayumovMDPI AGarticlebacterial biofilmsenzymatic destruction of the biofilmbovine hyaluronidase azoximer (Longidaza)Pharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1740, p 1740 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial biofilms enzymatic destruction of the biofilm bovine hyaluronidase azoximer (Longidaza) Pharmacy and materia medica RS1-441
spellingShingle	bacterial biofilms enzymatic destruction of the biofilm bovine hyaluronidase azoximer (Longidaza) Pharmacy and materia medica RS1-441 Elena Trizna Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Anton Tikhonov Airat Kayumov Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
description	While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide (Longidaza<sup>®</sup>) destroys both mono- and dual-species biofilms formed by various bacteria. After 4 h of treatment with 750 units of the enzyme, the residual biofilms of <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i> preserved about 50–70% of their initial mass. Biomasses of dual-species biofilms formed by <i>S. aureus</i> and the four latter species were reduced 1.5-fold after 24 h treatment, while the significant destruction of <i>S. aureus–P. aeruginosa</i> and <i>S. aureus–K. pneumoniae</i> was also observed after 4 h of treatment with Longidaza<sup>®</sup>. Furthermore, when applied in combination, Longidaza<sup>®</sup> increased the efficacy of various antimicrobials against biofilm-embedded bacteria, although with various increase-factor values depending on both the bacterial species and antimicrobials chosen. Taken together, our data indicate that Longidaza<sup>®</sup> destroys the biofilm structure, facilitating the penetration of antimicrobials through the biofilm, and in this way improving their efficacy, lowering the required dose and thus also potentially reducing the associated side effects.
format	article
author	Elena Trizna Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Anton Tikhonov Airat Kayumov
author_facet	Elena Trizna Diana Baidamshina Anna Gorshkova Valentin Drucker Mikhail Bogachev Anton Tikhonov Airat Kayumov
author_sort	Elena Trizna
title	Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
title_short	Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
title_full	Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
title_fullStr	Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
title_full_unstemmed	Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)
title_sort	improving the efficacy of antimicrobials against biofilm-embedded bacteria using bovine hyaluronidase azoximer (longidaza<sup>®</sup>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e7b121de72664b9faabb29cfa34ea540
work_keys_str_mv	AT elenatrizna improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT dianabaidamshina improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT annagorshkova improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT valentindrucker improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT mikhailbogachev improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT antontikhonov improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup AT airatkayumov improvingtheefficacyofantimicrobialsagainstbiofilmembeddedbacteriausingbovinehyaluronidaseazoximerlongidazasupsup
_version_	1718410868752908288

Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza<sup>®</sup>)

Ejemplares similares