Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont

ABSTRACT Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs,...

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Autores principales:	Sasisekhar Bennuru, James A. Cotton, Jose M. C. Ribeiro, Alexandra Grote, Bhavana Harsha, Nancy Holroyd, Amruta Mhashilkar, Douglas M. Molina, Arlo Z. Randall, Adam D. Shandling, Thomas R. Unnasch, Elodie Ghedin, Matthew Berriman, Sara Lustigman, Thomas B. Nutman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e7b6042546d449e9810ec83ffa50a946
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spelling	oai:doaj.org-article:e7b6042546d449e9810ec83ffa50a9462021-11-15T15:50:16ZStage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont10.1128/mBio.02028-162150-7511https://doaj.org/article/e7b6042546d449e9810ec83ffa50a9462016-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02028-16https://doaj.org/toc/2150-7511ABSTRACT Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite’s adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. IMPORTANCE The global onchocerciasis (river blindness) elimination program will have to rely on the development of new tools (drugs, vaccines, biomarkers) to achieve its goals by 2025. As an adjunct to the completed genomic sequencing of O. volvulus, we used a comprehensive proteomic and transcriptomic profiling strategy to gain a comprehensive understanding of both the vector-derived and human host-derived parasite stages. In so doing, we have identified proteins and pathways that enable novel drug targeting studies and the discovery of novel vaccine candidates, as well as useful biomarkers of active infection.Sasisekhar BennuruJames A. CottonJose M. C. RibeiroAlexandra GroteBhavana HarshaNancy HolroydAmruta MhashilkarDouglas M. MolinaArlo Z. RandallAdam D. ShandlingThomas R. UnnaschElodie GhedinMatthew BerrimanSara LustigmanThomas B. NutmanAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 6 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sasisekhar Bennuru James A. Cotton Jose M. C. Ribeiro Alexandra Grote Bhavana Harsha Nancy Holroyd Amruta Mhashilkar Douglas M. Molina Arlo Z. Randall Adam D. Shandling Thomas R. Unnasch Elodie Ghedin Matthew Berriman Sara Lustigman Thomas B. Nutman Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
description	ABSTRACT Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite’s adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. IMPORTANCE The global onchocerciasis (river blindness) elimination program will have to rely on the development of new tools (drugs, vaccines, biomarkers) to achieve its goals by 2025. As an adjunct to the completed genomic sequencing of O. volvulus, we used a comprehensive proteomic and transcriptomic profiling strategy to gain a comprehensive understanding of both the vector-derived and human host-derived parasite stages. In so doing, we have identified proteins and pathways that enable novel drug targeting studies and the discovery of novel vaccine candidates, as well as useful biomarkers of active infection.
format	article
author	Sasisekhar Bennuru James A. Cotton Jose M. C. Ribeiro Alexandra Grote Bhavana Harsha Nancy Holroyd Amruta Mhashilkar Douglas M. Molina Arlo Z. Randall Adam D. Shandling Thomas R. Unnasch Elodie Ghedin Matthew Berriman Sara Lustigman Thomas B. Nutman
author_facet	Sasisekhar Bennuru James A. Cotton Jose M. C. Ribeiro Alexandra Grote Bhavana Harsha Nancy Holroyd Amruta Mhashilkar Douglas M. Molina Arlo Z. Randall Adam D. Shandling Thomas R. Unnasch Elodie Ghedin Matthew Berriman Sara Lustigman Thomas B. Nutman
author_sort	Sasisekhar Bennuru
title	Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
title_short	Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
title_full	Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
title_fullStr	Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
title_full_unstemmed	Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont
title_sort	stage-specific transcriptome and proteome analyses of the filarial parasite <named-content content-type="genus-species">onchocerca volvulus</named-content> and its <italic toggle="yes">wolbachia</italic> endosymbiont
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/e7b6042546d449e9810ec83ffa50a946
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Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite <named-content content-type="genus-species">Onchocerca volvulus</named-content> and Its <italic toggle="yes">Wolbachia</italic> Endosymbiont

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