FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains

Abstract Platelet-derived growth factor receptor (PDGFR) senses extracellular growth factors and transfer the signals inside the cells regulating cell proliferation, migration and survival. It has been controversial at which membrane microdomains PDGFRs reside and how they control such diverse intra...

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Autores principales:	Jihye Seong, Min Huang, Kyoung Mi Sim, Hyunbin Kim, Yingxiao Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7b1f343f0644c62b19da8aeeb3d4935
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spelling	oai:doaj.org-article:e7b1f343f0644c62b19da8aeeb3d49352021-12-02T12:32:14ZFRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains10.1038/s41598-017-01789-y2045-2322https://doaj.org/article/e7b1f343f0644c62b19da8aeeb3d49352017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01789-yhttps://doaj.org/toc/2045-2322Abstract Platelet-derived growth factor receptor (PDGFR) senses extracellular growth factors and transfer the signals inside the cells regulating cell proliferation, migration and survival. It has been controversial at which membrane microdomains PDGFRs reside and how they control such diverse intracellular signaling pathways. Here, we developed a novel PDGFR biosensor based on fluorescence resonance energy transfer (FRET), which can detect the real-time PDGFR activity in live cells with high spatiotemporal resolutions. To study subcellular PDGFR activity at membrane microdomains, this PDGFR biosensor was further targeted in or outside lipid rafts via different lipid modification signals. The results suggest that, in response to PDGF stimulation, PDGFR activity is evenly distributed at different membrane microdomains, while integrin-mediated signaling events have inhibitory effects on the activation of PDGFR specifically located in lipid rafts but not outside rafts, implying the role of lipid microdomains as segregated signaling platforms.Jihye SeongMin HuangKyoung Mi SimHyunbin KimYingxiao WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jihye Seong Min Huang Kyoung Mi Sim Hyunbin Kim Yingxiao Wang FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
description	Abstract Platelet-derived growth factor receptor (PDGFR) senses extracellular growth factors and transfer the signals inside the cells regulating cell proliferation, migration and survival. It has been controversial at which membrane microdomains PDGFRs reside and how they control such diverse intracellular signaling pathways. Here, we developed a novel PDGFR biosensor based on fluorescence resonance energy transfer (FRET), which can detect the real-time PDGFR activity in live cells with high spatiotemporal resolutions. To study subcellular PDGFR activity at membrane microdomains, this PDGFR biosensor was further targeted in or outside lipid rafts via different lipid modification signals. The results suggest that, in response to PDGF stimulation, PDGFR activity is evenly distributed at different membrane microdomains, while integrin-mediated signaling events have inhibitory effects on the activation of PDGFR specifically located in lipid rafts but not outside rafts, implying the role of lipid microdomains as segregated signaling platforms.
format	article
author	Jihye Seong Min Huang Kyoung Mi Sim Hyunbin Kim Yingxiao Wang
author_facet	Jihye Seong Min Huang Kyoung Mi Sim Hyunbin Kim Yingxiao Wang
author_sort	Jihye Seong
title	FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
title_short	FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
title_full	FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
title_fullStr	FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
title_full_unstemmed	FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
title_sort	fret-based visualization of pdgf receptor activation at membrane microdomains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e7b1f343f0644c62b19da8aeeb3d4935
work_keys_str_mv	AT jihyeseong fretbasedvisualizationofpdgfreceptoractivationatmembranemicrodomains AT minhuang fretbasedvisualizationofpdgfreceptoractivationatmembranemicrodomains AT kyoungmisim fretbasedvisualizationofpdgfreceptoractivationatmembranemicrodomains AT hyunbinkim fretbasedvisualizationofpdgfreceptoractivationatmembranemicrodomains AT yingxiaowang fretbasedvisualizationofpdgfreceptoractivationatmembranemicrodomains
_version_	1718394190532968448

FRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains

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